Discovery - University of Dundee - Online Publications

Library & Learning Centre

Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum

Standard

Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum. / Chen, Wen-Ming; Prell, Jurgen; James, Euan K.; Sheu, Der-Shyan; Sheu, Shih-Yi.

In: Microbiology-SGM, Vol. 158, 04.2012, p. 1127-1136.

Research output: Contribution to journalArticle

Harvard

Chen, W-M, Prell, J, James, EK, Sheu, D-S & Sheu, S-Y 2012, 'Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum' Microbiology-SGM, vol 158, pp. 1127-1136.

APA

Chen, W-M., Prell, J., James, E. K., Sheu, D-S., & Sheu, S-Y. (2012). Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum. Microbiology-SGM, 158, 1127-1136doi: 10.1099/mic.0.055095-0

Vancouver

Chen W-M, Prell J, James EK, Sheu D-S, Sheu S-Y. Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum. Microbiology-SGM. 2012 Apr;158:1127-1136.

Author

Chen, Wen-Ming; Prell, Jurgen; James, Euan K.; Sheu, Der-Shyan; Sheu, Shih-Yi / Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum.

In: Microbiology-SGM, Vol. 158, 04.2012, p. 1127-1136.

Research output: Contribution to journalArticle

Bibtex - Download

@article{12042b77fb234532beacffad24e7e535,
title = "Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum",
author = "Wen-Ming Chen and Jurgen Prell and James, {Euan K.} and Der-Shyan Sheu and Shih-Yi Sheu",
year = "2012",
volume = "158",
pages = "1127--1136",
journal = "Microbiology-SGM",
issn = "1350-0872",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Effect of phosphoglycerate mutase and fructose 1,6-bisphosphatase deficiency on symbiotic Burkholderia phymatum

A1 - Chen,Wen-Ming

A1 - Prell,Jurgen

A1 - James,Euan K.

A1 - Sheu,Der-Shyan

A1 - Sheu,Shih-Yi

AU - Chen,Wen-Ming

AU - Prell,Jurgen

AU - James,Euan K.

AU - Sheu,Der-Shyan

AU - Sheu,Shih-Yi

PY - 2012/4

Y1 - 2012/4

N2 - <p>Burkholderia phymatum STM815 is a beta-rhizobial strain that can effectively nodulate several species of the large legume genus Mimosa. Two Tn5-induced mutants of this strain, KM16-22 and KM51, failed to form root nodules on Mimosa pudica, but still caused root hair deformation, which is one of the early steps of rhizobial infection. Both mutants grew well in a complex medium. However, KM16-22 could not grow on minimal medium unless a sugar and a metabolic intermediate such as pyruvate were provided, and KM51 also could not grow on minimal medium unless a sugar was added. The Tn5-interrupted genes of the mutants showed strong homologies to pgm, which encodes 2,3-biphosphoglycerate-dependent phosphoglycerate mutase (dPGM), and fbp, which encodes fructose 1,6-bisphosphatase (FBPase). Both enzymes are known to be involved in obligate steps in carbohydrate metabolism. Enzyme assays confirmed that KM16-22 and KM51 had indeed lost dPGM and FBPase activity, respectively, whilst the activities of these enzymes were expressed normally in both free-living bacteria and symbiotic bacteroids of the parental strain STM815. Both mutants recovered their enzyme activity after the introduction of wild-type pgm or fbp genes, were subsequently able to use carbohydrate as a carbon source, and were able to form root nodules on M. pudica and to fix nitrogen as efficiently as the parental strain. We conclude that the enzymes dPGM and FBPase are essential for the formation of a symbiosis with the host plant.</p>

AB - <p>Burkholderia phymatum STM815 is a beta-rhizobial strain that can effectively nodulate several species of the large legume genus Mimosa. Two Tn5-induced mutants of this strain, KM16-22 and KM51, failed to form root nodules on Mimosa pudica, but still caused root hair deformation, which is one of the early steps of rhizobial infection. Both mutants grew well in a complex medium. However, KM16-22 could not grow on minimal medium unless a sugar and a metabolic intermediate such as pyruvate were provided, and KM51 also could not grow on minimal medium unless a sugar was added. The Tn5-interrupted genes of the mutants showed strong homologies to pgm, which encodes 2,3-biphosphoglycerate-dependent phosphoglycerate mutase (dPGM), and fbp, which encodes fructose 1,6-bisphosphatase (FBPase). Both enzymes are known to be involved in obligate steps in carbohydrate metabolism. Enzyme assays confirmed that KM16-22 and KM51 had indeed lost dPGM and FBPase activity, respectively, whilst the activities of these enzymes were expressed normally in both free-living bacteria and symbiotic bacteroids of the parental strain STM815. Both mutants recovered their enzyme activity after the introduction of wild-type pgm or fbp genes, were subsequently able to use carbohydrate as a carbon source, and were able to form root nodules on M. pudica and to fix nitrogen as efficiently as the parental strain. We conclude that the enzymes dPGM and FBPase are essential for the formation of a symbiosis with the host plant.</p>

U2 - 10.1099/mic.0.055095-0

DO - 10.1099/mic.0.055095-0

M1 - Article

JO - Microbiology-SGM

JF - Microbiology-SGM

SN - 1350-0872

VL - 158

SP - 1127

EP - 1136

ER -

Documents

Library & Learning Centre

Contact | Accessibility | Policy