Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy. / De Luca, Anna Chiara; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan.
Advanced biomedical and clinical diagnostic systems VIII. ed. / T VoDinh; WS Grundfest; A MahadevanJansen. Bellingham : SPIE-International Society for Optical Engineering, 2010. p. -.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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TY - CHAP
T1 - Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy
A1 - De Luca,Anna Chiara
A1 - Mazilu,Michael
A1 - Riches,Andrew
A1 - Herrington,Simon
A1 - Dholakia,Kishan
AU - De Luca,Anna Chiara
AU - Mazilu,Michael
AU - Riches,Andrew
AU - Herrington,Simon
AU - Dholakia,Kishan
PB - SPIE-International Society for Optical Engineering
CY - Bellingham
PY - 2010
Y1 - 2010
N2 - <p>The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.</p>
AB - <p>The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.</p>
KW - Raman spectroscopy
KW - fluorescence-free
KW - PCA
KW - REJECTION
KW - TWEEZERS
U2 - 10.1117/12.841686
DO - 10.1117/12.841686
M1 - Conference contribution
SN - 978-0-8194-7951-8
BT - Advanced biomedical and clinical diagnostic systems VIII
T2 - Advanced biomedical and clinical diagnostic systems VIII
A2 - MahadevanJansen,A
ED - MahadevanJansen,A
SP - -
ER -