Identification of Bacillus subtilis SipW as a Bifunctional Signal Peptidase That Controls Surface-Adhered Biofilm Formation. / Terra, Rebecca; Stanley-Wall, Nicola R.; Cao, Guoqiang; Lazazzera, Beth A.
In: Journal of Bacteriology, Vol. 194, No. 11, 06.2012, p. 2781-2790.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of <em>Bacillus subtilis </em>SipW as a Bifunctional Signal Peptidase That Controls Surface-Adhered Biofilm Formation
A1 - Terra,Rebecca
A1 - Stanley-Wall,Nicola R.
A1 - Cao,Guoqiang
A1 - Lazazzera,Beth A.
AU - Terra,Rebecca
AU - Stanley-Wall,Nicola R.
AU - Cao,Guoqiang
AU - Lazazzera,Beth A.
PY - 2012/6
Y1 - 2012/6
N2 - <p>Biofilms of microbial cells encased in an exopolymeric matrix can form on solid surfaces, but how bacteria sense a solid surface and upregulate biofilm genes is largely unknown. We investigated the role of the Bacillus subtilis signal peptidase, SipW, which has a unique role in forming biofilms on a solid surface and is not required at an air-liquid interface. Surprisingly, we found that the signal peptidase activity of SipW was not required for solid-surface biofilms. Furthermore, a SipW mutant protein was constructed that lacks the ability to form a solid-surface biofilm but still retains signal peptidase activity. Through genetic and gene expression tests, the non-signal peptidase role of SipW was found to activate biofilm matrix genes specifically when cells were on a solid surface. These data provide the first evidence that a signal peptidase is bifunctional and that SipW has a regulatory role in addition to its role as a signal peptidase.</p>
AB - <p>Biofilms of microbial cells encased in an exopolymeric matrix can form on solid surfaces, but how bacteria sense a solid surface and upregulate biofilm genes is largely unknown. We investigated the role of the Bacillus subtilis signal peptidase, SipW, which has a unique role in forming biofilms on a solid surface and is not required at an air-liquid interface. Surprisingly, we found that the signal peptidase activity of SipW was not required for solid-surface biofilms. Furthermore, a SipW mutant protein was constructed that lacks the ability to form a solid-surface biofilm but still retains signal peptidase activity. Through genetic and gene expression tests, the non-signal peptidase role of SipW was found to activate biofilm matrix genes specifically when cells were on a solid surface. These data provide the first evidence that a signal peptidase is bifunctional and that SipW has a regulatory role in addition to its role as a signal peptidase.</p>
U2 - 10.1128/JB.06780-11
DO - 10.1128/JB.06780-11
M1 - Article
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 11
VL - 194
SP - 2781
EP - 2790
ER -