FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk

TY - JOUR

T1 - FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk

A1 - Martinez-Morales,Patricia L.

A1 - Diez del Corral,Ruth

A1 - Olivera-Martinez,Isabel

A1 - Quiroga,Alejandra C.

A1 - Das,Raman M.

A1 - Barbas,Julio A.

A1 - Storey,Kate G.

A1 - Morales,Aixa V.

AU - Martinez-Morales,Patricia L.

AU - Diez del Corral,Ruth

AU - Olivera-Martinez,Isabel

AU - Quiroga,Alejandra C.

AU - Das,Raman M.

AU - Barbas,Julio A.

AU - Storey,Kate G.

AU - Morales,Aixa V.

PY - 2011

Y1 - 2011

N2 - <p>Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control the onset of neurogenesis in the extending neural tube. In this paper, using gain-and loss-of-function experiments, we show that caudal FGF signaling prevents premature specification of NCCs and, consequently, premature epithelial-mesenchymal transition (EMT) to allow cell emigration. In contrast, rostrally generated RA promotes EMT of NCCs at somitic levels. Furthermore, we show that FGF and RA signaling control EMT in part through the modulation of elements of the bone morphogenetic protein and Wnt signaling pathways. These data establish a clear role for opposition of FGF and RA signaling in control of the timing of NCC EMT and emigration and, consequently, co-ordination of the development of the central and peripheral nervous system during vertebrate trunk elongation.</p> <p><strong>© 2011 Martínez-Morales et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/)</strong></p>

AB - <p>Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control the onset of neurogenesis in the extending neural tube. In this paper, using gain-and loss-of-function experiments, we show that caudal FGF signaling prevents premature specification of NCCs and, consequently, premature epithelial-mesenchymal transition (EMT) to allow cell emigration. In contrast, rostrally generated RA promotes EMT of NCCs at somitic levels. Furthermore, we show that FGF and RA signaling control EMT in part through the modulation of elements of the bone morphogenetic protein and Wnt signaling pathways. These data establish a clear role for opposition of FGF and RA signaling in control of the timing of NCC EMT and emigration and, consequently, co-ordination of the development of the central and peripheral nervous system during vertebrate trunk elongation.</p> <p><strong>© 2011 Martínez-Morales et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/)</strong></p>

KW - Epithelial mesenchymal transition

KW - Transcription factor FoxD3

KW - Tyrosine kinase domain

KW - Vertebrate body axis

KW - Neuronal differentiation

KW - Signaling controls

KW - Paraxial mesoderm

KW - Fate restrictions

KW - Opposing FGF

KW - Chick embryo

U2 - 10.1083/jcb.201011077

DO - 10.1083/jcb.201011077

M1 - Article

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

VL - 194

SP - 489

EP - 503

ER -