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Differential contextual responses of normal human breast epithelium to Ionizing radiation in a mouse xenograft model

Differential contextual responses of normal human breast epithelium to Ionizing radiation in a mouse xenograft model

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Authors

  • Philip J. Coates (Lead / Corresponding author)
  • M. Virginia C. L. Appleyard
  • Karen Murray
  • Caroline Ackland
  • June Gardner
  • Douglas C. Brown
  • Dougal J. A. Adamson
  • Lee B. Jordan
  • Colin A. Purdie
  • Alastair J. Munro
  • Eric G. Wright
  • John A. Dewar
  • Alastair M. Thompson

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Info

Original languageEnglish
Pages9808-9815
Number of pages8
JournalCancer Research
Journal publication date1 Dec 2010
Journal number23
Volume70
DOIs
StatePublished

Abstract

Radiotherapy is a key treatment option for breast cancer, yet the molecular responses of normal human breast epithelial cells to ionizing radiation are unclear. A murine subcutaneous xenograft model was developed in which nonneoplastic human breast tissue was maintained with the preservation of normal tissue architecture, allowing us to study for the first time the radiation response of normal human breast tissue in situ. Ionizing radiation induced dose-dependent p53 stabilization and p53 phosphorylation, together with the induction of p21(CDKN1A) and apoptosis of normal breast epithelium. Although p53 was stabilized in both luminal and basal cells, induction of Ser392-phosphorylated p53 and p21 was higher in basal cells and varied along the length of the ductal system. Basal breast epithelial cells expressed Delta Np63, which was unchanged on irradiation. Although stromal responses themselves were minimal, the response of normal breast epithelium to ionizing radiation differed according to the stromal setting. We also demonstrated a dose-dependent induction of gamma-H2AX foci in epithelial cells that was similarly dependent on the stromal environment and differed between basal and luminal epithelial cells. The intrinsic differences between human mammary cell types in response to in vivo irradiation are consistent with clinical observation that therapeutic ionizing radiation is associated with the development of basal-type breast carcinomas. Furthermore, there may be clinically important stromal-epithelial interactions that influence DNA damage responses in the normal breast. These findings demonstrate highly complex responses of normal human breast epithelium following ionizing radiation exposure and emphasize the importance of studying whole-tissue effects rather than single-cell systems. Cancer Res; 70( 23); 9808-15. (C)2010 AACR.

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