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Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment

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Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment. / Fu, Wenxiang; Tao, Wei; Zheng, Puwei; Fu, Jingyan; Bian, Minglei; Jiang, Qing; Clarke, Paul R.; Zhang, Chuanmao.

In: Journal of Cell Science, Vol. 123, No. 21, 01.11.2010, p. 3645-3651.

Research output: Contribution to journalArticle

Harvard

Fu, W, Tao, W, Zheng, P, Fu, J, Bian, M, Jiang, Q, Clarke, PR & Zhang, C 2010, 'Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment' Journal of Cell Science, vol 123, no. 21, pp. 3645-3651., 10.1242/jcs.075911

APA

Fu, W., Tao, W., Zheng, P., Fu, J., Bian, M., Jiang, Q., ... Zhang, C. (2010). Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment. Journal of Cell Science, 123(21), 3645-3651. 10.1242/jcs.075911

Vancouver

Fu W, Tao W, Zheng P, Fu J, Bian M, Jiang Q et al. Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment. Journal of Cell Science. 2010 Nov 1;123(21):3645-3651. Available from: 10.1242/jcs.075911

Author

Fu, Wenxiang; Tao, Wei; Zheng, Puwei; Fu, Jingyan; Bian, Minglei; Jiang, Qing; Clarke, Paul R.; Zhang, Chuanmao / Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment.

In: Journal of Cell Science, Vol. 123, No. 21, 01.11.2010, p. 3645-3651.

Research output: Contribution to journalArticle

Bibtex - Download

@article{04c5be454ca44c719b5088a36a74a479,
title = "Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment",
keywords = "TACC3, Clathrin, Phosphorylation, Spindle assembly, Chromosome alignment, LAEVIS EGG EXTRACTS, MITOTIC SPINDLE, MEDIATED ENDOCYTOSIS, AURORA-A, PROTEIN, MASKIN, RCC1, RAN, MITOSIS, FAMILY",
author = "Wenxiang Fu and Wei Tao and Puwei Zheng and Jingyan Fu and Minglei Bian and Qing Jiang and Clarke, {Paul R.} and Chuanmao Zhang",
year = "2010",
doi = "10.1242/jcs.075911",
volume = "123",
number = "21",
pages = "3645--3651",
journal = "Journal of Cell Science",
issn = "0021-9533",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Clathrin recruits phosphorylated TACC3 to spindle poles for bipolar spindle assembly and chromosome alignment

A1 - Fu,Wenxiang

A1 - Tao,Wei

A1 - Zheng,Puwei

A1 - Fu,Jingyan

A1 - Bian,Minglei

A1 - Jiang,Qing

A1 - Clarke,Paul R.

A1 - Zhang,Chuanmao

AU - Fu,Wenxiang

AU - Tao,Wei

AU - Zheng,Puwei

AU - Fu,Jingyan

AU - Bian,Minglei

AU - Jiang,Qing

AU - Clarke,Paul R.

AU - Zhang,Chuanmao

PY - 2010/11/1

Y1 - 2010/11/1

N2 - <p>Transforming acidic coiled-coil-containing protein 3 (TACC3) has been implicated in mitotic spindle assembly, although the mechanisms involved are largely unknown. Here we identify that clathrin heavy chain (CHC) binds specifically to phosphorylated TACC3 and recruits it to spindle poles for proper spindle assembly and chromosome alignment. Phosphorylation of Xenopus TACC3 at serine 620 (S620) and S626, but not S33, is required for its binding with CHC. Knockdown of CHC by RNA interference (RNAi) abolishes the targeting of TACC3 to spindle poles and results in abnormal spindle assembly and chromosome misalignment, similar to the defects caused by TACC3 knockdown. Furthermore, the binding of CHC with phosphorylated TACC3 is inhibited by importin. and this inhibition is reversed by the presence of the GTP-binding nuclear protein Ran in the GTP-bound state. Together, these results indicate that the recruitment of phosphorylated TACC3 to spindle poles by CHC ensures proper spindle assembly and chromosome alignment, and is regulated by Ran.</p>

AB - <p>Transforming acidic coiled-coil-containing protein 3 (TACC3) has been implicated in mitotic spindle assembly, although the mechanisms involved are largely unknown. Here we identify that clathrin heavy chain (CHC) binds specifically to phosphorylated TACC3 and recruits it to spindle poles for proper spindle assembly and chromosome alignment. Phosphorylation of Xenopus TACC3 at serine 620 (S620) and S626, but not S33, is required for its binding with CHC. Knockdown of CHC by RNA interference (RNAi) abolishes the targeting of TACC3 to spindle poles and results in abnormal spindle assembly and chromosome misalignment, similar to the defects caused by TACC3 knockdown. Furthermore, the binding of CHC with phosphorylated TACC3 is inhibited by importin. and this inhibition is reversed by the presence of the GTP-binding nuclear protein Ran in the GTP-bound state. Together, these results indicate that the recruitment of phosphorylated TACC3 to spindle poles by CHC ensures proper spindle assembly and chromosome alignment, and is regulated by Ran.</p>

KW - TACC3

KW - Clathrin

KW - Phosphorylation

KW - Spindle assembly

KW - Chromosome alignment

KW - LAEVIS EGG EXTRACTS

KW - MITOTIC SPINDLE

KW - MEDIATED ENDOCYTOSIS

KW - AURORA-A

KW - PROTEIN

KW - MASKIN

KW - RCC1

KW - RAN

KW - MITOSIS

KW - FAMILY

U2 - 10.1242/jcs.075911

DO - 10.1242/jcs.075911

M1 - Article

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 21

VL - 123

SP - 3645

EP - 3651

ER -

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