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Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma

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Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma. / Watt, Stephen; Pourreyron, C.; Purdie, K.; Hogan, C.; Cole, C. L.; Foster, N.; Pratt, N.; Bourdon, Jean-Christophe; Appleyard, V.; Murray, K.; Thompson, Alastair; Mao, X.; Mein, C.; Bruckner-Tuderman, L.; Evans, A.; McGrath, J. A.; Proby, C. M.; Foerster, J.; Leigh, Irene; South, Andrew (Lead / Corresponding author).

In: Oncogene, Vol. 30, No. 46, 11.2011, p. 4666-4677.

Research output: Contribution to journalArticle

Harvard

Watt, S, Pourreyron, C, Purdie, K, Hogan, C, Cole, CL, Foster, N, Pratt, N, Bourdon, J-C, Appleyard, V, Murray, K, Thompson, A, Mao, X, Mein, C, Bruckner-Tuderman, L, Evans, A, McGrath, JA, Proby, CM, Foerster, J, Leigh, I & South, A 2011, 'Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma' Oncogene, vol 30, no. 46, pp. 4666-4677.

APA

Watt, S., Pourreyron, C., Purdie, K., Hogan, C., Cole, C. L., Foster, N., Pratt, N., Bourdon, J-C., Appleyard, V., Murray, K., Thompson, A., Mao, X., Mein, C., Bruckner-Tuderman, L., Evans, A., McGrath, J. A., Proby, C. M., Foerster, J., Leigh, I., & South, A. (2011). Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma. Oncogene, 30(46), 4666-4677doi: 10.1038/onc.2011.180

Vancouver

Watt S, Pourreyron C, Purdie K, Hogan C, Cole CL, Foster N et al. Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma. Oncogene. 2011 Nov;30(46):4666-4677.

Author

Watt, Stephen; Pourreyron, C.; Purdie, K.; Hogan, C.; Cole, C. L.; Foster, N.; Pratt, N.; Bourdon, Jean-Christophe; Appleyard, V.; Murray, K.; Thompson, Alastair; Mao, X.; Mein, C.; Bruckner-Tuderman, L.; Evans, A.; McGrath, J. A.; Proby, C. M.; Foerster, J.; Leigh, Irene; South, Andrew (Lead / Corresponding author) / Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma.

In: Oncogene, Vol. 30, No. 46, 11.2011, p. 4666-4677.

Research output: Contribution to journalArticle

Bibtex - Download

@article{663db41a1d9b4942921455dfdc3bc2ed,
title = "Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma",
author = "Stephen Watt and C. Pourreyron and K. Purdie and C. Hogan and Cole, {C. L.} and N. Foster and N. Pratt and Jean-Christophe Bourdon and V. Appleyard and K. Murray and Alastair Thompson and X. Mao and C. Mein and L. Bruckner-Tuderman and A. Evans and McGrath, {J. A.} and Proby, {C. M.} and J. Foerster and Irene Leigh and Andrew South",
note = "MEDLINE® is the source for the MeSH terms of this document.",
year = "2011",
volume = "30",
number = "46",
pages = "4666--4677",
journal = "Oncogene",
issn = "0950-9232",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma

A1 - Watt,Stephen

A1 - Pourreyron,C.

A1 - Purdie,K.

A1 - Hogan,C.

A1 - Cole,C. L.

A1 - Foster,N.

A1 - Pratt,N.

A1 - Bourdon,Jean-Christophe

A1 - Appleyard,V.

A1 - Murray,K.

A1 - Thompson,Alastair

A1 - Mao,X.

A1 - Mein,C.

A1 - Bruckner-Tuderman,L.

A1 - Evans,A.

A1 - McGrath,J. A.

A1 - Proby,C. M.

A1 - Foerster,J.

A1 - Leigh,Irene

A1 - South,Andrew

AU - Watt,Stephen

AU - Pourreyron,C.

AU - Purdie,K.

AU - Hogan,C.

AU - Cole,C. L.

AU - Foster,N.

AU - Pratt,N.

AU - Bourdon,Jean-Christophe

AU - Appleyard,V.

AU - Murray,K.

AU - Thompson,Alastair

AU - Mao,X.

AU - Mein,C.

AU - Bruckner-Tuderman,L.

AU - Evans,A.

AU - McGrath,J. A.

AU - Proby,C. M.

AU - Foerster,J.

AU - Leigh,Irene

AU - South,Andrew

PY - 2011/11

Y1 - 2011/11

N2 - Identifying therapeutic targets for cancer treatment relies on consistent changes within particular types or sub-types of malignancy. The ability to define either consistent changes or sub-types of malignancy is often masked by tumor heterogeneity. To elucidate therapeutic targets in cutaneous squamous cell carcinoma (cSCC), the most frequent skin neoplasm with malignant potential, we have developed an integrated approach to gene expression profiling beginning with primary keratinocytes in culture. Candidate drivers of cSCC development were derived by first defining a set of in vitro cancer genes and then comparing their expression in a range of clinical data sets containing normal skin, cSCC and the benign hyper-proliferative condition psoriasis. A small interfering RNA (siRNA) screen of the resulting 21 upregulated genes has yielded targets capable of reducing xenograft tumor volume in vivo. Small-molecule inhibitors for one target, Polo-like kinase-1 (PLK1), are already in clinical trials for other malignancies, and our data show efficacy in cSCC. Another target, C20orf20, is identified as being overexpressed in cSCC, and siRNA-mediated knockdown induces apoptosis in vitro and reduces tumor growth in vivo. Thus, our approach has shown established and uncharacterized drivers of tumorigenesis with potent efficacy as therapeutic targets for the treatment of cSCC. © 2011 Macmillan Publishers Limited All rights reserved.

AB - Identifying therapeutic targets for cancer treatment relies on consistent changes within particular types or sub-types of malignancy. The ability to define either consistent changes or sub-types of malignancy is often masked by tumor heterogeneity. To elucidate therapeutic targets in cutaneous squamous cell carcinoma (cSCC), the most frequent skin neoplasm with malignant potential, we have developed an integrated approach to gene expression profiling beginning with primary keratinocytes in culture. Candidate drivers of cSCC development were derived by first defining a set of in vitro cancer genes and then comparing their expression in a range of clinical data sets containing normal skin, cSCC and the benign hyper-proliferative condition psoriasis. A small interfering RNA (siRNA) screen of the resulting 21 upregulated genes has yielded targets capable of reducing xenograft tumor volume in vivo. Small-molecule inhibitors for one target, Polo-like kinase-1 (PLK1), are already in clinical trials for other malignancies, and our data show efficacy in cSCC. Another target, C20orf20, is identified as being overexpressed in cSCC, and siRNA-mediated knockdown induces apoptosis in vitro and reduces tumor growth in vivo. Thus, our approach has shown established and uncharacterized drivers of tumorigenesis with potent efficacy as therapeutic targets for the treatment of cSCC. © 2011 Macmillan Publishers Limited All rights reserved.

KW - cutaneous squamous cell carcinoma

KW - primary cell culture

KW - gene expression analysis

KW - therapeutic targets

KW - PLK1

KW - POLO-LIKE KINASE

KW - GENE-EXPRESSION PATTERNS

KW - CHRONIC MYELOID-LEUKEMIA

KW - NONMELANOMA SKIN-CANCER

KW - EPITHELIAL-CELLS

KW - ANALYSIS DEFINES

KW - BREAST-CANCER

KW - IN-VIVO

KW - LINES

KW - IDENTIFICATION

UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-81255137880&md5=cfce8535e22dae741eefca80b56e282e

U2 - 10.1038/onc.2011.180

DO - 10.1038/onc.2011.180

M1 - Article

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 46

VL - 30

SP - 4666

EP - 4677

ER -

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