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Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics

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Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. / Abdullah, A.; Kitteringham, N.R.; Jenkins, R.E.; Goldring, C.; Higgins, L.; Yamamoto, M.; Hayes, J.; Park, B.K.

In: Pharmacological Reports, Vol. 64, No. 3, 2012, p. 680-697.

Research output: Contribution to journalArticle

Harvard

Abdullah, A, Kitteringham, NR, Jenkins, RE, Goldring, C, Higgins, L, Yamamoto, M, Hayes, J & Park, BK 2012, 'Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics' Pharmacological Reports, vol 64, no. 3, pp. 680-697.

APA

Abdullah, A., Kitteringham, N. R., Jenkins, R. E., Goldring, C., Higgins, L., Yamamoto, M., ... Park, B. K. (2012). Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. Pharmacological Reports, 64(3), 680-697.

Vancouver

Abdullah A, Kitteringham NR, Jenkins RE, Goldring C, Higgins L, Yamamoto M et al. Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics. Pharmacological Reports. 2012;64(3):680-697.

Author

Abdullah, A.; Kitteringham, N.R.; Jenkins, R.E.; Goldring, C.; Higgins, L.; Yamamoto, M.; Hayes, J.; Park, B.K. / Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics.

In: Pharmacological Reports, Vol. 64, No. 3, 2012, p. 680-697.

Research output: Contribution to journalArticle

Bibtex - Download

@article{4302f9dc7a1f4104be1d8e241870c8f9,
title = "Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics",
author = "A. Abdullah and N.R. Kitteringham and R.E. Jenkins and C. Goldring and L. Higgins and M. Yamamoto and J. Hayes and B.K. Park",
year = "2012",
volume = "64",
number = "3",
pages = "680--697",
journal = "Pharmacological Reports",
issn = "1734-1140",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics

A1 - Abdullah,A.

A1 - Kitteringham,N.R.

A1 - Jenkins,R.E.

A1 - Goldring,C.

A1 - Higgins,L.

A1 - Yamamoto,M.

A1 - Hayes,J.

A1 - Park,B.K.

AU - Abdullah,A.

AU - Kitteringham,N.R.

AU - Jenkins,R.E.

AU - Goldring,C.

AU - Higgins,L.

AU - Yamamoto,M.

AU - Hayes,J.

AU - Park,B.K.

PY - 2012

Y1 - 2012

N2 - Background: The transcription factor Nrf2 regulates expression of multiple cellular defence proteins through the antioxidant response element (ARE). Nrf2-deficient mice (Nrf2 ) are highly susceptible to xenobiotic-mediated toxicity, but it is not known whether this reflects low basal expression or reduced inducibility of Nrf2-regulated genes in response to chemical insults. Methods: Wild type and Nrf2 mice were fed diet supplemented with the established Nrf2 inducer butylated hydroxyanisole (BHA) [0.5% (w/w)] for 14 days. To define the range of Nrf2-regulated proteins, both basally and following exposure to BHA, a comparison of the liver proteomes of Nrf2 and wild type mice was conducted. The two-dimensional gel electrophoresis (2-DE) technique and MALDI mass spectrometry were utilized in the attempt to define Nrf2-regulated proteins. Results: Overall, 24 proteins were identified, which were regulated either basally (3 proteins), inducibly (16 proteins), or both (5 proteins). These included several well-established Nrf2-driven gene products e.g., aldo-keto reductase and glutathione transferases. Multiple consensus ARE/ARE-like sequences were found in the Nrf2-regulated genes. Conclusions: This study confirms the central role of Nrf2 in the induction of multiple defense proteins as well as its control in the constitutive expression of certain proteins. Copyright © 2012 by Institute of Pharmacology Polish Academy of Sciences.

AB - Background: The transcription factor Nrf2 regulates expression of multiple cellular defence proteins through the antioxidant response element (ARE). Nrf2-deficient mice (Nrf2 ) are highly susceptible to xenobiotic-mediated toxicity, but it is not known whether this reflects low basal expression or reduced inducibility of Nrf2-regulated genes in response to chemical insults. Methods: Wild type and Nrf2 mice were fed diet supplemented with the established Nrf2 inducer butylated hydroxyanisole (BHA) [0.5% (w/w)] for 14 days. To define the range of Nrf2-regulated proteins, both basally and following exposure to BHA, a comparison of the liver proteomes of Nrf2 and wild type mice was conducted. The two-dimensional gel electrophoresis (2-DE) technique and MALDI mass spectrometry were utilized in the attempt to define Nrf2-regulated proteins. Results: Overall, 24 proteins were identified, which were regulated either basally (3 proteins), inducibly (16 proteins), or both (5 proteins). These included several well-established Nrf2-driven gene products e.g., aldo-keto reductase and glutathione transferases. Multiple consensus ARE/ARE-like sequences were found in the Nrf2-regulated genes. Conclusions: This study confirms the central role of Nrf2 in the induction of multiple defense proteins as well as its control in the constitutive expression of certain proteins. Copyright © 2012 by Institute of Pharmacology Polish Academy of Sciences.

UR - http://www.scopus.com/inward/record.url?scp=84866136122&partnerID=8YFLogxK

M1 - Article

JO - Pharmacological Reports

JF - Pharmacological Reports

SN - 1734-1140

IS - 3

VL - 64

SP - 680

EP - 697

ER -

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