Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. / Jaqaman, Khuloud; King, Emma M.; Amaro, Ana C.; Winter, Jennifer R.; Dorn, Jonas F.; Elliott, Hunter L.; Mchedlishvili, Nunu; McClelland, Sarah E.; Porter, Iain M.; Posch, Markus; Toso, Alberto; Danuser, Gaudenz; McAinsh, Andrew D.; Meraldi, Patrick; Swedlow, Jason R.
In: Journal of Cell Biology, Vol. 188, No. 5, 08.03.2010, p. 665-679.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
A1 - Jaqaman,Khuloud
A1 - King,Emma M.
A1 - Amaro,Ana C.
A1 - Winter,Jennifer R.
A1 - Dorn,Jonas F.
A1 - Elliott,Hunter L.
A1 - Mchedlishvili,Nunu
A1 - McClelland,Sarah E.
A1 - Porter,Iain M.
A1 - Posch,Markus
A1 - Toso,Alberto
A1 - Danuser,Gaudenz
A1 - McAinsh,Andrew D.
A1 - Meraldi,Patrick
A1 - Swedlow,Jason R.
AU - Jaqaman,Khuloud
AU - King,Emma M.
AU - Amaro,Ana C.
AU - Winter,Jennifer R.
AU - Dorn,Jonas F.
AU - Elliott,Hunter L.
AU - Mchedlishvili,Nunu
AU - McClelland,Sarah E.
AU - Porter,Iain M.
AU - Posch,Markus
AU - Toso,Alberto
AU - Danuser,Gaudenz
AU - McAinsh,Andrew D.
AU - Meraldi,Patrick
AU - Swedlow,Jason R.
PY - 2010/3/8
Y1 - 2010/3/8
N2 - <p>During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.</p>
AB - <p>During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.</p>
KW - SPINDLE-ASSEMBLY CHECKPOINT
KW - CHROMOSOME ALIGNMENT
KW - MITOTIC CHROMOSOME
KW - HUMAN-CELLS
KW - CENP-E
KW - MCAK
KW - ANAPHASE
KW - COHESIN
KW - CONGRESSION
KW - ATTACHMENT
U2 - 10.1083/jcb.200909005
DO - 10.1083/jcb.200909005
M1 - Article
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 5
VL - 188
SP - 665
EP - 679
ER -