Identification of human miRNA precursors that resemble box C/D snoRNAs. / Ono, Motoharu; Scott, Michelle S.; Yamada, Kayo; Avolio, Fabio; Barton, Geoffrey J.; Lamond, Angus I.
In: Nucleic Acids Research, Vol. 39, No. 9, 05.2011, p. 3879-3891.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of human miRNA precursors that resemble box C/D snoRNAs
AU - Ono,Motoharu
AU - Scott,Michelle S.
AU - Yamada,Kayo
AU - Avolio,Fabio
AU - Barton,Geoffrey J.
AU - Lamond,Angus I.
PY - 2011/5
Y1 - 2011/5
N2 - There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.
AB - There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.
KW - RNA SECONDARY STRUCTURE
KW - SMALL NUCLEOLAR RNAS
KW - HOST GENES
KW - MICRORNAS
KW - NUCLEAR
KW - CANDIDATES
KW - DYNAMICS
KW - REVEALS
KW - MOUSE
KW - CELLS
U2 - 10.1093/nar/gkq1355
DO - 10.1093/nar/gkq1355
M3 - Article
VL - 39
SP - 3879
EP - 3891
JO - Nucleic Acids Research
T2 - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 9
ER -