Diversity oriented syntheses of some furo[2,3-d]pyrimidines and pyrrolo[2,3-d] pyrimidines related to folate, guanine, and diaminopyrimidine-containing drugs have been developed for the preparation of potential anti-infective and anticancer compounds. Amide couplings and Suzuki couplings on the basic heterocyclic templates were used, in the latter case yields being especially high using aromatic trifluoroborates as the coupling partner. A new ring synthesis of 6-aryl-substituted deazaguanines bearing 2-alkylthio groups has been developed using Michael addition of substituted nitrostyrenes. Diversity at C-2 has been introduced by oxidation and substitution with a range of amino nucleophiles. The chemical reactivity of these pyrrolopyrimidines with respect to both electrophilic substitution in ring synthesis and nucleophilic substitution for diversity is discussed. Several compounds were found to inhibit pteridine reductases from the protozoan parasites Trypanosoma brucei and Leishmania major at the micromolar level and to inhibit the growth of Trypanosma brucei brucei in cell culture at higher concentrations. From these results, significant structural features required for inhibition of this important drug target enzyme have been identified.
AB -Diversity oriented syntheses of some furo[2,3-d]pyrimidines and pyrrolo[2,3-d] pyrimidines related to folate, guanine, and diaminopyrimidine-containing drugs have been developed for the preparation of potential anti-infective and anticancer compounds. Amide couplings and Suzuki couplings on the basic heterocyclic templates were used, in the latter case yields being especially high using aromatic trifluoroborates as the coupling partner. A new ring synthesis of 6-aryl-substituted deazaguanines bearing 2-alkylthio groups has been developed using Michael addition of substituted nitrostyrenes. Diversity at C-2 has been introduced by oxidation and substitution with a range of amino nucleophiles. The chemical reactivity of these pyrrolopyrimidines with respect to both electrophilic substitution in ring synthesis and nucleophilic substitution for diversity is discussed. Several compounds were found to inhibit pteridine reductases from the protozoan parasites Trypanosoma brucei and Leishmania major at the micromolar level and to inhibit the growth of Trypanosma brucei brucei in cell culture at higher concentrations. From these results, significant structural features required for inhibition of this important drug target enzyme have been identified.
KW - DIHYDROFOLATE-REDUCTASE INHIBITORS KW - CROSS-COUPLING REACTIONS KW - TYROSINE KINASE INHIBITORS KW - SOLID-PHASE SYNTHESIS KW - BIOLOGICAL EVALUATION KW - THYMIDYLATE SYNTHASE KW - RECEPTOR ANTAGONISTS KW - SELECTIVE INHIBITORS KW - CONVENIENT SYNTHESIS KW - ANTITUMOR AGENTS U2 - 10.1039/b818339b DO - 10.1039/b818339b M1 - Article JO - Organic and Biomolecular Chemistry JF - Organic and Biomolecular Chemistry SN - 1477-0520 IS - 9 VL - 7 SP - 1829 EP - 1842 ER -