Discovery - University of Dundee - Online Publications

Library & Learning Centre

Histone tails and the H3 alphaN helix regulate nucleosome mobility and stability

Histone tails and the H3 alphaN helix regulate nucleosome mobility and stability

Research output: Contribution to journalArticle

View graph of relations

Authors

  • Helder Ferreira
  • Joanna Somers
  • Ryan Webster
  • Andrew Flaus
  • Tom Owen-Hughes

Research units

Info

Original languageEnglish
Pages4037-48
Number of pages12
JournalMolecular and Cellular Biology
Journal publication date2007
Journal number11
Volume27
DOIs
StatePublished

Abstract

Nucleosomes fulfill the apparently conflicting roles of compacting DNA within eukaryotic genomes while permitting access to regulatory factors. Central to this is their ability to stably associate with DNA while retaining the ability to undergo rearrangements that increase access to the underlying DNA. Here, we have studied different aspects of nucleosome dynamics including nucleosome sliding, histone dimer exchange, and DNA wrapping within nucleosomes. We find that alterations to histone proteins, especially the histone tails and vicinity of the histone H3 alphaN helix, can affect these processes differently, suggesting that they are mechanistically distinct. This raises the possibility that modifications to histone proteins may provide a means of fine-tuning specific aspects of the dynamic properties of nucleosomes to the context in which they are located.

Documents

Library & Learning Centre

Contact | Accessibility | Policy