Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment. / Fu, Wenxiang; Tao, Wei; Zheng, Puwei; Fu, Jingyan; Bian, Minglei; Jiang, Qing; Clarke, Paul R.; Zhang, Chuanmao.
In: Journal of Cell Science, Vol. 123, No. 21, 01.11.2010, p. 3645-3651.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment
A1 - Fu,Wenxiang
A1 - Tao,Wei
A1 - Zheng,Puwei
A1 - Fu,Jingyan
A1 - Bian,Minglei
A1 - Jiang,Qing
A1 - Clarke,Paul R.
A1 - Zhang,Chuanmao
AU - Fu,Wenxiang
AU - Tao,Wei
AU - Zheng,Puwei
AU - Fu,Jingyan
AU - Bian,Minglei
AU - Jiang,Qing
AU - Clarke,Paul R.
AU - Zhang,Chuanmao
PY - 2010/11/1
Y1 - 2010/11/1
N2 - <p>Transforming acidic coiled-coil-containing protein 3 (TACC3) has been implicated in mitotic spindle assembly, although the mechanisms involved are largely unknown. Here we identify that clathrin heavy chain (CHC) binds specifically to phosphorylated TACC3 and recruits it to spindle poles for proper spindle assembly and chromosome alignment. Phosphorylation of Xenopus TACC3 at serine 620 (S620) and S626, but not S33, is required for its binding with CHC. Knockdown of CHC by RNA interference (RNAi) abolishes the targeting of TACC3 to spindle poles and results in abnormal spindle assembly and chromosome misalignment, similar to the defects caused by TACC3 knockdown. Furthermore, the binding of CHC with phosphorylated TACC3 is inhibited by importin. and this inhibition is reversed by the presence of the GTP-binding nuclear protein Ran in the GTP-bound state. Together, these results indicate that the recruitment of phosphorylated TACC3 to spindle poles by CHC ensures proper spindle assembly and chromosome alignment, and is regulated by Ran.</p>
AB - <p>Transforming acidic coiled-coil-containing protein 3 (TACC3) has been implicated in mitotic spindle assembly, although the mechanisms involved are largely unknown. Here we identify that clathrin heavy chain (CHC) binds specifically to phosphorylated TACC3 and recruits it to spindle poles for proper spindle assembly and chromosome alignment. Phosphorylation of Xenopus TACC3 at serine 620 (S620) and S626, but not S33, is required for its binding with CHC. Knockdown of CHC by RNA interference (RNAi) abolishes the targeting of TACC3 to spindle poles and results in abnormal spindle assembly and chromosome misalignment, similar to the defects caused by TACC3 knockdown. Furthermore, the binding of CHC with phosphorylated TACC3 is inhibited by importin. and this inhibition is reversed by the presence of the GTP-binding nuclear protein Ran in the GTP-bound state. Together, these results indicate that the recruitment of phosphorylated TACC3 to spindle poles by CHC ensures proper spindle assembly and chromosome alignment, and is regulated by Ran.</p>
KW - TACC3
KW - Clathrin
KW - Phosphorylation
KW - Spindle assembly
KW - Chromosome alignment
KW - LAEVIS EGG EXTRACTS
KW - MITOTIC SPINDLE
KW - MEDIATED ENDOCYTOSIS
KW - AURORA-A
KW - PROTEIN
KW - MASKIN
KW - RCC1
KW - RAN
KW - MITOSIS
KW - FAMILY
U2 - 10.1242/jcs.075911
DO - 10.1242/jcs.075911
M1 - Article
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 21
VL - 123
SP - 3645
EP - 3651
ER -