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Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

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Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. / Wawra, Stephan; Bain, Judith; Durward, Elaine; de Bruijn, Irene; Minor, Kirsty L.; Matena, Anja; Löbach, Lars; Whisson, Stephen C.; Bayer, Peter; Porter, Andrew J.; Birch, Paul R. J.; Secombes, Chris J.; van West, Pieter.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 6, 07.02.2012, p. 2096-2101.

Research output: Contribution to journalArticle

Harvard

Wawra, S, Bain, J, Durward, E, de Bruijn, I, Minor, KL, Matena, A, Löbach, L, Whisson, SC, Bayer, P, Porter, AJ, Birch, PRJ, Secombes, CJ & van West, P 2012, 'Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner' Proceedings of the National Academy of Sciences of the United States of America, vol 109, no. 6, pp. 2096-2101., 10.1073/pnas.1113775109

APA

Wawra, S., Bain, J., Durward, E., de Bruijn, I., Minor, K. L., Matena, A., ... van West, P. (2012). Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. Proceedings of the National Academy of Sciences of the United States of America, 109(6), 2096-2101. 10.1073/pnas.1113775109

Vancouver

Wawra S, Bain J, Durward E, de Bruijn I, Minor KL, Matena A et al. Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. Proceedings of the National Academy of Sciences of the United States of America. 2012 Feb 7;109(6):2096-2101. Available from: 10.1073/pnas.1113775109

Author

Wawra, Stephan; Bain, Judith; Durward, Elaine; de Bruijn, Irene; Minor, Kirsty L.; Matena, Anja; Löbach, Lars; Whisson, Stephen C.; Bayer, Peter; Porter, Andrew J.; Birch, Paul R. J.; Secombes, Chris J.; van West, Pieter / Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 6, 07.02.2012, p. 2096-2101.

Research output: Contribution to journalArticle

Bibtex - Download

@article{0aa11fc1c1b54bca92a73f873b220d34,
title = "Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner",
keywords = "protein translocation, Phytophthora, Plasmodium, PATHOGEN PHYTOPHTHORA-INFESTANS, SOJAE-EFFECTOR AVR1B, PLANT-CELLS, ERYTHROCYTE, SECRETION, VIRULENCE, BINDING, GENOME, ENTRY, AVIRULENCE",
author = "Stephan Wawra and Judith Bain and Elaine Durward and {de Bruijn}, Irene and Minor, {Kirsty L.} and Anja Matena and Lars Löbach and Whisson, {Stephen C.} and Peter Bayer and Porter, {Andrew J.} and Birch, {Paul R. J.} and Secombes, {Chris J.} and {van West}, Pieter",
year = "2012",
doi = "10.1073/pnas.1113775109",
volume = "109",
number = "6",
pages = "2096--2101",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

A1 - Wawra,Stephan

A1 - Bain,Judith

A1 - Durward,Elaine

A1 - de Bruijn,Irene

A1 - Minor,Kirsty L.

A1 - Matena,Anja

A1 - Löbach,Lars

A1 - Whisson,Stephen C.

A1 - Bayer,Peter

A1 - Porter,Andrew J.

A1 - Birch,Paul R. J.

A1 - Secombes,Chris J.

A1 - van West,Pieter

AU - Wawra,Stephan

AU - Bain,Judith

AU - Durward,Elaine

AU - de Bruijn,Irene

AU - Minor,Kirsty L.

AU - Matena,Anja

AU - Löbach,Lars

AU - Whisson,Stephen C.

AU - Bayer,Peter

AU - Porter,Andrew J.

AU - Birch,Paul R. J.

AU - Secombes,Chris J.

AU - van West,Pieter

PY - 2012/2/7

Y1 - 2012/2/7

N2 - <p>The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.</p>

AB - <p>The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.</p>

KW - protein translocation

KW - Phytophthora

KW - Plasmodium

KW - PATHOGEN PHYTOPHTHORA-INFESTANS

KW - SOJAE-EFFECTOR AVR1B

KW - PLANT-CELLS

KW - ERYTHROCYTE

KW - SECRETION

KW - VIRULENCE

KW - BINDING

KW - GENOME

KW - ENTRY

KW - AVIRULENCE

U2 - 10.1073/pnas.1113775109

DO - 10.1073/pnas.1113775109

M1 - Article

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

VL - 109

SP - 2096

EP - 2101

ER -

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