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A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase

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A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase. / Perez-Mendoza, Daniel; Coulthurst, Sarah J.; Humphris, Sonia; Campbell, Emma; Welch, Martin; Toth, Ian K.; Salmond, George P. C.

In: Molecular Microbiology, Vol. 82, No. 3, 11.2011, p. 719-733.

Research output: Contribution to journalArticle

Harvard

Perez-Mendoza, D, Coulthurst, SJ, Humphris, S, Campbell, E, Welch, M, Toth, IK & Salmond, GPC 2011, 'A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase' Molecular Microbiology, vol 82, no. 3, pp. 719-733., 10.1111/j.1365-2958.2011.07849.x

APA

Perez-Mendoza, D., Coulthurst, S. J., Humphris, S., Campbell, E., Welch, M., Toth, I. K., & Salmond, G. P. C. (2011). A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase. Molecular Microbiology, 82(3), 719-733. 10.1111/j.1365-2958.2011.07849.x

Vancouver

Perez-Mendoza D, Coulthurst SJ, Humphris S, Campbell E, Welch M, Toth IK et al. A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase. Molecular Microbiology. 2011 Nov;82(3):719-733. Available from: 10.1111/j.1365-2958.2011.07849.x

Author

Perez-Mendoza, Daniel; Coulthurst, Sarah J.; Humphris, Sonia; Campbell, Emma; Welch, Martin; Toth, Ian K.; Salmond, George P. C. / A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase.

In: Molecular Microbiology, Vol. 82, No. 3, 11.2011, p. 719-733.

Research output: Contribution to journalArticle

Bibtex - Download

@article{58203233ca334d07ae97d4dc920a9635,
title = "A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase",
keywords = "CYCLIC-DI-GMP, PSEUDOMONAS-FLUORESCENS WCS365, CAROTOVORA SUBSP ATROSEPTICA, BIOFILM FORMATION, RESPONSE REGULATOR, ESCHERICHIA-COLI, CAULOBACTER-CRESCENTUS, EXTRACELLULAR-MATRIX, ALLOSTERIC CONTROL, PUTIDA-BIOFILMS",
author = "Daniel Perez-Mendoza and Coulthurst, {Sarah J.} and Sonia Humphris and Emma Campbell and Martin Welch and Toth, {Ian K.} and Salmond, {George P. C.}",
year = "2011",
doi = "10.1111/j.1365-2958.2011.07849.x",
volume = "82",
number = "3",
pages = "719--733",
journal = "Molecular Microbiology",
issn = "0950-382x",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A multi-repeat adhesin of the phytopathogen, Pectobacterium atrosepticum, is secreted by a Type I pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase

A1 - Perez-Mendoza,Daniel

A1 - Coulthurst,Sarah J.

A1 - Humphris,Sonia

A1 - Campbell,Emma

A1 - Welch,Martin

A1 - Toth,Ian K.

A1 - Salmond,George P. C.

AU - Perez-Mendoza,Daniel

AU - Coulthurst,Sarah J.

AU - Humphris,Sonia

AU - Campbell,Emma

AU - Welch,Martin

AU - Toth,Ian K.

AU - Salmond,George P. C.

PY - 2011/11

Y1 - 2011/11

N2 - <p>Cyclic diguanylate (c-di-GMP) is a second messenger controlling many important bacterial processes. The phytopathogen Pectobacterium atrosepticum SCRI1043 (Pba1043) possesses a Type I secretion system (T1SS) essential for the secretion of a proteinaceous multi-repeat adhesin (MRP) required for binding to the host plant. The genes encoding the MRP and the T1SS are tightly linked to genes encoding several putative c-di-GMP regulatory components. We show that c-di-GMP regulates secreted MRP levels in Pba1043 through the action of two genes encoding predicted diguanylate cyclase (DGC) and phosphodiesterase proteins (ECA3270 and ECA3271). Phenotypic analyses and quantification of c-di-GMP levels demonstrated that ECA3270 and ECA3271 regulate secreted MRP levels by increasing and decreasing, respectively, the intracellular levels of c-di-GMP. Moreover, ECA3270 represents the first active DGC reported to have an alternative active-site motif from the 'canonical' GG[D/E]EF. ECA3270 has an A-site motif of SGDEF and analysis of single amino acid replacements demonstrated that the first position of this motif can tolerate functional substitution. Serine in position one of the A-site is also observed in many other DGCs. Finally, another T1SS-linked regulator (ECA3265) also plays an important role in regulating secreted MRP, with an altered localization of MRP observed in an ECA3265 mutant background. Mutants defective in these three T1SS-linked regulators exhibit a reduction in root binding and virulence, confirming that this complex, finely tuned regulation system is crucial in the interaction with host plants.</p>

AB - <p>Cyclic diguanylate (c-di-GMP) is a second messenger controlling many important bacterial processes. The phytopathogen Pectobacterium atrosepticum SCRI1043 (Pba1043) possesses a Type I secretion system (T1SS) essential for the secretion of a proteinaceous multi-repeat adhesin (MRP) required for binding to the host plant. The genes encoding the MRP and the T1SS are tightly linked to genes encoding several putative c-di-GMP regulatory components. We show that c-di-GMP regulates secreted MRP levels in Pba1043 through the action of two genes encoding predicted diguanylate cyclase (DGC) and phosphodiesterase proteins (ECA3270 and ECA3271). Phenotypic analyses and quantification of c-di-GMP levels demonstrated that ECA3270 and ECA3271 regulate secreted MRP levels by increasing and decreasing, respectively, the intracellular levels of c-di-GMP. Moreover, ECA3270 represents the first active DGC reported to have an alternative active-site motif from the 'canonical' GG[D/E]EF. ECA3270 has an A-site motif of SGDEF and analysis of single amino acid replacements demonstrated that the first position of this motif can tolerate functional substitution. Serine in position one of the A-site is also observed in many other DGCs. Finally, another T1SS-linked regulator (ECA3265) also plays an important role in regulating secreted MRP, with an altered localization of MRP observed in an ECA3265 mutant background. Mutants defective in these three T1SS-linked regulators exhibit a reduction in root binding and virulence, confirming that this complex, finely tuned regulation system is crucial in the interaction with host plants.</p>

KW - CYCLIC-DI-GMP

KW - PSEUDOMONAS-FLUORESCENS WCS365

KW - CAROTOVORA SUBSP ATROSEPTICA

KW - BIOFILM FORMATION

KW - RESPONSE REGULATOR

KW - ESCHERICHIA-COLI

KW - CAULOBACTER-CRESCENTUS

KW - EXTRACELLULAR-MATRIX

KW - ALLOSTERIC CONTROL

KW - PUTIDA-BIOFILMS

U2 - 10.1111/j.1365-2958.2011.07849.x

DO - 10.1111/j.1365-2958.2011.07849.x

M1 - Article

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382x

IS - 3

VL - 82

SP - 719

EP - 733

ER -

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