Exploring the role of p97 and its UBX-domain cofactors through identification of their interacting proteins. / Alexandru, G.
Methods in Molecular Biology: Ubiquitin Family Modifiers and the Proteasome. Vol. 832 Springer, 2012. p. 305-312.Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
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TY - CHAP
T1 - Exploring the role of p97 and its UBX-domain cofactors through identification of their interacting proteins
A1 - Alexandru,G.
AU - Alexandru,G.
PB - Springer
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The elucidation of protein-protein interaction networks can provide preliminary insights into the function of uncharacterized proteins based on the interactions they establish in the cell. Here, we describe a protein immunoprecipitation protocol that can be used in combination with mass spectrometry analysis to identify the p97 interactome as well as specific subgroups of proteins interacting with its UBX-domain adaptors. This approach aims to dissect the role played by individual UBX cofactors within the complex array of cellular functions performed by p97. © 2012 Springer Science+Business Media, LLC.
AB - The elucidation of protein-protein interaction networks can provide preliminary insights into the function of uncharacterized proteins based on the interactions they establish in the cell. Here, we describe a protein immunoprecipitation protocol that can be used in combination with mass spectrometry analysis to identify the p97 interactome as well as specific subgroups of proteins interacting with its UBX-domain adaptors. This approach aims to dissect the role played by individual UBX cofactors within the complex array of cellular functions performed by p97. © 2012 Springer Science+Business Media, LLC.
UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84858187770&md5=d094f59d47355300d420f6dceaff6692
U2 - 10.1007/978-1-61779-474-2_21
DO - 10.1007/978-1-61779-474-2_21
M1 - Other chapter contribution
SN - 9781617794735
VL - 832
BT - Methods in Molecular Biology
T2 - Methods in Molecular Biology
SP - 305
EP - 312
ER -