Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
| Original language | English |
|---|---|
| Title | Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B |
| Editors | Daniel Herschlag |
| Place of publication | San Diego |
| Publisher | Academic Press |
| Publication date | 2009 |
| Pages | 143-157 |
| Number of pages | 15 |
| Volume | 469 |
| ISBN (Print) | 9780123809223 |
| DOIs | |
| State | Published |
Comparative gel electrophoresis provides information on the relative angles subtended between helical arms at a branchpoint in RNA. It is based upon the comparison of electrophoretic mobility in polyacrylamide gels of species containing two long arms, with the remaining one(s) being significantly shorter. Although the method currently lacks a really well-established basis of physical theory, it is very powerful, yet simple to apply. It has had a number of significant successes in RNA, DNA and DNA-protein complexes, and in all cases to date the results have stood the test of time and eventual comparison with crystallographic analysis.