Discovery - University of Dundee - Online Publications

Library & Learning Centre

Identification of Bacillus subtilis SipW as a Bifunctional Signal Peptidase That Controls Surface-Adhered Biofilm Formation

Identification of Bacillus subtilis SipW as a Bifunctional Signal Peptidase That Controls Surface-Adhered Biofilm Formation

Research output: Contribution to journalArticle

View graph of relations

Authors

Research units

Info

Original languageEnglish
Pages2781-2790
Number of pages10
JournalJournal of Bacteriology
Journal publication dateJun 2012
Volume194
Issue11
DOIs
StatePublished
Peer-reviewedYes

Abstract

Biofilms of microbial cells encased in an exopolymeric matrix can form on solid surfaces, but how bacteria sense a solid surface and upregulate biofilm genes is largely unknown. We investigated the role of the Bacillus subtilis signal peptidase, SipW, which has a unique role in forming biofilms on a solid surface and is not required at an air-liquid interface. Surprisingly, we found that the signal peptidase activity of SipW was not required for solid-surface biofilms. Furthermore, a SipW mutant protein was constructed that lacks the ability to form a solid-surface biofilm but still retains signal peptidase activity. Through genetic and gene expression tests, the non-signal peptidase role of SipW was found to activate biofilm matrix genes specifically when cells were on a solid surface. These data provide the first evidence that a signal peptidase is bifunctional and that SipW has a regulatory role in addition to its role as a signal peptidase.

Documents

Library & Learning Centre

Contact | Accessibility | Policy