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Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors

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Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors. / Baines, A. E.; Correa, S. A. L.; Irving, A.J.; Frenguelli, B. G.

In: Neuropharmacology, Vol. 61, No. 1-2, 2011, p. 1-11.

Research output: Contribution to journalArticle

Harvard

Baines, AE, Correa, SAL, Irving, AJ & Frenguelli, BG 2011, 'Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors' Neuropharmacology, vol 61, no. 1-2, pp. 1-11., 10.1016/j.neuropharm.2011.02.005

APA

Baines, A. E., Correa, S. A. L., Irving, A. J., & Frenguelli, B. G. (2011). Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors. Neuropharmacology, 61(1-2), 1-11. 10.1016/j.neuropharm.2011.02.005

Vancouver

Baines AE, Correa SAL, Irving AJ, Frenguelli BG. Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors. Neuropharmacology. 2011;61(1-2):1-11. Available from: 10.1016/j.neuropharm.2011.02.005

Author

Baines, A. E.; Correa, S. A. L.; Irving, A.J.; Frenguelli, B. G. / Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors.

In: Neuropharmacology, Vol. 61, No. 1-2, 2011, p. 1-11.

Research output: Contribution to journalArticle

Bibtex - Download

@article{9bfbffeab435485d91eba9a668f08806,
title = "Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors",
keywords = "Adenosine receptor, Internalisation, Trafficking, Epilepsy, Seizure, Ischaemia, AGONIST-DEPENDENT PHOSPHORYLATION, TEMPORAL-LOBE EPILEPSY, POSTISCHEMIC RAT-BRAIN, A(2A) RECEPTOR, A(3) RECEPTORS, SYNAPTIC-TRANSMISSION, NERVOUS-SYSTEM, A(1) RECEPTORS, MULTIPLE MECHANISMS, CALCIUM-CHANNELS",
author = "Baines, {A. E.} and Correa, {S. A. L.} and A.J. Irving and Frenguelli, {B. G.}",
year = "2011",
doi = "10.1016/j.neuropharm.2011.02.005",
volume = "61",
number = "1-2",
pages = "1--11",
journal = "Neuropharmacology",
issn = "0028-3908",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Differential trafficking of adenosine receptors in hippocampal neurons monitored using GFP- and super-ecliptic pHluorin-tagged receptors

A1 - Baines,A. E.

A1 - Correa,S. A. L.

A1 - Irving,A.J.

A1 - Frenguelli,B. G.

AU - Baines,A. E.

AU - Correa,S. A. L.

AU - Irving,A.J.

AU - Frenguelli,B. G.

PY - 2011

Y1 - 2011

N2 - <p>Adenosine receptors (ARs) modulate many cellular and systems-level processes in the mammalian CNS. However, little is known about the trafficking of ARs in neurons, despite their importance in controlling seizure activity and in neuroprotection in cerebral ischaemia. To address this we examined the agonist-dependent internalisation of C-terminal GFP-tagged A(1)Rs, A(2A)Rs and A(3)Rs in primary hippocampal neurons. Furthermore, we developed a novel super-ecliptic pHluorin (SEP)-tagged AIR which, via the N-terminal SEP tag, reports the cell-surface expression and trafficking of A(1)Rs in real-time. We demonstrate the differential trafficking of ARs in neurons: A(3)Rs internalise more rapidly than A(1)Rs, with little evidence of appreciable A(2A)R trafficking over the time-course of the experiments. Furthermore, the novel SEP-A(1)R construct revealed the time-course of internalisation and recovery of cell-surface expression to occur within minutes of agonist exposure and removal, respectively. These observations highlight the labile nature of A(1)R and A(3)Rs when expressed at the neuronal plasma membrane. Given the high levels of adenosine in the brain during ischaemia and seizures, internalisation of the inhibitory A(1)R may result in hyperexcitability, increased brain damage and the development of chronic epileptic states. (C) 2011 Elsevier Ltd. All rights reserved.</p>

AB - <p>Adenosine receptors (ARs) modulate many cellular and systems-level processes in the mammalian CNS. However, little is known about the trafficking of ARs in neurons, despite their importance in controlling seizure activity and in neuroprotection in cerebral ischaemia. To address this we examined the agonist-dependent internalisation of C-terminal GFP-tagged A(1)Rs, A(2A)Rs and A(3)Rs in primary hippocampal neurons. Furthermore, we developed a novel super-ecliptic pHluorin (SEP)-tagged AIR which, via the N-terminal SEP tag, reports the cell-surface expression and trafficking of A(1)Rs in real-time. We demonstrate the differential trafficking of ARs in neurons: A(3)Rs internalise more rapidly than A(1)Rs, with little evidence of appreciable A(2A)R trafficking over the time-course of the experiments. Furthermore, the novel SEP-A(1)R construct revealed the time-course of internalisation and recovery of cell-surface expression to occur within minutes of agonist exposure and removal, respectively. These observations highlight the labile nature of A(1)R and A(3)Rs when expressed at the neuronal plasma membrane. Given the high levels of adenosine in the brain during ischaemia and seizures, internalisation of the inhibitory A(1)R may result in hyperexcitability, increased brain damage and the development of chronic epileptic states. (C) 2011 Elsevier Ltd. All rights reserved.</p>

KW - Adenosine receptor

KW - Internalisation

KW - Trafficking

KW - Epilepsy

KW - Seizure

KW - Ischaemia

KW - AGONIST-DEPENDENT PHOSPHORYLATION

KW - TEMPORAL-LOBE EPILEPSY

KW - POSTISCHEMIC RAT-BRAIN

KW - A(2A) RECEPTOR

KW - A(3) RECEPTORS

KW - SYNAPTIC-TRANSMISSION

KW - NERVOUS-SYSTEM

KW - A(1) RECEPTORS

KW - MULTIPLE MECHANISMS

KW - CALCIUM-CHANNELS

U2 - 10.1016/j.neuropharm.2011.02.005

DO - 10.1016/j.neuropharm.2011.02.005

M1 - Article

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 1-2

VL - 61

SP - 1

EP - 11

ER -

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