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Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1

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Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1. / Makrantoni, Vasso; Stark, Michael J. R.

In: Molecular and Cellular Biology, Vol. 29, No. 16, 15.08.2009, p. 4552-4562.

Research output: Contribution to journalArticle

Harvard

Makrantoni, V & Stark, MJR 2009, 'Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1' Molecular and Cellular Biology, vol 29, no. 16, pp. 4552-4562.

APA

Makrantoni, V., & Stark, M. J. R. (2009). Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1. Molecular and Cellular Biology, 29(16), 4552-4562doi: 10.1128/MCB.01911-08

Vancouver

Makrantoni V, Stark MJR. Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1. Molecular and Cellular Biology. 2009 Aug 15;29(16):4552-4562.

Author

Makrantoni, Vasso; Stark, Michael J. R. / Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1.

In: Molecular and Cellular Biology, Vol. 29, No. 16, 15.08.2009, p. 4552-4562.

Research output: Contribution to journalArticle

Bibtex - Download

@article{b89c018dd00b4f3ebf2289d5eddff90e,
title = "Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1",
author = "Vasso Makrantoni and Stark, {Michael J. R.}",
year = "2009",
volume = "29",
number = "16",
pages = "4552--4562",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Efficient chromosome biorientation and the tension checkpoint in saccharomyces cerevisiae both require Bir1

A1 - Makrantoni,Vasso

A1 - Stark,Michael J. R.

AU - Makrantoni,Vasso

AU - Stark,Michael J. R.

PY - 2009/8/15

Y1 - 2009/8/15

N2 - <p>Accurate chromosome segregation requires the capture of sister kinetochores by microtubules from opposite spindle poles prior to the initiation of anaphase, a state termed chromosome biorientation. In the budding yeast Saccharomyces cerevisiae, the conserved protein kinase Ipl1 (Aurora B in metazoans) is critical for ensuring correct chromosomal alignment. Ipl1 associates with its activators Sli15 (INCENP), Nbl1 (Borealin), and Bir1 (Survivin), but while Sli15 clearly functions with Ipl1 to promote chromosome biorientation, the role of Bir1 has been uncertain. Using a temperature-sensitive bir1 mutant (bir1-17), we show that Bir1 is needed to permit efficient chromosome biorientation. However, once established, chromosome biorientation is maintained in bir1-17 cells at the restrictive temperature. Ipl1 is partially delocalized in bir1-17 cells, and its protein kinase activity is markedly reduced under nonpermissive conditions. bir1-17 cells arrest normally in response to microtubule depolymerization but fail to delay anaphase when sister kinetochore tension is reduced. Thus, Bir1 is required for the tension checkpoint. Despite their robust mitotic arrest in response to nocodazole, bir1-17 cells are hypersensitive to microtubule-depolymerizing drugs and show a more severe biorientation defect on recovery from nocodazole treatment. The role of Bir1 therefore may become more critical when spindle formation is delayed.</p>

AB - <p>Accurate chromosome segregation requires the capture of sister kinetochores by microtubules from opposite spindle poles prior to the initiation of anaphase, a state termed chromosome biorientation. In the budding yeast Saccharomyces cerevisiae, the conserved protein kinase Ipl1 (Aurora B in metazoans) is critical for ensuring correct chromosomal alignment. Ipl1 associates with its activators Sli15 (INCENP), Nbl1 (Borealin), and Bir1 (Survivin), but while Sli15 clearly functions with Ipl1 to promote chromosome biorientation, the role of Bir1 has been uncertain. Using a temperature-sensitive bir1 mutant (bir1-17), we show that Bir1 is needed to permit efficient chromosome biorientation. However, once established, chromosome biorientation is maintained in bir1-17 cells at the restrictive temperature. Ipl1 is partially delocalized in bir1-17 cells, and its protein kinase activity is markedly reduced under nonpermissive conditions. bir1-17 cells arrest normally in response to microtubule depolymerization but fail to delay anaphase when sister kinetochore tension is reduced. Thus, Bir1 is required for the tension checkpoint. Despite their robust mitotic arrest in response to nocodazole, bir1-17 cells are hypersensitive to microtubule-depolymerizing drugs and show a more severe biorientation defect on recovery from nocodazole treatment. The role of Bir1 therefore may become more critical when spindle formation is delayed.</p>

KW - SPINDLE CHECKPOINT

KW - PROTEIN-KINASE

KW - BUDDING YEAST

KW - MITOTIC SPINDLE

KW - AURORA-B

KW - COMPLEX

KW - PHOSPHORYLATION

KW - ANAPHASE

KW - KINETOCHORES

KW - SEGREGATION

U2 - 10.1128/MCB.01911-08

DO - 10.1128/MCB.01911-08

M1 - Article

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 16

VL - 29

SP - 4552

EP - 4562

ER -

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