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Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

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Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. / Praveen, Bavishna B.; Ashok, Praveen C.; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan.

In: Journal of Biomedical Optics, Vol. 17, No. 7, 077006, 07.2012, p. -.

Research output: Contribution to journalArticle

Harvard

Praveen, BB, Ashok, PC, Mazilu, M, Riches, A, Herrington, S & Dholakia, K 2012, 'Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis' Journal of Biomedical Optics, vol 17, no. 7, 077006, pp. -., 10.1117/1.JBO.17.7.077006

APA

Praveen, B. B., Ashok, P. C., Mazilu, M., Riches, A., Herrington, S., & Dholakia, K. (2012). Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. Journal of Biomedical Optics, 17(7), -. [077006]. 10.1117/1.JBO.17.7.077006

Vancouver

Praveen BB, Ashok PC, Mazilu M, Riches A, Herrington S, Dholakia K. Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. Journal of Biomedical Optics. 2012 Jul;17(7):-. 077006. Available from: 10.1117/1.JBO.17.7.077006

Author

Praveen, Bavishna B.; Ashok, Praveen C.; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan / Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis.

In: Journal of Biomedical Optics, Vol. 17, No. 7, 077006, 07.2012, p. -.

Research output: Contribution to journalArticle

Bibtex - Download

@article{e31e51e4f6ec49d0a4cf4c4b88f1d07f,
title = "Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis",
author = "Praveen, {Bavishna B.} and Ashok, {Praveen C.} and Michael Mazilu and Andrew Riches and Simon Herrington and Kishan Dholakia",
year = "2012",
doi = "10.1117/1.JBO.17.7.077006",
volume = "17",
number = "7",
pages = "--",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

A1 - Praveen,Bavishna B.

A1 - Ashok,Praveen C.

A1 - Mazilu,Michael

A1 - Riches,Andrew

A1 - Herrington,Simon

A1 - Dholakia,Kishan

AU - Praveen,Bavishna B.

AU - Ashok,Praveen C.

AU - Mazilu,Michael

AU - Riches,Andrew

AU - Herrington,Simon

AU - Dholakia,Kishan

PY - 2012/7

Y1 - 2012/7

N2 - <p>In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (similar to 30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.077006]</p>

AB - <p>In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (similar to 30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.077006]</p>

UR - http://www.scopus.com/inward/record.url?scp=84872031905&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.17.7.077006

DO - 10.1117/1.JBO.17.7.077006

M1 - Article

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 7

VL - 17

SP - -

ER -

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