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Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

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Authors

  • Stephan Wawra
  • Judith Bain
  • Elaine Durward
  • Irene de Bruijn
  • Kirsty L. Minor
  • Anja Matena
  • Lars Löbach
  • Stephen C. Whisson
  • Peter Bayer
  • Andrew J. Porter
  • Paul R. J. Birch
  • Chris J. Secombes
  • Pieter van West

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Info

Original languageEnglish
Pages2096-2101
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Journal publication date7 Feb 2012
Volume109
Issue6
DOIs
StatePublished

Abstract

The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.

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