Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. / Praveen, Bavishna B.; Ashok, Praveen C.; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan.
In: Journal of Biomedical Optics, Vol. 17, No. 7, 07.2012, p. -, 077006.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis
A1 - Praveen,Bavishna B.
A1 - Ashok,Praveen C.
A1 - Mazilu,Michael
A1 - Riches,Andrew
A1 - Herrington,Simon
A1 - Dholakia,Kishan
AU - Praveen,Bavishna B.
AU - Ashok,Praveen C.
AU - Mazilu,Michael
AU - Riches,Andrew
AU - Herrington,Simon
AU - Dholakia,Kishan
PY - 2012/7
Y1 - 2012/7
N2 - <p>In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (similar to 30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.077006]</p>
AB - <p>In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (similar to 30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.077006]</p>
U2 - 10.1117/1.JBO.17.7.077006
DO - 10.1117/1.JBO.17.7.077006
M1 - Article
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 7
VL - 17
SP - -
ER -