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Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants

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Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants. / MacLeod, A. Kenneth; Kelly, Vincent P.; Higgins, Larry G.; Kelleher, Michael O.; Price, Sally A.; Bigley, Alison L.; Betton, Graham R.; Hayes, John D.

In: Drug Metabolism and Disposition, Vol. 38, No. 2, 02.2010, p. 341-346.

Research output: Contribution to journalArticle

Harvard

MacLeod, AK, Kelly, VP, Higgins, LG, Kelleher, MO, Price, SA, Bigley, AL, Betton, GR & Hayes, JD 2010, 'Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants' Drug Metabolism and Disposition, vol 38, no. 2, pp. 341-346., 10.1124/dmd.109.030544

APA

MacLeod, A. K., Kelly, V. P., Higgins, L. G., Kelleher, M. O., Price, S. A., Bigley, A. L., ... Hayes, J. D. (2010). Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants. Drug Metabolism and Disposition, 38(2), 341-346. 10.1124/dmd.109.030544

Vancouver

MacLeod AK, Kelly VP, Higgins LG, Kelleher MO, Price SA, Bigley AL et al. Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants. Drug Metabolism and Disposition. 2010 Feb;38(2):341-346. Available from: 10.1124/dmd.109.030544

Author

MacLeod, A. Kenneth; Kelly, Vincent P.; Higgins, Larry G.; Kelleher, Michael O.; Price, Sally A.; Bigley, Alison L.; Betton, Graham R.; Hayes, John D. / Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants.

In: Drug Metabolism and Disposition, Vol. 38, No. 2, 02.2010, p. 341-346.

Research output: Contribution to journalArticle

Bibtex - Download

@article{91a7b4b802d14162bfe419d5cc03149d,
title = "Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants",
keywords = "GLUTATHIONE-S-TRANSFERASE, ALDEHYDE REDUCTASE, BILE-ACIDS, DIHYDRODIOL DEHYDROGENASE, RESPONSE ELEMENT, GENE, IDENTIFICATION, CHEMOPROTECTORS, PURIFICATION, ACTIVATION",
author = "MacLeod, {A. Kenneth} and Kelly, {Vincent P.} and Higgins, {Larry G.} and Kelleher, {Michael O.} and Price, {Sally A.} and Bigley, {Alison L.} and Betton, {Graham R.} and Hayes, {John D.}",
year = "2010",
doi = "10.1124/dmd.109.030544",
volume = "38",
number = "2",
pages = "341--346",
journal = "Drug Metabolism and Disposition",
issn = "0090-9556",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Expression and Localization of Rat Aldo-Keto Reductases and Induction of the 1B13 and 1D2 Isoforms by Phenolic Antioxidants

A1 - MacLeod,A. Kenneth

A1 - Kelly,Vincent P.

A1 - Higgins,Larry G.

A1 - Kelleher,Michael O.

A1 - Price,Sally A.

A1 - Bigley,Alison L.

A1 - Betton,Graham R.

A1 - Hayes,John D.

AU - MacLeod,A. Kenneth

AU - Kelly,Vincent P.

AU - Higgins,Larry G.

AU - Kelleher,Michael O.

AU - Price,Sally A.

AU - Bigley,Alison L.

AU - Betton,Graham R.

AU - Hayes,John D.

PY - 2010/2

Y1 - 2010/2

N2 - <p>The aldo-keto reductase (AKR) phase I drug metabolism enzyme superfamily is implicated in detoxification or bioactivation of a wide variety of carbonyl-bearing compounds. In this study, we have used antibodies raised against purified recombinant rat AKR isoforms 1A3, 1B4, 1C9, 1D2, and 7A1 to characterize the expression profile of these superfamily members in the rat and define their localization by immunohistochemistry. Western blotting showed that AKR1A3, AKR1B4, and AKR1C9 are ubiquitously expressed, whereas AKR1D2 and AKR7A1 are present in liver, adrenal gland, and kidney, with the latter also present in testis, spleen, and stomach. Immunohistochemical analysis of the kidney demonstrated the localization of AKR1A3 in proximal convoluted tubules, AKR1B4 in the loop of Henle, and AKR1C9 in the pars recta S3 segment of proximal tubules. We also report localization of AKR1B4 in the adrenal gland (parenchymal cells of the zona reticularis) and testis ( Sertoli cells and late spermatids), of AKR1D2 in the liver ( hepatocyte nuclei), and of AKR7A1 in the pancreatic duct and bronchiolar epithelium. Previous studies have shown that expression of AKR7A1 is induced in response to dietary administration of the phenolic antioxidants butylated hydroxyanisole and ethoxyquin. Here we identify AKR1B13 and AKR1D2 as further inducible members of the rat AKR superfamily.</p>

AB - <p>The aldo-keto reductase (AKR) phase I drug metabolism enzyme superfamily is implicated in detoxification or bioactivation of a wide variety of carbonyl-bearing compounds. In this study, we have used antibodies raised against purified recombinant rat AKR isoforms 1A3, 1B4, 1C9, 1D2, and 7A1 to characterize the expression profile of these superfamily members in the rat and define their localization by immunohistochemistry. Western blotting showed that AKR1A3, AKR1B4, and AKR1C9 are ubiquitously expressed, whereas AKR1D2 and AKR7A1 are present in liver, adrenal gland, and kidney, with the latter also present in testis, spleen, and stomach. Immunohistochemical analysis of the kidney demonstrated the localization of AKR1A3 in proximal convoluted tubules, AKR1B4 in the loop of Henle, and AKR1C9 in the pars recta S3 segment of proximal tubules. We also report localization of AKR1B4 in the adrenal gland (parenchymal cells of the zona reticularis) and testis ( Sertoli cells and late spermatids), of AKR1D2 in the liver ( hepatocyte nuclei), and of AKR7A1 in the pancreatic duct and bronchiolar epithelium. Previous studies have shown that expression of AKR7A1 is induced in response to dietary administration of the phenolic antioxidants butylated hydroxyanisole and ethoxyquin. Here we identify AKR1B13 and AKR1D2 as further inducible members of the rat AKR superfamily.</p>

KW - GLUTATHIONE-S-TRANSFERASE

KW - ALDEHYDE REDUCTASE

KW - BILE-ACIDS

KW - DIHYDRODIOL DEHYDROGENASE

KW - RESPONSE ELEMENT

KW - GENE

KW - IDENTIFICATION

KW - CHEMOPROTECTORS

KW - PURIFICATION

KW - ACTIVATION

U2 - 10.1124/dmd.109.030544

DO - 10.1124/dmd.109.030544

M1 - Article

JO - Drug Metabolism and Disposition

JF - Drug Metabolism and Disposition

SN - 0090-9556

IS - 2

VL - 38

SP - 341

EP - 346

ER -

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