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FAF1, a Gene that Is Disrupted in Cleft Palate and Has Conserved Function in Zebrafish

FAF1, a Gene that Is Disrupted in Cleft Palate and Has Conserved Function in Zebrafish

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Authors

  • Michella Ghassibe-Sabbagh
  • Laurence Desmyter
  • Tobias Langenberg
  • Filip Claes
  • Odile Boute
  • Benedicte Bayet
  • Philippe Pellerin
  • Karlien Hermans
  • Liesbeth Backx
  • Maria Adela Mansilla
  • Sandra Imoehl
  • Stefanie Nowak
  • Kerstin U. Ludwig
  • Carlotta Baluardo
  • Melissa Ferrian
  • Markus Noethen
  • Mieke Dewerchin
  • Genevieve Francois
  • Nicole Revencu
  • Romain Vanwijck
  • Jacqueline Hecht
  • Elisabeth Mangold
  • Jeffrey Murray
  • Michele Rubini
  • Joris R. Vermeesch
  • Helene A. Poirel
  • Peter Carmeliet
  • Miikka Vikkula

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Info

Original languageEnglish
Pages150-161
Number of pages12
JournalAmerican Journal of Human Genetics
Journal publication date11 Feb 2011
Volume88
Issue2
DOIs
StatePublished

Abstract

Cranial neural crest (CNC) is a multipotent migratory cell population that gives rise to most of the craniofacial bones. An intricate network mediates CNC formation, epithelial-mesenchymal transition, migration along distinct paths, and differentiation. Errors in these processes lead to craniofacial abnormalities, including cleft lip and palate. Clefts are the most common congenital craniofacial defects. Patients have complications with feeding, speech, hearing, and dental and psychological development. Affected by both genetic predisposition and environmental factors, the complex etiology of clefts remains largely unknown. Here we show that Fas-associated factor-1 (FAF1) is disrupted and that its expression is decreased in a Pierre Robin family with an inherited translocation. Furthermore, the locus is strongly associated with cleft palate and shows an increased relative risk. Expression studies show that faf1 is highly expressed in zebrafish cartilages during embryogenesis. Knockdown of zebrafish faf1 leads to pharyngeal cartilage defects and jaw abnormality as a result of a failure of CNC to differentiate into and express cartilage-specific markers, such as sox9a and col2a1. Administration of faf1 mRNA rescues this phenotype. Our findings therefore identify FAF1 as a regulator of CNC differentiation and show that it predisposes humans to cleft palate and is necessary for lower jaw development in zebrafish.

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