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Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state

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Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state. / Calabrese, Vittorio; Signorile, Anna; Cornelius, Carolin; Mancuso, Cesare; Scapagnini, Giovanni; Ventimiglia, Bernardo; Ragusa, Nicolo; Dinkova-Kostova, Albena.

Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling. ed. / Enrique Cadenas; Lester Packer. San Diego : Academic Press, 2008. p. 83-110 (Methods in Enzymology; Vol. 441).

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Harvard

Calabrese, V, Signorile, A, Cornelius, C, Mancuso, C, Scapagnini, G, Ventimiglia, B, Ragusa, N & Dinkova-Kostova, A 2008, 'Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state'. in E Cadenas & L Packer (eds), Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling. Methods in Enzymology, vol. 441, Academic Press, San Diego, pp. 83-110., 10.1016/S0076-6879(08)01206-8

APA

Calabrese, V., Signorile, A., Cornelius, C., Mancuso, C., Scapagnini, G., Ventimiglia, B., ... Dinkova-Kostova, A. (2008). Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state. In E. Cadenas, & L. Packer (Eds.), Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling. (pp. 83-110). (Methods in Enzymology; Vol. 441). San Diego: Academic Press. 10.1016/S0076-6879(08)01206-8

Vancouver

Calabrese V, Signorile A, Cornelius C, Mancuso C, Scapagnini G, Ventimiglia B et al. Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state. In Cadenas E, Packer L, editors, Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling. San Diego: Academic Press. 2008. p. 83-110. (Methods in Enzymology). Available from: 10.1016/S0076-6879(08)01206-8

Author

Calabrese, Vittorio; Signorile, Anna; Cornelius, Carolin; Mancuso, Cesare; Scapagnini, Giovanni; Ventimiglia, Bernardo; Ragusa, Nicolo; Dinkova-Kostova, Albena / Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state.

Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling. ed. / Enrique Cadenas; Lester Packer. San Diego : Academic Press, 2008. p. 83-110 (Methods in Enzymology; Vol. 441).

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Bibtex - Download

@inbook{27373bb8de2b42a2a81eb2285ffaba87,
title = "Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state",
keywords = "NITRIC-OXIDE SYNTHASE, CELLULAR STRESS-RESPONSE, KAPPA-B ACTIVATION, HEME OXYGENASE-1 INDUCTION, COLORECTAL-CARCINOMA CELLS, VASCULAR ENDOTHELIAL-CELLS, HSP70 GENE-EXPRESSION, RAT GLIAL-CELLS, OXIDATIVE-STRESS, NEURODEGENERATIVE DISORDERS",
publisher = "Academic Press",
author = "Vittorio Calabrese and Anna Signorile and Carolin Cornelius and Cesare Mancuso and Giovanni Scapagnini and Bernardo Ventimiglia and Nicolo Ragusa and Albena Dinkova-Kostova",
year = "2008",
doi = "10.1016/S0076-6879(08)01206-8",
editor = "Enrique Cadenas and Lester Packer",
isbn = "9780123743091",
series = "Methods in Enzymology",
pages = "83-110",
booktitle = "Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling",

}

RIS (suitable for import to EndNote) - Download

TY - CHAP

T1 - Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state

A1 - Calabrese,Vittorio

A1 - Signorile,Anna

A1 - Cornelius,Carolin

A1 - Mancuso,Cesare

A1 - Scapagnini,Giovanni

A1 - Ventimiglia,Bernardo

A1 - Ragusa,Nicolo

A1 - Dinkova-Kostova,Albena

AU - Calabrese,Vittorio

AU - Signorile,Anna

AU - Cornelius,Carolin

AU - Mancuso,Cesare

AU - Scapagnini,Giovanni

AU - Ventimiglia,Bernardo

AU - Ragusa,Nicolo

AU - Dinkova-Kostova,Albena

PB - Academic Press

CY - San Diego

PY - 2008

Y1 - 2008

N2 - <p>The products of vitagenes such as heat shock protein 32 (Hsp32, heme oxygenase 1) and Hsp70, the family of inducible cytoprotective proteins regulated by the Keap1/Nrf2/ARE pathway, and small molecule antioxidants such as glutathione provide the cell with powerful means to counteract and survive various conditions of stress. Among these protective systems, the heat shock proteins represent a highly conserved and robust way for preservation of correct protein conformation, recovery of damaged proteins, and cell survival. Their regulation is dependent on the redox status of the cell, thus redox regulation is rapidly evolving as an important metabolic modulator of cellular functions, and is being increasingly implicated in many chronic inflammatory and degenerative diseases. Protein thiols play a key role in redox sensing, and regulation of cellular redox state is crucial mediator of multiple metabolic, signalling and transcriptional processes in the brain. Nitric oxide, and reactive nitrogen species induce the transcription of vitagenes and Keap1/Nrf2/ARE-dependent genes whose functional products protect against a wide array of subsequent challenges. Emerging interest is now focusing on exogenous small molecules that are capable of activating these systems as a novel target to minimize deleterious consequences associated with free radical-induced cell damage, such as during neurodegeneration. This chapter describes methods that can be used to assess the expression of heat shock proteins and the cellular glutathione redox status and discusses their relevance to mechanisms modulating the onset and progression of neurodegenerative diseases.</p>

AB - <p>The products of vitagenes such as heat shock protein 32 (Hsp32, heme oxygenase 1) and Hsp70, the family of inducible cytoprotective proteins regulated by the Keap1/Nrf2/ARE pathway, and small molecule antioxidants such as glutathione provide the cell with powerful means to counteract and survive various conditions of stress. Among these protective systems, the heat shock proteins represent a highly conserved and robust way for preservation of correct protein conformation, recovery of damaged proteins, and cell survival. Their regulation is dependent on the redox status of the cell, thus redox regulation is rapidly evolving as an important metabolic modulator of cellular functions, and is being increasingly implicated in many chronic inflammatory and degenerative diseases. Protein thiols play a key role in redox sensing, and regulation of cellular redox state is crucial mediator of multiple metabolic, signalling and transcriptional processes in the brain. Nitric oxide, and reactive nitrogen species induce the transcription of vitagenes and Keap1/Nrf2/ARE-dependent genes whose functional products protect against a wide array of subsequent challenges. Emerging interest is now focusing on exogenous small molecules that are capable of activating these systems as a novel target to minimize deleterious consequences associated with free radical-induced cell damage, such as during neurodegeneration. This chapter describes methods that can be used to assess the expression of heat shock proteins and the cellular glutathione redox status and discusses their relevance to mechanisms modulating the onset and progression of neurodegenerative diseases.</p>

KW - NITRIC-OXIDE SYNTHASE

KW - CELLULAR STRESS-RESPONSE

KW - KAPPA-B ACTIVATION

KW - HEME OXYGENASE-1 INDUCTION

KW - COLORECTAL-CARCINOMA CELLS

KW - VASCULAR ENDOTHELIAL-CELLS

KW - HSP70 GENE-EXPRESSION

KW - RAT GLIAL-CELLS

KW - OXIDATIVE-STRESS

KW - NEURODEGENERATIVE DISORDERS

UR - http://www.scopus.com/inward/record.url?scp=52249099052&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(08)01206-8

DO - 10.1016/S0076-6879(08)01206-8

M3 - Other chapter contribution

SN - 9780123743091

BT - Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling

T2 - Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling

A2 - Packer,Lester

ED - Packer,Lester

T3 - Methods in Enzymology

T3 - en_GB

SP - 83

EP - 110

ER -

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