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Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Authors

  • Anna Chiara De Luca
  • Michael Mazilu
  • Andrew Riches
  • Simon Herrington
  • Kishan Dholakia

Research units

    Info

    Original languageEnglish
    Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
    EditorsTuan Vo-Dinh, Warren S. Grundfest, Anita Mahadevan-Jansen
    Place of publicationBellingham
    PublisherSPIE-International Society for Optical Engineering
    Publication date2010
    ISBN (Print)9780819479518
    DOIs
    StatePublished

    Publication series

    NameProceedings of SPIE
    PublisherSPIE
    Volume7555

    Conference

    ConferenceSPIE Photonics West 2010: Advanced Biomedical and Clinical Diagnostic Systems VIII
    CountryUnited States
    CitySan Francisco
    Period24/01/1026/01/10
    Internet addresshttp://spie.org/x39144.xml

    Abstract

    The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.

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