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Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

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    Original languageEnglish
    TitleAdvanced biomedical and clinical diagnostic systems VIII
    EditorsT VoDinh, WS Grundfest, A MahadevanJansen
    Place of publicationBellingham
    PublisherSPIE-International Society for Optical Engineering
    Publication date2010
    Pages-
    Number of pages6
    ISBN (Print)978-0-8194-7951-8
    DOIs
    StatePublished

    Conference

    ConferenceSPIE Photonics West 2010: Conference on Advanced Biomedical and Clinical Diagnostic Systems VIII
    CitySan Francisco
    Period23/01/1028/01/10
    Internet addresshttp://spie.org/x39144.xml

    Abstract

    The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.

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