Discovery - University of Dundee - Online Publications

Library & Learning Centre

Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA

Standard

Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA. / Lilley, David M. J.

Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. ed. / Daniel Herschlag. Vol. 469 San Diego : Academic Press, 2009. p. 143-157.

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Harvard

Lilley, DMJ 2009, 'Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA'. in D Herschlag (ed.), Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. vol. 469, Academic Press, San Diego, pp. 143-157., 10.1016/S0076-6879(09)69007-8

APA

Lilley, D. M. J. (2009). Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA. In D. Herschlag (Ed.), Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. (Vol. 469, pp. 143-157). San Diego: Academic Press. 10.1016/S0076-6879(09)69007-8

Vancouver

Lilley DMJ. Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA. In Herschlag D, editor, Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. Vol. 469. San Diego: Academic Press. 2009. p. 143-157. Available from: 10.1016/S0076-6879(09)69007-8

Author

Lilley, David M. J. / Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA.

Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. ed. / Daniel Herschlag. Vol. 469 San Diego : Academic Press, 2009. p. 143-157.

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Bibtex - Download

@inbook{8cee23751c3d4cfba3d9538a7dceca8f,
title = "Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA",
keywords = "KINK-TURN RNA, 4-WAY DNA JUNCTION, HOLLIDAY JUNCTION, HAIRPIN RIBOZYME, CRYSTAL-STRUCTURE, GLOBAL STRUCTURE, REPTATION MODEL, T7 ENDONUCLEASE, NUCLEIC-ACIDS, BRANCHED DNA",
publisher = "Academic Press",
author = "Lilley, {David M. J.}",
year = "2009",
doi = "10.1016/S0076-6879(09)69007-8",
editor = "Daniel Herschlag",
volume = "469",
isbn = "9780123809223",
pages = "143-157",
booktitle = "Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B",

}

RIS (suitable for import to EndNote) - Download

TY - CHAP

T1 - Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA

A1 - Lilley,David M. J.

AU - Lilley,David M. J.

PB - Academic Press

CY - San Diego

PY - 2009

Y1 - 2009

N2 - <p>Comparative gel electrophoresis provides information on the relative angles subtended between helical arms at a branchpoint in RNA. It is based upon the comparison of electrophoretic mobility in polyacrylamide gels of species containing two long arms, with the remaining one(s) being significantly shorter. Although the method currently lacks a really well-established basis of physical theory, it is very powerful, yet simple to apply. It has had a number of significant successes in RNA, DNA and DNA-protein complexes, and in all cases to date the results have stood the test of time and eventual comparison with crystallographic analysis.</p>

AB - <p>Comparative gel electrophoresis provides information on the relative angles subtended between helical arms at a branchpoint in RNA. It is based upon the comparison of electrophoretic mobility in polyacrylamide gels of species containing two long arms, with the remaining one(s) being significantly shorter. Although the method currently lacks a really well-established basis of physical theory, it is very powerful, yet simple to apply. It has had a number of significant successes in RNA, DNA and DNA-protein complexes, and in all cases to date the results have stood the test of time and eventual comparison with crystallographic analysis.</p>

KW - KINK-TURN RNA

KW - 4-WAY DNA JUNCTION

KW - HOLLIDAY JUNCTION

KW - HAIRPIN RIBOZYME

KW - CRYSTAL-STRUCTURE

KW - GLOBAL STRUCTURE

KW - REPTATION MODEL

KW - T7 ENDONUCLEASE

KW - NUCLEIC-ACIDS

KW - BRANCHED DNA

U2 - 10.1016/S0076-6879(09)69007-8

DO - 10.1016/S0076-6879(09)69007-8

M1 - Other chapter contribution

SN - 9780123809223

VL - 469

BT - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B

T2 - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B

A2 - Herschlag,Daniel

ED - Herschlag,Daniel

SP - 143

EP - 157

ER -

Library & Learning Centre

Contact | Accessibility | Policy