Delta 160p53 is a novel N-terminal p53 isoform encoded by Delta 133p53 transcript. / Marcel, Virginie; Perrier, Stephane; Aoubala, Mustapha; Ageorges, Sylvain; Groves, Michael J.; Diot, Alexandra; Fernandes, Kenneth; Tauro, Sudhir; Bourdon, Jean-Christophe.
In: FEBS Letters, Vol. 584, No. 21, 05.11.2010, p. 4463-4468.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Delta 160p53 is a novel N-terminal p53 isoform encoded by Delta 133p53 transcript
A1 - Marcel,Virginie
A1 - Perrier,Stephane
A1 - Aoubala,Mustapha
A1 - Ageorges,Sylvain
A1 - Groves,Michael J.
A1 - Diot,Alexandra
A1 - Fernandes,Kenneth
A1 - Tauro,Sudhir
A1 - Bourdon,Jean-Christophe
AU - Marcel,Virginie
AU - Perrier,Stephane
AU - Aoubala,Mustapha
AU - Ageorges,Sylvain
AU - Groves,Michael J.
AU - Diot,Alexandra
AU - Fernandes,Kenneth
AU - Tauro,Sudhir
AU - Bourdon,Jean-Christophe
PY - 2010/11/5
Y1 - 2010/11/5
N2 - <p>p53 gene expresses several protein isoforms modulating p53-mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, Delta 160p53, lacking the first 159 residues. By knockdown experiments and site-directed mutagenesis, we show that Delta 160p53 is encoded by Delta 133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of Delta 160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and Delta 133p53 protein expression but not the one of Delta 160p53. Overall, these results show that the Delta 133p53 transcript generates two different p53 isoforms, Delta 133p53 and Delta 160p53. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</p>
AB - <p>p53 gene expresses several protein isoforms modulating p53-mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, Delta 160p53, lacking the first 159 residues. By knockdown experiments and site-directed mutagenesis, we show that Delta 160p53 is encoded by Delta 133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of Delta 160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and Delta 133p53 protein expression but not the one of Delta 160p53. Overall, these results show that the Delta 133p53 transcript generates two different p53 isoforms, Delta 133p53 and Delta 160p53. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</p>
KW - p53
KW - Tumour suppressor
KW - Delta 160p53
KW - Delta 133p53
KW - CELLS
KW - GENE
U2 - 10.1016/j.febslet.2010.10.005
DO - 10.1016/j.febslet.2010.10.005
M1 - Article
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 21
VL - 584
SP - 4463
EP - 4468
ER -