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Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics

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Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. / Abdullah, A.; Kitteringham, N.R.; Jenkins, R.E.; Goldring, C.; Higgins, L.; Yamamoto, M.; Hayes, J.; Park, B.K.

In: Pharmacological Reports, Vol. 64, No. 3, 2012, p. 680-697.

Research output: Contribution to journalArticle

Harvard

Abdullah, A, Kitteringham, NR, Jenkins, RE, Goldring, C, Higgins, L, Yamamoto, M, Hayes, J & Park, BK 2012, 'Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics' Pharmacological Reports, vol 64, no. 3, pp. 680-697.

APA

Abdullah, A., Kitteringham, N. R., Jenkins, R. E., Goldring, C., Higgins, L., Yamamoto, M., Hayes, J., & Park, B. K. (2012). Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. Pharmacological Reports, 64(3), 680-697

Vancouver

Abdullah A, Kitteringham NR, Jenkins RE, Goldring C, Higgins L, Yamamoto M et al. Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. Pharmacological Reports. 2012;64(3):680-697.

Author

Abdullah, A.; Kitteringham, N.R.; Jenkins, R.E.; Goldring, C.; Higgins, L.; Yamamoto, M.; Hayes, J.; Park, B.K. / Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics.

In: Pharmacological Reports, Vol. 64, No. 3, 2012, p. 680-697.

Research output: Contribution to journalArticle

Bibtex - Download

@article{4302f9dc7a1f4104be1d8e241870c8f9,
title = "Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics",
author = "A. Abdullah and N.R. Kitteringham and R.E. Jenkins and C. Goldring and L. Higgins and M. Yamamoto and J. Hayes and B.K. Park",
year = "2012",
volume = "64",
number = "3",
pages = "680--697",
journal = "Pharmacological Reports",
issn = "1734-1140",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics

A1 - Abdullah,A.

A1 - Kitteringham,N.R.

A1 - Jenkins,R.E.

A1 - Goldring,C.

A1 - Higgins,L.

A1 - Yamamoto,M.

A1 - Hayes,J.

A1 - Park,B.K.

AU - Abdullah,A.

AU - Kitteringham,N.R.

AU - Jenkins,R.E.

AU - Goldring,C.

AU - Higgins,L.

AU - Yamamoto,M.

AU - Hayes,J.

AU - Park,B.K.

PY - 2012

Y1 - 2012

N2 - Background: The transcription factor Nrf2 regulates expression of multiple cellular defence proteins through the antioxidant response element (ARE). Nrf2-deficient mice (Nrf2 ) are highly susceptible to xenobiotic-mediated toxicity, but it is not known whether this reflects low basal expression or reduced inducibility of Nrf2-regulated genes in response to chemical insults. Methods: Wild type and Nrf2 mice were fed diet supplemented with the established Nrf2 inducer butylated hydroxyanisole (BHA) [0.5% (w/w)] for 14 days. To define the range of Nrf2-regulated proteins, both basally and following exposure to BHA, a comparison of the liver proteomes of Nrf2 and wild type mice was conducted. The two-dimensional gel electrophoresis (2-DE) technique and MALDI mass spectrometry were utilized in the attempt to define Nrf2-regulated proteins. Results: Overall, 24 proteins were identified, which were regulated either basally (3 proteins), inducibly (16 proteins), or both (5 proteins). These included several well-established Nrf2-driven gene products e.g., aldo-keto reductase and glutathione transferases. Multiple consensus ARE/ARE-like sequences were found in the Nrf2-regulated genes. Conclusions: This study confirms the central role of Nrf2 in the induction of multiple defense proteins as well as its control in the constitutive expression of certain proteins. Copyright © 2012 by Institute of Pharmacology Polish Academy of Sciences.

AB - Background: The transcription factor Nrf2 regulates expression of multiple cellular defence proteins through the antioxidant response element (ARE). Nrf2-deficient mice (Nrf2 ) are highly susceptible to xenobiotic-mediated toxicity, but it is not known whether this reflects low basal expression or reduced inducibility of Nrf2-regulated genes in response to chemical insults. Methods: Wild type and Nrf2 mice were fed diet supplemented with the established Nrf2 inducer butylated hydroxyanisole (BHA) [0.5% (w/w)] for 14 days. To define the range of Nrf2-regulated proteins, both basally and following exposure to BHA, a comparison of the liver proteomes of Nrf2 and wild type mice was conducted. The two-dimensional gel electrophoresis (2-DE) technique and MALDI mass spectrometry were utilized in the attempt to define Nrf2-regulated proteins. Results: Overall, 24 proteins were identified, which were regulated either basally (3 proteins), inducibly (16 proteins), or both (5 proteins). These included several well-established Nrf2-driven gene products e.g., aldo-keto reductase and glutathione transferases. Multiple consensus ARE/ARE-like sequences were found in the Nrf2-regulated genes. Conclusions: This study confirms the central role of Nrf2 in the induction of multiple defense proteins as well as its control in the constitutive expression of certain proteins. Copyright © 2012 by Institute of Pharmacology Polish Academy of Sciences.

UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84866136122&md5=8a020f1d730d9c15575853e34a120cb2

M1 - Article

JO - Pharmacological Reports

JF - Pharmacological Reports

SN - 1734-1140

IS - 3

VL - 64

SP - 680

EP - 697

ER -

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