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Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

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Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy. / De Luca, Anna Chiara; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan.

Advanced biomedical and clinical diagnostic systems VIII. ed. / T VoDinh; WS Grundfest; A MahadevanJansen. Bellingham : SPIE-International Society for Optical Engineering, 2010. p. -.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harvard

De Luca, AC, Mazilu, M, Riches, A, Herrington, S & Dholakia, K 2010, 'Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy'. in T VoDinh, WS Grundfest & A MahadevanJansen (eds), Advanced biomedical and clinical diagnostic systems VIII. SPIE-International Society for Optical Engineering, Bellingham, pp. -, SPIE Photonics West 2010: Conference on Advanced Biomedical and Clinical Diagnostic Systems VIII, San Francisco, 23-28 January.

APA

De Luca, A. C., Mazilu, M., Riches, A., Herrington, S., & Dholakia, K. (2010). Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy. In VoDinh, T., Grundfest, W. S., & MahadevanJansen, A. (Eds.), Advanced biomedical and clinical diagnostic systems VIII. (pp. -). Bellingham: SPIE-International Society for Optical Engineering. doi: 10.1117/12.841686

Vancouver

De Luca AC, Mazilu M, Riches A, Herrington S, Dholakia K. Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy. In VoDinh T, Grundfest WS, MahadevanJansen A, editors, Advanced biomedical and clinical diagnostic systems VIII. Bellingham: SPIE-International Society for Optical Engineering. 2010. p. -.

Author

De Luca, Anna Chiara; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan / Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy.

Advanced biomedical and clinical diagnostic systems VIII. ed. / T VoDinh; WS Grundfest; A MahadevanJansen. Bellingham : SPIE-International Society for Optical Engineering, 2010. p. -.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bibtex - Download

@inbook{5594dd513a2d4ef4a419bd8e89f48540,
title = "Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy",
publisher = "SPIE-International Society for Optical Engineering",
author = "{De Luca}, {Anna Chiara} and Michael Mazilu and Andrew Riches and Simon Herrington and Kishan Dholakia",
year = "2010",
editor = "T VoDinh and WS Grundfest and A MahadevanJansen",
isbn = "978-0-8194-7951-8",
pages = "-",
booktitle = "Advanced biomedical and clinical diagnostic systems VIII",

}

RIS (suitable for import to EndNote) - Download

TY - CHAP

T1 - Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

A1 - De Luca,Anna Chiara

A1 - Mazilu,Michael

A1 - Riches,Andrew

A1 - Herrington,Simon

A1 - Dholakia,Kishan

AU - De Luca,Anna Chiara

AU - Mazilu,Michael

AU - Riches,Andrew

AU - Herrington,Simon

AU - Dholakia,Kishan

PB - SPIE-International Society for Optical Engineering

CY - Bellingham

PY - 2010

Y1 - 2010

N2 - <p>The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.</p>

AB - <p>The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.</p>

KW - Raman spectroscopy

KW - fluorescence-free

KW - PCA

KW - REJECTION

KW - TWEEZERS

U2 - 10.1117/12.841686

DO - 10.1117/12.841686

M1 - Conference contribution

SN - 978-0-8194-7951-8

BT - Advanced biomedical and clinical diagnostic systems VIII

T2 - Advanced biomedical and clinical diagnostic systems VIII

A2 - MahadevanJansen,A

ED - MahadevanJansen,A

SP - -

ER -

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