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Detecting and Segmenting Nanodiscs in Immuno-Electron Micrographs

Detecting and Segmenting Nanodiscs in Immuno-Electron Micrographs

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Original languageEnglish
Title of host publicationMedical Image Understanding and Analysis
Subtitle of host publicationMIUA
EditorsConstantino Reyes-Aldasoro, Greg Slabaugh
Place of PublicationUnited Kingdom
PublisherBritish Machine Vision Association and Society for Pattern Recognition
Pages53-58
Number of pages6
ISBN (Print)1901725510
StatePublished - Jul 2014
Event18th Annual Conference in Medical Image Understanding and Analysis - London, United Kingdom

Conference

Conference18th Annual Conference in Medical Image Understanding and Analysis
Abbreviated titleMIUA 2014
CountryUnited Kingdom
CityLondon
Period9/07/1411/07/14
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Abstract

Nanodiscs are soluble nanoscale phospholipid bilayers with applications in drug delivery and the study of membrane proteins, for example. They can be imaged using electron microscopy, along with immunogold markers indicating locations of proteins of interest. We describe and evaluate methods for automatically detecting and segmenting nanodiscs in electron micrographs. The detection method modifies aspects of the Fast Radial Symmetry Transform to detect nanodiscs that exhibit approximate radial symmetry against noisy but predominantly lighter background. Detected nanodiscs are then segmented using radial active contours. Experiments on micrographs both with and without immunogold markers indicate promising detection and segmentation performance, and that information on nanodisc quantities, locations, size distributions, and co-location with proteins of interest could be extracted automatically.

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    ©2014. The copyright of this document resides with its authors.
    It may be distributed unchanged freely in print or electronic forms.

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