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Cannabinoid receptors in submandibular acinar cells

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Cannabinoid receptors in submandibular acinar cells : functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling. / Kopach, Olga; Vats, Juliana; Netsyk, Olga; Voitenko, Nana; Irving, Andrew; Fedirko, Nataliya.

In: Journal of Cell Science, Vol. 125, No. 8, 15.04.2012, p. 1884-1895.

Research output: Contribution to journalArticle

Harvard

Kopach, O, Vats, J, Netsyk, O, Voitenko, N, Irving, A & Fedirko, N 2012, 'Cannabinoid receptors in submandibular acinar cells: functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling' Journal of Cell Science, vol 125, no. 8, pp. 1884-1895., 10.1242/jcs.088930

APA

Kopach, O., Vats, J., Netsyk, O., Voitenko, N., Irving, A., & Fedirko, N. (2012). Cannabinoid receptors in submandibular acinar cells: functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling. Journal of Cell Science, 125(8), 1884-1895. 10.1242/jcs.088930

Vancouver

Kopach O, Vats J, Netsyk O, Voitenko N, Irving A, Fedirko N. Cannabinoid receptors in submandibular acinar cells: functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling. Journal of Cell Science. 2012 Apr 15;125(8):1884-1895. Available from: 10.1242/jcs.088930

Author

Kopach, Olga; Vats, Juliana; Netsyk, Olga; Voitenko, Nana; Irving, Andrew; Fedirko, Nataliya / Cannabinoid receptors in submandibular acinar cells : functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling.

In: Journal of Cell Science, Vol. 125, No. 8, 15.04.2012, p. 1884-1895.

Research output: Contribution to journalArticle

Bibtex - Download

@article{bd2e909383ea45879f03f4f0ad558f55,
title = "Cannabinoid receptors in submandibular acinar cells: functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling",
author = "Olga Kopach and Juliana Vats and Olga Netsyk and Nana Voitenko and Andrew Irving and Nataliya Fedirko",
year = "2012",
doi = "10.1242/jcs.088930",
volume = "125",
number = "8",
pages = "1884--1895",
journal = "Journal of Cell Science",
issn = "0021-9533",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cannabinoid receptors in submandibular acinar cells

T2 - functional coupling between saliva fluid and electrolytes secretion and Ca2+ signalling

A1 - Kopach,Olga

A1 - Vats,Juliana

A1 - Netsyk,Olga

A1 - Voitenko,Nana

A1 - Irving,Andrew

A1 - Fedirko,Nataliya

AU - Kopach,Olga

AU - Vats,Juliana

AU - Netsyk,Olga

AU - Voitenko,Nana

AU - Irving,Andrew

AU - Fedirko,Nataliya

PY - 2012/4/15

Y1 - 2012/4/15

N2 - Cannabinoid receptors (CBRs) belong to the G protein-coupled receptor superfamily, and activation of CBRs in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes in acinar cell physiology and in intracellular signalling remains unclear. Here, we uncover functional CB(1)Rs and CB(2)Rs in acinar cells of rat submandibular gland and their essential role in saliva secretion. Pharmacological activation of CB(1)Rs and CB(2)Rs in the submandibular gland suppressed saliva outflow and modified saliva content produced by the submandibular gland in vivo. Using Na+-selective microelectrodes to record secretory Na+ responses in the lumen of acini, we observed a reduction in Na+ transport following the activation of CBRs, which was counteracted by the selective CB1R antagonist AM251. In addition, activation of CB(1)Rs or CB(2)Rs caused inhibition of Na+-K+-ATPase activity in microsomes derived from the gland tissue as well as in isolated acinar cells. Using a Ca2+ imaging technique, we showed that activation of CB(1)Rs and CB(2)Rs alters [Ca2+](cyt) signalling in acinar cells by distinct pathways, involving Ca2+ release from the endoplasmic reticulum (ER) and store-operated Ca2+ entry (SOCE), respectively. Our data demonstrate the expression of CB(1)Rs and CB(2)Rs in acinar cells, and their involvement in the regulation of salivary gland functioning.</p>

AB - Cannabinoid receptors (CBRs) belong to the G protein-coupled receptor superfamily, and activation of CBRs in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes in acinar cell physiology and in intracellular signalling remains unclear. Here, we uncover functional CB(1)Rs and CB(2)Rs in acinar cells of rat submandibular gland and their essential role in saliva secretion. Pharmacological activation of CB(1)Rs and CB(2)Rs in the submandibular gland suppressed saliva outflow and modified saliva content produced by the submandibular gland in vivo. Using Na+-selective microelectrodes to record secretory Na+ responses in the lumen of acini, we observed a reduction in Na+ transport following the activation of CBRs, which was counteracted by the selective CB1R antagonist AM251. In addition, activation of CB(1)Rs or CB(2)Rs caused inhibition of Na+-K+-ATPase activity in microsomes derived from the gland tissue as well as in isolated acinar cells. Using a Ca2+ imaging technique, we showed that activation of CB(1)Rs and CB(2)Rs alters [Ca2+](cyt) signalling in acinar cells by distinct pathways, involving Ca2+ release from the endoplasmic reticulum (ER) and store-operated Ca2+ entry (SOCE), respectively. Our data demonstrate the expression of CB(1)Rs and CB(2)Rs in acinar cells, and their involvement in the regulation of salivary gland functioning.</p>

U2 - 10.1242/jcs.088930

DO - 10.1242/jcs.088930

M1 - Article

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 8

VL - 125

SP - 1884

EP - 1895

ER -

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