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Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays

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Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays. / Martin, Kirsty J.; Shpiro, Natalia; Traynor, Ryan; Elliott, Matthew; Arthur, J. Simon C.

In: Neuropharmacology, Vol. 61, No. 1-2, 2011, p. 148-155.

Research output: Contribution to journalArticle

Harvard

Martin, KJ, Shpiro, N, Traynor, R, Elliott, M & Arthur, JSC 2011, 'Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays' Neuropharmacology, vol 61, no. 1-2, pp. 148-155., 10.1016/j.neuropharm.2011.03.021

APA

Martin, K. J., Shpiro, N., Traynor, R., Elliott, M., & Arthur, J. S. C. (2011). Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays. Neuropharmacology, 61(1-2), 148-155. 10.1016/j.neuropharm.2011.03.021

Vancouver

Martin KJ, Shpiro N, Traynor R, Elliott M, Arthur JSC. Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays. Neuropharmacology. 2011;61(1-2):148-155. Available from: 10.1016/j.neuropharm.2011.03.021

Author

Martin, Kirsty J.; Shpiro, Natalia; Traynor, Ryan; Elliott, Matthew; Arthur, J. Simon C. / Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays.

In: Neuropharmacology, Vol. 61, No. 1-2, 2011, p. 148-155.

Research output: Contribution to journalArticle

Bibtex - Download

@article{c234301430654393ad359380d9e6280d,
title = "Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays",
author = "Martin, {Kirsty J.} and Natalia Shpiro and Ryan Traynor and Matthew Elliott and Arthur, {J. Simon C.}",
year = "2011",
doi = "10.1016/j.neuropharm.2011.03.021",
volume = "61",
number = "1-2",
pages = "148--155",
journal = "Neuropharmacology",
issn = "0028-3908",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Comparison of the specificity of Trk inhibitors in recombinant and neuronal assays

A1 - Martin,Kirsty J.

A1 - Shpiro,Natalia

A1 - Traynor,Ryan

A1 - Elliott,Matthew

A1 - Arthur,J. Simon C.

AU - Martin,Kirsty J.

AU - Shpiro,Natalia

AU - Traynor,Ryan

AU - Elliott,Matthew

AU - Arthur,J. Simon C.

PY - 2011

Y1 - 2011

N2 - <p>Neurotrophins are important mediators of neuronal development, survival and plasticity. They act via binding to Trk receptors, which results in the stimulation of the intracellular tyrosine kinase domain of the receptor leading to autophosphorylation of this domain. This in turn creates a scaffold that recruits various adapter proteins allowing the activation of intracellular signaling cascades including the PLC gamma, MAPK and PI3K pathways. Compounds that specifically block the activity of the tyrosine kinase domain of Irk receptors would provide a powerful tool to study the role of these receptors in cells. K252a has previously been used for this purpose, however we show here that it can inhibit many tyrosine and serine/threonine kinases in vitro. Profiling of 3 newer inhibitors, referred to here as SHN-753, SHN-722 and GSK-Trk, demonstrate that they have significantly improved specificity for the kinase activity of TrkA in vitro compared to K252a. In addition these compounds were found to block the TrkB mediated activation of ERK1/2 by BDNF, but did not affect NMDA induced ERK1/2 activation. These compounds, while still not completely specific for Trk receptor kinase activity, do represent a considerable improvement over K252a and should prove valuable in the study of neurotrophin-mediated actions in the nervous system. (C) 2011 Elsevier Ltd. All rights reserved.</p>

AB - <p>Neurotrophins are important mediators of neuronal development, survival and plasticity. They act via binding to Trk receptors, which results in the stimulation of the intracellular tyrosine kinase domain of the receptor leading to autophosphorylation of this domain. This in turn creates a scaffold that recruits various adapter proteins allowing the activation of intracellular signaling cascades including the PLC gamma, MAPK and PI3K pathways. Compounds that specifically block the activity of the tyrosine kinase domain of Irk receptors would provide a powerful tool to study the role of these receptors in cells. K252a has previously been used for this purpose, however we show here that it can inhibit many tyrosine and serine/threonine kinases in vitro. Profiling of 3 newer inhibitors, referred to here as SHN-753, SHN-722 and GSK-Trk, demonstrate that they have significantly improved specificity for the kinase activity of TrkA in vitro compared to K252a. In addition these compounds were found to block the TrkB mediated activation of ERK1/2 by BDNF, but did not affect NMDA induced ERK1/2 activation. These compounds, while still not completely specific for Trk receptor kinase activity, do represent a considerable improvement over K252a and should prove valuable in the study of neurotrophin-mediated actions in the nervous system. (C) 2011 Elsevier Ltd. All rights reserved.</p>

U2 - 10.1016/j.neuropharm.2011.03.021

DO - 10.1016/j.neuropharm.2011.03.021

M1 - Article

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 1-2

VL - 61

SP - 148

EP - 155

ER -

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