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Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo

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Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo. / Paranjape, Sachin A.; Chan, Owen; Zhu, Wanling; Horblitt, Adam M.; McNay, Ewan C.; Cresswell, James A.; Bogan, Jonathan S.; McCrimmon, Rory J.; Sherwin, Robert S.

In: Diabetes, Vol. 59, No. 6, 06.2010, p. 1521-1527.

Research output: Contribution to journalArticle

Harvard

Paranjape, SA, Chan, O, Zhu, W, Horblitt, AM, McNay, EC, Cresswell, JA, Bogan, JS, McCrimmon, RJ & Sherwin, RS 2010, 'Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo' Diabetes, vol 59, no. 6, pp. 1521-1527., 10.2337/db10-0014

APA

Paranjape, S. A., Chan, O., Zhu, W., Horblitt, A. M., McNay, E. C., Cresswell, J. A., ... Sherwin, R. S. (2010). Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo. Diabetes, 59(6), 1521-1527. 10.2337/db10-0014

Vancouver

Paranjape SA, Chan O, Zhu W, Horblitt AM, McNay EC, Cresswell JA et al. Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo. Diabetes. 2010 Jun;59(6):1521-1527. Available from: 10.2337/db10-0014

Author

Paranjape, Sachin A.; Chan, Owen; Zhu, Wanling; Horblitt, Adam M.; McNay, Ewan C.; Cresswell, James A.; Bogan, Jonathan S.; McCrimmon, Rory J.; Sherwin, Robert S. / Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo.

In: Diabetes, Vol. 59, No. 6, 06.2010, p. 1521-1527.

Research output: Contribution to journalArticle

Bibtex - Download

@article{4ce25dd7867c435d8c52dba07690beca,
title = "Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo",
keywords = "COUNTERREGULATORY HORMONE RESPONSES, CENTRAL-NERVOUS-SYSTEM, CIRCULATING INSULIN, GLUCOSE-PRODUCTION, CONSCIOUS DOG, ALPHA-CELLS, HYPOGLYCEMIA, RECEPTOR, NEURONS, BRAIN",
author = "Paranjape, {Sachin A.} and Owen Chan and Wanling Zhu and Horblitt, {Adam M.} and McNay, {Ewan C.} and Cresswell, {James A.} and Bogan, {Jonathan S.} and McCrimmon, {Rory J.} and Sherwin, {Robert S.}",
year = "2010",
doi = "10.2337/db10-0014",
volume = "59",
number = "6",
pages = "1521--1527",
journal = "Diabetes",
issn = "0012-1797",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Influence of Insulin in the Ventromedial Hypothalamus on Pancreatic Glucagon Secretion In Vivo

A1 - Paranjape,Sachin A.

A1 - Chan,Owen

A1 - Zhu,Wanling

A1 - Horblitt,Adam M.

A1 - McNay,Ewan C.

A1 - Cresswell,James A.

A1 - Bogan,Jonathan S.

A1 - McCrimmon,Rory J.

A1 - Sherwin,Robert S.

AU - Paranjape,Sachin A.

AU - Chan,Owen

AU - Zhu,Wanling

AU - Horblitt,Adam M.

AU - McNay,Ewan C.

AU - Cresswell,James A.

AU - Bogan,Jonathan S.

AU - McCrimmon,Rory J.

AU - Sherwin,Robert S.

PY - 2010/6

Y1 - 2010/6

N2 - <p>OBJECTIVE-Insulin released by the beta-cell is thought to act locally to regulate glucagon secretion. The possibility that insulin might also act centrally to modulate islet glucagon secretion has received little attention. RESEARCH</p><p>DESIGN AND METHODS-Initially the counter-regulatory response to identical hypoglycemia was compared during intravenous insulin and phloridzin infusion in awake chronically catheterized nondiabetic rats. To explore whether the disparate glucagon responses seen were in part due to changes in ventromedial hypothalamus (VMH) exposure to insulin, bilateral guide cannulas were inserted to the level of the VMH and 8 days later rats received a VMH microinjection of either 1) anti-insulin affibody, 2) control affibody, 3) artificial extracellular fluid, 4) insulin (50 mu U), 5) insulin receptor antagonist (S961), or 6) anti-insulin affibody plus a gamma-aminobutyric acid A (GABA(A)) receptor agonist muscimol, prior to a hypoglycemic clamp or under baseline conditions.</p><p>RESULTS-As expected, insulin-induced hypoglycemia produced a threefold increase in plasma glucagon. However, the glucagon response was fourfold to fivefold greater when circulating insulin did not increase, despite equivalent hypoglycemia and C-peptide suppression. In contrast, epinephrine responses were not altered. The phloridzin-hypoglycemia induced glucagon increase was attenuated (40%) by VMH insulin microinjection. Conversely, local VMH blockade of insulin amplified glucagon twofold to threefold during insulin-induced hypoglycemia. Furthermore, local blockade of basal insulin levels or insulin receptors within the VMH caused an immediate twofold increase in fasting glucagon levels that was prevented by coinjection to the VMH of a GABA(A) receptor agonist.</p><p>CONCLUSIONS-Together, these data suggest that insulin's inhibitory effect on alpha-cell glucagon release is in part mediated at the level of the VMH under both nomoglycemic and hypoglycemic conditions. Diabetes 59:1521-1527, 2010</p>

AB - <p>OBJECTIVE-Insulin released by the beta-cell is thought to act locally to regulate glucagon secretion. The possibility that insulin might also act centrally to modulate islet glucagon secretion has received little attention. RESEARCH</p><p>DESIGN AND METHODS-Initially the counter-regulatory response to identical hypoglycemia was compared during intravenous insulin and phloridzin infusion in awake chronically catheterized nondiabetic rats. To explore whether the disparate glucagon responses seen were in part due to changes in ventromedial hypothalamus (VMH) exposure to insulin, bilateral guide cannulas were inserted to the level of the VMH and 8 days later rats received a VMH microinjection of either 1) anti-insulin affibody, 2) control affibody, 3) artificial extracellular fluid, 4) insulin (50 mu U), 5) insulin receptor antagonist (S961), or 6) anti-insulin affibody plus a gamma-aminobutyric acid A (GABA(A)) receptor agonist muscimol, prior to a hypoglycemic clamp or under baseline conditions.</p><p>RESULTS-As expected, insulin-induced hypoglycemia produced a threefold increase in plasma glucagon. However, the glucagon response was fourfold to fivefold greater when circulating insulin did not increase, despite equivalent hypoglycemia and C-peptide suppression. In contrast, epinephrine responses were not altered. The phloridzin-hypoglycemia induced glucagon increase was attenuated (40%) by VMH insulin microinjection. Conversely, local VMH blockade of insulin amplified glucagon twofold to threefold during insulin-induced hypoglycemia. Furthermore, local blockade of basal insulin levels or insulin receptors within the VMH caused an immediate twofold increase in fasting glucagon levels that was prevented by coinjection to the VMH of a GABA(A) receptor agonist.</p><p>CONCLUSIONS-Together, these data suggest that insulin's inhibitory effect on alpha-cell glucagon release is in part mediated at the level of the VMH under both nomoglycemic and hypoglycemic conditions. Diabetes 59:1521-1527, 2010</p>

KW - COUNTERREGULATORY HORMONE RESPONSES

KW - CENTRAL-NERVOUS-SYSTEM

KW - CIRCULATING INSULIN

KW - GLUCOSE-PRODUCTION

KW - CONSCIOUS DOG

KW - ALPHA-CELLS

KW - HYPOGLYCEMIA

KW - RECEPTOR

KW - NEURONS

KW - BRAIN

U2 - 10.2337/db10-0014

DO - 10.2337/db10-0014

M1 - Article

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 6

VL - 59

SP - 1521

EP - 1527

ER -

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