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Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

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Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. / Karadaglic, D.; Wood, A. D.; McRobbie, M.; Stojanovic, R.; Herrington, C. S.

In: Cancer Epidemiology, Biomarkers & Prevention, Vol. 33, No. 6, 12.2009, p. 463-468.

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Harvard

Karadaglic, D, Wood, AD, McRobbie, M, Stojanovic, R & Herrington, CS 2009, 'Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid' Cancer Epidemiology, Biomarkers & Prevention, vol 33, no. 6, pp. 463-468.

APA

Karadaglic, D., Wood, A. D., McRobbie, M., Stojanovic, R., & Herrington, C. S. (2009). Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. Cancer Epidemiology, Biomarkers & Prevention, 33(6), 463-468doi: 10.1016/j.canep.2009.10.010

Vancouver

Karadaglic D, Wood AD, McRobbie M, Stojanovic R, Herrington CS. Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid. Cancer Epidemiology, Biomarkers & Prevention. 2009 Dec;33(6):463-468.

Author

Karadaglic, D.; Wood, A. D.; McRobbie, M.; Stojanovic, R.; Herrington, C. S. / Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid.

In: Cancer Epidemiology, Biomarkers & Prevention, Vol. 33, No. 6, 12.2009, p. 463-468.

Research output: Contribution to journalArticle

Bibtex - Download

@article{a1d6b2b6997f4721b9df61d814610433,
title = "Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid",
author = "D. Karadaglic and Wood, {A. D.} and M. McRobbie and R. Stojanovic and Herrington, {C. S.}",
year = "2009",
volume = "33",
number = "6",
pages = "463--468",
journal = "Cancer Epidemiology, Biomarkers & Prevention",
issn = "1055-9965",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Fluorescence spectroscopy of an in vitro model of human cervical neoplasia identifies graded spectral shape changes with neoplastic phenotype and a differential effect of acetic acid

A1 - Karadaglic,D.

A1 - Wood,A. D.

A1 - McRobbie,M.

A1 - Stojanovic,R.

A1 - Herrington,C. S.

AU - Karadaglic,D.

AU - Wood,A. D.

AU - McRobbie,M.

AU - Stojanovic,R.

AU - Herrington,C. S.

PY - 2009/12

Y1 - 2009/12

N2 - <p>Background: The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. Methods: in this study, we used organotypic epithelial raft Culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically. The spectrum of cervical dysplasia was modelled by producing rafts lined by primary human keratinocytes (PHKs) and the HaCaT, SiHa and CaSki human keratinocyte cell lines and fluorescence emission spectra were recorded at a wide range of excitation wavelengths. Results: Statistically significant differences in spectral shape were identified between the different rafts at excitation wavelengths between 250 nm and 310 nm. A graded, differential effect of acetic acid on fluorescence intensity was also observed, consistent with the visible effects of acetic acid on clinical examination at colposcopy. Conclusion: These data suggest that the development of neoplastic changes in the squamous epithelium of the cervix are associated with alterations in its fluorescence properties and that the application of acetic acid has a demonstrable effect on these properties. Identification of these alterations may aid the discrimination of cervical lesions in vivo. (C) 2009 Elsevier Ltd. All rights reserved.</p>

AB - <p>Background: The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo. Methods: in this study, we used organotypic epithelial raft Culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically. The spectrum of cervical dysplasia was modelled by producing rafts lined by primary human keratinocytes (PHKs) and the HaCaT, SiHa and CaSki human keratinocyte cell lines and fluorescence emission spectra were recorded at a wide range of excitation wavelengths. Results: Statistically significant differences in spectral shape were identified between the different rafts at excitation wavelengths between 250 nm and 310 nm. A graded, differential effect of acetic acid on fluorescence intensity was also observed, consistent with the visible effects of acetic acid on clinical examination at colposcopy. Conclusion: These data suggest that the development of neoplastic changes in the squamous epithelium of the cervix are associated with alterations in its fluorescence properties and that the application of acetic acid has a demonstrable effect on these properties. Identification of these alterations may aid the discrimination of cervical lesions in vivo. (C) 2009 Elsevier Ltd. All rights reserved.</p>

KW - Cervical neoplasia

KW - Fluorescence spectroscopy

KW - Acetic acid

KW - Epithelial raft culture

KW - EXCITATION WAVELENGTHS

KW - CELL-LINES

KW - TISSUE

KW - TETRASOMY

KW - CARCINOMA

KW - CULTURES

KW - PROTEIN

KW - GROWTH

KW - CASKI

KW - SIHA

U2 - 10.1016/j.canep.2009.10.010

DO - 10.1016/j.canep.2009.10.010

M1 - Article

JO - Cancer Epidemiology, Biomarkers & Prevention

JF - Cancer Epidemiology, Biomarkers & Prevention

SN - 1055-9965

IS - 6

VL - 33

SP - 463

EP - 468

ER -

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