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Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169

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Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169. / Chen, W.C.; Kawasaki, N.; Nycholat, C.M.; Han, S.; Pilotte, J.; Paulson, J.C.; Crocker, P.R.

In: PLoS ONE, Vol. 7, No. 6, e39039, 19.06.2012.

Research output: Contribution to journalArticle

Harvard

Chen, WC, Kawasaki, N, Nycholat, CM, Han, S, Pilotte, J, Paulson, JC & Crocker, PR 2012, 'Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169' PLoS ONE, vol 7, no. 6, e39039., 10.1371/journal.pone.0039039

APA

Chen, W. C., Kawasaki, N., Nycholat, C. M., Han, S., Pilotte, J., Paulson, J. C., & Crocker, P. R. (2012). Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169. PLoS ONE, 7(6), [e39039]. 10.1371/journal.pone.0039039

Vancouver

Chen WC, Kawasaki N, Nycholat CM, Han S, Pilotte J, Paulson JC et al. Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169. PLoS ONE. 2012 Jun 19;7(6). e39039. Available from: 10.1371/journal.pone.0039039

Author

Chen, W.C.; Kawasaki, N.; Nycholat, C.M.; Han, S.; Pilotte, J.; Paulson, J.C.; Crocker, P.R. / Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169.

In: PLoS ONE, Vol. 7, No. 6, e39039, 19.06.2012.

Research output: Contribution to journalArticle

Bibtex - Download

@article{bbf884da55d64ff486595dd1f1867d97,
title = "Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169",
author = "W.C. Chen and N. Kawasaki and C.M. Nycholat and S. Han and J. Pilotte and J.C. Paulson and P.R. Crocker",
year = "2012",
doi = "10.1371/journal.pone.0039039",
volume = "7",
number = "6",
journal = "PLoS ONE",
issn = "1932-6203",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Antigen delivery to macrophages using liposomal nanoparticles targeting Sialoadhesin/CD169

A1 - Chen,W.C.

A1 - Kawasaki,N.

A1 - Nycholat,C.M.

A1 - Han,S.

A1 - Pilotte,J.

A1 - Paulson,J.C.

A1 - Crocker,P.R.

AU - Chen,W.C.

AU - Kawasaki,N.

AU - Nycholat,C.M.

AU - Han,S.

AU - Pilotte,J.

AU - Paulson,J.C.

AU - Crocker,P.R.

PY - 2012/6/19

Y1 - 2012/6/19

N2 - Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinity glycan ligands of Sn. The Sn-targeted liposomes selectively bind to and are internalized by Sn-expressing cells, and accumulate intracellularly over time. Our results show that ligand decorated liposomes are specific for Sn, since they are taken up by bone marrow derived macrophages that are derived from wild type but not Sn mice. Importantly, the Sn-targeted liposomes dramatically enhance the delivery of antigens to macrophages for presentation to and proliferation of antigen-specific T cells. Together, these data provide insights into the potential of cell-specific targeting and delivery of antigens to intracellular organelles of macrophages using Sn-ligand decorated liposomal nanoparticles. © 2012 Chen et al.

AB - Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinity glycan ligands of Sn. The Sn-targeted liposomes selectively bind to and are internalized by Sn-expressing cells, and accumulate intracellularly over time. Our results show that ligand decorated liposomes are specific for Sn, since they are taken up by bone marrow derived macrophages that are derived from wild type but not Sn mice. Importantly, the Sn-targeted liposomes dramatically enhance the delivery of antigens to macrophages for presentation to and proliferation of antigen-specific T cells. Together, these data provide insights into the potential of cell-specific targeting and delivery of antigens to intracellular organelles of macrophages using Sn-ligand decorated liposomal nanoparticles. © 2012 Chen et al.

UR - http://www.scopus.com/inward/record.url?scp=84862585040&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0039039

DO - 10.1371/journal.pone.0039039

M1 - Article

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

VL - 7

ER -

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