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Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections

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Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections. / Tan, Khay M. (Lead / Corresponding author); Herrington, C. Simon; Brown, Christian T. A.

In: Journal of Biophotonics, Vol. 4, No. 1-2, 01.2011, p. 40-48.

Research output: Contribution to journalArticle

Harvard

Tan, KM, Herrington, CS & Brown, CTA 2011, 'Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections' Journal of Biophotonics, vol 4, no. 1-2, pp. 40-48., 10.1002/jbio.201000083

APA

Tan, K. M., Herrington, C. S., & Brown, C. T. A. (2011). Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections. Journal of Biophotonics, 4(1-2), 40-48. 10.1002/jbio.201000083

Vancouver

Tan KM, Herrington CS, Brown CTA. Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections. Journal of Biophotonics. 2011 Jan;4(1-2):40-48. Available from: 10.1002/jbio.201000083

Author

Tan, Khay M. (Lead / Corresponding author); Herrington, C. Simon; Brown, Christian T. A. / Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections.

In: Journal of Biophotonics, Vol. 4, No. 1-2, 01.2011, p. 40-48.

Research output: Contribution to journalArticle

Bibtex - Download

@article{94e83200450446408aa13609b02988c2,
title = "Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections",
keywords = "Raman spectroscopy, hierarchical cluster analysis, multivariate analysis, histopathology, VIBRATIONAL SPECTROSCOPY, LUNG-TISSUE, FTIR, MICROSPECTROSCOPY, METHODOLOGY, PATHOLOGY",
author = "Tan, {Khay M.} and Herrington, {C. Simon} and Brown, {Christian T. A.}",
year = "2011",
doi = "10.1002/jbio.201000083",
volume = "4",
number = "1-2",
pages = "40--48",
journal = "Journal of Biophotonics",
issn = "1864-063X",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histrological tissue sections

A1 - Tan,Khay M.

A1 - Herrington,C. Simon

A1 - Brown,Christian T. A.

AU - Tan,Khay M.

AU - Herrington,C. Simon

AU - Brown,Christian T. A.

PY - 2011/1

Y1 - 2011/1

N2 - <p>The authors present Raman cluster mapping of de-paraffinized normal cervical tissue and demonstrate the ability of this approach to differentiate between normal squamous epithelium and cervical intraepithelial neoplasia (CIN). Multivariate analysis was performed by hierarchical cluster analysis (HCA) of the Raman spectra associated with the different tissue types and Raman maps were generated using the resultant clusters. Using normal cervical tissue, squamous epithelium and the epithelial-stromal interface, a muscular artery and endocervical glands were successfully mapped. Analysis of a tissue section containing a cervical intraepithelial neoplasia (CIN) grade 2 lesion adjacent to normal squamous epithelium demonstrated that the ON lesion clustered predominantly with the basal epithelial cells of normal epithelium and allowed visual discrimination of these areas using the Raman map. These findings suggest that Raman mapping has the potential to provide images that are useful for disease diagnosis. In particular, the discrimination between normal cervical squamous epithelium and CIN is of relevance to cervical screening pathology.</p>

AB - <p>The authors present Raman cluster mapping of de-paraffinized normal cervical tissue and demonstrate the ability of this approach to differentiate between normal squamous epithelium and cervical intraepithelial neoplasia (CIN). Multivariate analysis was performed by hierarchical cluster analysis (HCA) of the Raman spectra associated with the different tissue types and Raman maps were generated using the resultant clusters. Using normal cervical tissue, squamous epithelium and the epithelial-stromal interface, a muscular artery and endocervical glands were successfully mapped. Analysis of a tissue section containing a cervical intraepithelial neoplasia (CIN) grade 2 lesion adjacent to normal squamous epithelium demonstrated that the ON lesion clustered predominantly with the basal epithelial cells of normal epithelium and allowed visual discrimination of these areas using the Raman map. These findings suggest that Raman mapping has the potential to provide images that are useful for disease diagnosis. In particular, the discrimination between normal cervical squamous epithelium and CIN is of relevance to cervical screening pathology.</p>

KW - Raman spectroscopy

KW - hierarchical cluster analysis

KW - multivariate analysis

KW - histopathology

KW - VIBRATIONAL SPECTROSCOPY

KW - LUNG-TISSUE

KW - FTIR

KW - MICROSPECTROSCOPY

KW - METHODOLOGY

KW - PATHOLOGY

UR - http://www.scopus.com/inward/record.url?scp=78650250506&partnerID=8YFLogxK

U2 - 10.1002/jbio.201000083

DO - 10.1002/jbio.201000083

M1 - Article

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 1-2

VL - 4

SP - 40

EP - 48

ER -

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