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Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

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Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
EditorsTuan Vo-Dinh, Warren S. Grundfest, Anita Mahadevan-Jansen
Place of PublicationBellingham
PublisherSPIE-International Society for Optical Engineering
ISBN (Print)9780819479518
StatePublished - 2010

Publication series

NameProceedings of SPIE


ConferenceSPIE Photonics West 2010: Advanced Biomedical and Clinical Diagnostic Systems VIII
CountryUnited States
CitySan Francisco
Internet address


The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.



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