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Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation

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Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation. / Chen, Changwei; Boylan, Michael; Evans, Caroline A.; Whettan, Anthony D.; Wright, Eric.

In: Journal of Proteome Research, Vol. 4, No. 4, 2005, p. 1371-1380.

Research output: Contribution to journalArticle

Harvard

Chen, C, Boylan, M, Evans, CA, Whettan, AD & Wright, E 2005, 'Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation' Journal of Proteome Research, vol 4, no. 4, pp. 1371-1380.

APA

Chen, C., Boylan, M., Evans, C. A., Whettan, A. D., & Wright, E. (2005). Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation. Journal of Proteome Research, 4(4), 1371-1380doi: 10.1021/pr050067r

Vancouver

Chen C, Boylan M, Evans CA, Whettan AD, Wright E. Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation. Journal of Proteome Research. 2005;4(4):1371-1380.

Author

Chen, Changwei; Boylan, Michael; Evans, Caroline A.; Whettan, Anthony D.; Wright, Eric / Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation.

In: Journal of Proteome Research, Vol. 4, No. 4, 2005, p. 1371-1380.

Research output: Contribution to journalArticle

Bibtex - Download

@article{d70f0a2169bc4fdf9a1c15071eef80f7,
title = "Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation",
author = "Changwei Chen and Michael Boylan and Evans, {Caroline A.} and Whettan, {Anthony D.} and Eric Wright",
note = "dc.publisher: American Chemical Society",
year = "2005",
volume = "4",
number = "4",
pages = "1371--1380",
journal = "Journal of Proteome Research",
issn = "1535-3893",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation

A1 - Chen,Changwei

A1 - Boylan,Michael

A1 - Evans,Caroline A.

A1 - Whettan,Anthony D.

A1 - Wright,Eric

AU - Chen,Changwei

AU - Boylan,Michael

AU - Evans,Caroline A.

AU - Whettan,Anthony D.

AU - Wright,Eric

PY - 2005

Y1 - 2005

N2 - NOTE: THE MATHEMATICAL SYMBOLS/SPECIAL CHARACTERS/IMAGES IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT IN THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY.A flow cytometric protocol was developed to isolate primary bone marrow resident macrophages (CD11b(-) Gr-1(-) F4/80(+)) before and 24 h after 0.5 Gy ?-irradiation from mouse strains (C57BL/6 and CBA/Ca) that exhibit significant differences in the response of their hematopoietic tissues to ionizing radiation. The proteins from these populations were analyzed using two-dimensional difference gel electrophoresis (2D DIGE) and mass spectrometry. We identified 36 macrophage proteins from 52 spots in both C57BL/6 and CBA/Ca. Thirty-three spots showed significant difference between genotypes and 16 of them corresponding to 11 proteins were identified. These included G-protein signaling 16, glucose-regulated protein 78, and lactoylglutathione lyase. We detected 16 and 18 spot changes following irradiation in C57BL/6 and CBA/Ca respectively, and in total 16 of them were identified. The identified proteins included calreticulin, lactoylglutathione lyase, regulator of G-protein signaling 16 and peroxiredoxin 5, mitochondrial precursor. The application of DIGE to primary bone marrow resident macrophages has allowed the first description of the proteome of these important components of the hematopoietic microenvironment and an analysis of their in vivo response to ionizing radiation which may shed light on the mechanism underlying the differential radiation-induced leukemogenesis exhibited within these mouse strains.

AB - NOTE: THE MATHEMATICAL SYMBOLS/SPECIAL CHARACTERS/IMAGES IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT IN THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY.A flow cytometric protocol was developed to isolate primary bone marrow resident macrophages (CD11b(-) Gr-1(-) F4/80(+)) before and 24 h after 0.5 Gy ?-irradiation from mouse strains (C57BL/6 and CBA/Ca) that exhibit significant differences in the response of their hematopoietic tissues to ionizing radiation. The proteins from these populations were analyzed using two-dimensional difference gel electrophoresis (2D DIGE) and mass spectrometry. We identified 36 macrophage proteins from 52 spots in both C57BL/6 and CBA/Ca. Thirty-three spots showed significant difference between genotypes and 16 of them corresponding to 11 proteins were identified. These included G-protein signaling 16, glucose-regulated protein 78, and lactoylglutathione lyase. We detected 16 and 18 spot changes following irradiation in C57BL/6 and CBA/Ca respectively, and in total 16 of them were identified. The identified proteins included calreticulin, lactoylglutathione lyase, regulator of G-protein signaling 16 and peroxiredoxin 5, mitochondrial precursor. The application of DIGE to primary bone marrow resident macrophages has allowed the first description of the proteome of these important components of the hematopoietic microenvironment and an analysis of their in vivo response to ionizing radiation which may shed light on the mechanism underlying the differential radiation-induced leukemogenesis exhibited within these mouse strains.

KW - Bone marrow

KW - Flow cytometry

KW - Ionizing radiation

KW - Macrophage proteome

KW - Two-dimensional difference gel electrophoresis

U2 - 10.1021/pr050067r

DO - 10.1021/pr050067r

M1 - Article

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 4

VL - 4

SP - 1371

EP - 1380

ER -

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