Discovery - University of Dundee - Online Publications

Library & Learning Centre

HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma

Standard

HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma. / Purdie, Colin A.; Jordan, Lee B.; McCullough, Jean B.; Edwards, Sharon L.; Cunningham, Joan; Walsh, Miriam; Grant, Andrew; Pratt, Norman; Thompson, Alastair M.

In: Histopathology, Vol. 56, No. 6, 05.2010, p. 702-707.

Research output: Contribution to journalArticle

Harvard

Purdie, CA, Jordan, LB, McCullough, JB, Edwards, SL, Cunningham, J, Walsh, M, Grant, A, Pratt, N & Thompson, AM 2010, 'HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma' Histopathology, vol 56, no. 6, pp. 702-707., 10.1111/j.1365-2559.2010.03533.x

APA

Purdie, C. A., Jordan, L. B., McCullough, J. B., Edwards, S. L., Cunningham, J., Walsh, M., ... Thompson, A. M. (2010). HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma. Histopathology, 56(6), 702-707. 10.1111/j.1365-2559.2010.03533.x

Vancouver

Purdie CA, Jordan LB, McCullough JB, Edwards SL, Cunningham J, Walsh M et al. HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma. Histopathology. 2010 May;56(6):702-707. Available from: 10.1111/j.1365-2559.2010.03533.x

Author

Purdie, Colin A.; Jordan, Lee B.; McCullough, Jean B.; Edwards, Sharon L.; Cunningham, Joan; Walsh, Miriam; Grant, Andrew; Pratt, Norman; Thompson, Alastair M. / HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma.

In: Histopathology, Vol. 56, No. 6, 05.2010, p. 702-707.

Research output: Contribution to journalArticle

Bibtex - Download

@article{87ff4f58423e4ea2acd25be292ec8e4f,
title = "HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma",
keywords = "breast cancer, CB11, core biopsy, HER2 FISH, HER2 immunohistochemistry, IN-SITU HYBRIDIZATION, ADJUVANT CHEMOTHERAPY, GENE AMPLIFICATION, ESTROGEN-RECEPTOR, CANCER, TRASTUZUMAB, HER-2/NEU, ONCOGENE, IMMUNOHISTOCHEMISTRY, EXPRESSION",
author = "Purdie, {Colin A.} and Jordan, {Lee B.} and McCullough, {Jean B.} and Edwards, {Sharon L.} and Joan Cunningham and Miriam Walsh and Andrew Grant and Norman Pratt and Thompson, {Alastair M.}",
year = "2010",
doi = "10.1111/j.1365-2559.2010.03533.x",
volume = "56",
number = "6",
pages = "702--707",
journal = "Histopathology",
issn = "0309-0167",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma

A1 - Purdie,Colin A.

A1 - Jordan,Lee B.

A1 - McCullough,Jean B.

A1 - Edwards,Sharon L.

A1 - Cunningham,Joan

A1 - Walsh,Miriam

A1 - Grant,Andrew

A1 - Pratt,Norman

A1 - Thompson,Alastair M.

AU - Purdie,Colin A.

AU - Jordan,Lee B.

AU - McCullough,Jean B.

AU - Edwards,Sharon L.

AU - Cunningham,Joan

AU - Walsh,Miriam

AU - Grant,Andrew

AU - Pratt,Norman

AU - Thompson,Alastair M.

PY - 2010/5

Y1 - 2010/5

N2 - <p>Aims:</p><p>HER2 status is a prognostic factor in breast carcinoma and predicts response to trastuzumab therapy. Trastuzumab is effective in the neoadjuvant, adjuvant and advanced disease settings. Knowledge of HER2 status early in the diagnostic and therapeutic process is vital for treatment planning. HER2 analysis is usually carried out by immunohistochemistry or fluorescence in situ hybridization (FISH). The aim of this study was to establish whether HER2 immunohistochemistry using monoclonal antibody CB11 and carried out on the initial diagnostic core biopsy specimen accurately predicts HER2 amplification status.</p><p>Methods and results:</p><p>This is the largest study to date in which HER2 protein expression has been assessed by CB11 immunohistochemistry on the diagnostic core biopsy specimen and correlated with the result of FISH. Using FISH as the definitive HER2 status, we studied 568 invasive breast cancers using CB11 immunohistochemistry on core biopsy. This analysis had a sensitivity of 99.4%, specificity of 93.9%, false-positive frequency of 3.9% and false-negative frequency of 1.1%. These data are as good as those obtained from analysing resection specimens alone in UK national reference centres.</p><p>Conclusions:</p><p>CB11 immunohistochemistry accurately predicts HER2 amplification status and can be reliably carried out on core biopsy specimens of breast carcinoma.</p>

AB - <p>Aims:</p><p>HER2 status is a prognostic factor in breast carcinoma and predicts response to trastuzumab therapy. Trastuzumab is effective in the neoadjuvant, adjuvant and advanced disease settings. Knowledge of HER2 status early in the diagnostic and therapeutic process is vital for treatment planning. HER2 analysis is usually carried out by immunohistochemistry or fluorescence in situ hybridization (FISH). The aim of this study was to establish whether HER2 immunohistochemistry using monoclonal antibody CB11 and carried out on the initial diagnostic core biopsy specimen accurately predicts HER2 amplification status.</p><p>Methods and results:</p><p>This is the largest study to date in which HER2 protein expression has been assessed by CB11 immunohistochemistry on the diagnostic core biopsy specimen and correlated with the result of FISH. Using FISH as the definitive HER2 status, we studied 568 invasive breast cancers using CB11 immunohistochemistry on core biopsy. This analysis had a sensitivity of 99.4%, specificity of 93.9%, false-positive frequency of 3.9% and false-negative frequency of 1.1%. These data are as good as those obtained from analysing resection specimens alone in UK national reference centres.</p><p>Conclusions:</p><p>CB11 immunohistochemistry accurately predicts HER2 amplification status and can be reliably carried out on core biopsy specimens of breast carcinoma.</p>

KW - breast cancer

KW - CB11

KW - core biopsy

KW - HER2 FISH

KW - HER2 immunohistochemistry

KW - IN-SITU HYBRIDIZATION

KW - ADJUVANT CHEMOTHERAPY

KW - GENE AMPLIFICATION

KW - ESTROGEN-RECEPTOR

KW - CANCER

KW - TRASTUZUMAB

KW - HER-2/NEU

KW - ONCOGENE

KW - IMMUNOHISTOCHEMISTRY

KW - EXPRESSION

U2 - 10.1111/j.1365-2559.2010.03533.x

DO - 10.1111/j.1365-2559.2010.03533.x

M1 - Article

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 6

VL - 56

SP - 702

EP - 707

ER -

Documents

Library & Learning Centre

Contact | Accessibility | Policy