Discovery - University of Dundee - Online Publications

Library & Learning Centre

Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

Standard

Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. / Wawra, Stephan; Bain, Judith; Durward, Elaine; de Bruijn, Irene; Minor, Kirsty L.; Matena, Anja; Loebach, Lars; Whisson, Stephen C.; Bayer, Peter; Porter, Andrew J.; Birch, Paul R. J.; Secombes, Chris J.; van West, Pieter.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 6, 07.02.2012, p. 2096-2101.

Research output: Contribution to journalArticle

Harvard

Wawra, S, Bain, J, Durward, E, de Bruijn, I, Minor, KL, Matena, A, Loebach, L, Whisson, SC, Bayer, P, Porter, AJ, Birch, PRJ, Secombes, CJ & van West, P 2012, 'Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner' Proceedings of the National Academy of Sciences of the United States of America, vol 109, no. 6, pp. 2096-2101.

APA

Wawra, S., Bain, J., Durward, E., de Bruijn, I., Minor, K. L., Matena, A., Loebach, L., Whisson, S. C., Bayer, P., Porter, A. J., Birch, P. R. J., Secombes, C. J., & van West, P. (2012). Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. Proceedings of the National Academy of Sciences of the United States of America, 109(6), 2096-2101doi: 10.1073/pnas.1113775109

Vancouver

Wawra S, Bain J, Durward E, de Bruijn I, Minor KL, Matena A et al. Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. Proceedings of the National Academy of Sciences of the United States of America. 2012 Feb 7;109(6):2096-2101.

Author

Wawra, Stephan; Bain, Judith; Durward, Elaine; de Bruijn, Irene; Minor, Kirsty L.; Matena, Anja; Loebach, Lars; Whisson, Stephen C.; Bayer, Peter; Porter, Andrew J.; Birch, Paul R. J.; Secombes, Chris J.; van West, Pieter / Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 6, 07.02.2012, p. 2096-2101.

Research output: Contribution to journalArticle

Bibtex - Download

@article{0aa11fc1c1b54bca92a73f873b220d34,
title = "Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner",
author = "Stephan Wawra and Judith Bain and Elaine Durward and {de Bruijn}, Irene and Minor, {Kirsty L.} and Anja Matena and Lars Loebach and Whisson, {Stephen C.} and Peter Bayer and Porter, {Andrew J.} and Birch, {Paul R. J.} and Secombes, {Chris J.} and {van West}, Pieter",
year = "2012",
volume = "109",
number = "6",
pages = "2096--2101",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

A1 - Wawra,Stephan

A1 - Bain,Judith

A1 - Durward,Elaine

A1 - de Bruijn,Irene

A1 - Minor,Kirsty L.

A1 - Matena,Anja

A1 - Loebach,Lars

A1 - Whisson,Stephen C.

A1 - Bayer,Peter

A1 - Porter,Andrew J.

A1 - Birch,Paul R. J.

A1 - Secombes,Chris J.

A1 - van West,Pieter

AU - Wawra,Stephan

AU - Bain,Judith

AU - Durward,Elaine

AU - de Bruijn,Irene

AU - Minor,Kirsty L.

AU - Matena,Anja

AU - Loebach,Lars

AU - Whisson,Stephen C.

AU - Bayer,Peter

AU - Porter,Andrew J.

AU - Birch,Paul R. J.

AU - Secombes,Chris J.

AU - van West,Pieter

PY - 2012/2/7

Y1 - 2012/2/7

N2 - <p>The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.</p>

AB - <p>The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.</p>

KW - protein translocation

KW - Phytophthora

KW - Plasmodium

KW - PATHOGEN PHYTOPHTHORA-INFESTANS

KW - SOJAE-EFFECTOR AVR1B

KW - PLANT-CELLS

KW - ERYTHROCYTE

KW - SECRETION

KW - VIRULENCE

KW - BINDING

KW - GENOME

KW - ENTRY

KW - AVIRULENCE

U2 - 10.1073/pnas.1113775109

DO - 10.1073/pnas.1113775109

M1 - Article

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

VL - 109

SP - 2096

EP - 2101

ER -

Documents

Library & Learning Centre

Contact | Accessibility | Policy