Discovery - University of Dundee - Online Publications

Library & Learning Centre

E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis

E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis

Research output: Contribution to journalArticle

View graph of relations

Authors

  • Leena Ackermann
  • Michael Schell
  • Wojciech Pokrzywa
  • Éva Kevei
  • Anton Gartner
  • Björn Schumacher (Lead / Corresponding author)
  • Thorsten Hoppe (Lead / Corresponding author)

Research units

Info

Original languageEnglish
Pages (from-to)995-1002
Number of pages8
JournalNature Structural & Molecular Biology
Volume23
Issue number11
Early online date26 Sep 2016
DOIs
StatePublished - Nov 2016

Abstract

Multiple protein ubiquitination events at DNA double-strand breaks (DSBs) regulate damage recognition, signaling and repair. It has remained poorly understood how the repair process of DSBs is coordinated with the apoptotic response. Here, we identified the E4 ubiquitin ligase UFD-2 as a mediator of DNA-damage-induced apoptosis in a genetic screen in Caenorhabditis elegans. We found that, after initiation of homologous recombination by RAD-51, UFD-2 forms foci that contain substrate-processivity factors including the ubiquitin-selective segregase CDC-48 (p97), the deubiquitination enzyme ATX-3 (Ataxin-3) and the proteasome. In the absence of UFD-2, RAD-51 foci persist, and DNA damage-induced apoptosis is prevented. In contrast, UFD-2 foci are retained until recombination intermediates are removed by the Holliday-junction-processing enzymes GEN-1, MUS-81 or XPF-1. Formation of UFD-2 foci also requires proapoptotic CEP-1 (p53) signaling. Our findings establish a central role of UFD-2 in the coordination between the DNA-repair process and the apoptotic response.

Download statistics

No data available

Documents

Open Access permissions

Open

Documents

DOI

Library & Learning Centre

Contact | Accessibility | Policy