Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA. / Lilley, David M. J.
Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. ed. / Daniel Herschlag. Vol. 469 San Diego : Academic Press, 2009. p. 143-157.Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
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TY - CHAP
T1 - Comparative Gel Electrophoresis Analysis of Helical Junctions in RNA
A1 - Lilley,David M. J.
AU - Lilley,David M. J.
PB - Academic Press
CY - San Diego
PY - 2009
Y1 - 2009
N2 - <p>Comparative gel electrophoresis provides information on the relative angles subtended between helical arms at a branchpoint in RNA. It is based upon the comparison of electrophoretic mobility in polyacrylamide gels of species containing two long arms, with the remaining one(s) being significantly shorter. Although the method currently lacks a really well-established basis of physical theory, it is very powerful, yet simple to apply. It has had a number of significant successes in RNA, DNA and DNA-protein complexes, and in all cases to date the results have stood the test of time and eventual comparison with crystallographic analysis.</p>
AB - <p>Comparative gel electrophoresis provides information on the relative angles subtended between helical arms at a branchpoint in RNA. It is based upon the comparison of electrophoretic mobility in polyacrylamide gels of species containing two long arms, with the remaining one(s) being significantly shorter. Although the method currently lacks a really well-established basis of physical theory, it is very powerful, yet simple to apply. It has had a number of significant successes in RNA, DNA and DNA-protein complexes, and in all cases to date the results have stood the test of time and eventual comparison with crystallographic analysis.</p>
KW - KINK-TURN RNA
KW - 4-WAY DNA JUNCTION
KW - HOLLIDAY JUNCTION
KW - HAIRPIN RIBOZYME
KW - CRYSTAL-STRUCTURE
KW - GLOBAL STRUCTURE
KW - REPTATION MODEL
KW - T7 ENDONUCLEASE
KW - NUCLEIC-ACIDS
KW - BRANCHED DNA
U2 - 10.1016/S0076-6879(09)69007-8
DO - 10.1016/S0076-6879(09)69007-8
M1 - Other chapter contribution
SN - 9780123809223
VL - 469
BT - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
T2 - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
A2 - Herschlag,Daniel
ED - Herschlag,Daniel
SP - 143
EP - 157
ER -