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Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases

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Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. / Jaqaman, Khuloud; King, Emma M.; Amaro, Ana C.; Winter, Jennifer R.; Dorn, Jonas F.; Elliott, Hunter L.; Mchedlishvili, Nunu; McClelland, Sarah E.; Porter, Iain M.; Posch, Markus; Toso, Alberto; Danuser, Gaudenz (Lead / Corresponding author); McAinsh, Andrew D. (Lead / Corresponding author); Meraldi, Patrick (Lead / Corresponding author); Swedlow, Jason R. (Lead / Corresponding author).

In: Journal of Cell Biology, Vol. 188, No. 5, 08.03.2010, p. 665-679.

Research output: Contribution to journalArticle

Harvard

Jaqaman, K, King, EM, Amaro, AC, Winter, JR, Dorn, JF, Elliott, HL, Mchedlishvili, N, McClelland, SE, Porter, IM, Posch, M, Toso, A, Danuser, G, McAinsh, AD, Meraldi, P & Swedlow, JR 2010, 'Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases' Journal of Cell Biology, vol 188, no. 5, pp. 665-679.

APA

Jaqaman, K., King, E. M., Amaro, A. C., Winter, J. R., Dorn, J. F., Elliott, H. L., Mchedlishvili, N., McClelland, S. E., Porter, I. M., Posch, M., Toso, A., Danuser, G., McAinsh, A. D., Meraldi, P., & Swedlow, J. R. (2010). Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. Journal of Cell Biology, 188(5), 665-679doi: 10.1083/jcb.200909005

Vancouver

Jaqaman K, King EM, Amaro AC, Winter JR, Dorn JF, Elliott HL et al. Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. Journal of Cell Biology. 2010 Mar 8;188(5):665-679.

Author

Jaqaman, Khuloud; King, Emma M.; Amaro, Ana C.; Winter, Jennifer R.; Dorn, Jonas F.; Elliott, Hunter L.; Mchedlishvili, Nunu; McClelland, Sarah E.; Porter, Iain M.; Posch, Markus; Toso, Alberto; Danuser, Gaudenz (Lead / Corresponding author); McAinsh, Andrew D. (Lead / Corresponding author); Meraldi, Patrick (Lead / Corresponding author); Swedlow, Jason R. (Lead / Corresponding author) / Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases.

In: Journal of Cell Biology, Vol. 188, No. 5, 08.03.2010, p. 665-679.

Research output: Contribution to journalArticle

Bibtex - Download

@article{6ff4e915236b42d8a652158752c88faa,
title = "Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases",
author = "Khuloud Jaqaman and King, {Emma M.} and Amaro, {Ana C.} and Winter, {Jennifer R.} and Dorn, {Jonas F.} and Elliott, {Hunter L.} and Nunu Mchedlishvili and McClelland, {Sarah E.} and Porter, {Iain M.} and Markus Posch and Alberto Toso and Gaudenz Danuser and McAinsh, {Andrew D.} and Patrick Meraldi and Swedlow, {Jason R.}",
year = "2010",
volume = "188",
number = "5",
pages = "665--679",
journal = "Journal of Cell Biology",
issn = "0021-9525",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases

A1 - Jaqaman,Khuloud

A1 - King,Emma M.

A1 - Amaro,Ana C.

A1 - Winter,Jennifer R.

A1 - Dorn,Jonas F.

A1 - Elliott,Hunter L.

A1 - Mchedlishvili,Nunu

A1 - McClelland,Sarah E.

A1 - Porter,Iain M.

A1 - Posch,Markus

A1 - Toso,Alberto

A1 - Danuser,Gaudenz

A1 - McAinsh,Andrew D.

A1 - Meraldi,Patrick

A1 - Swedlow,Jason R.

AU - Jaqaman,Khuloud

AU - King,Emma M.

AU - Amaro,Ana C.

AU - Winter,Jennifer R.

AU - Dorn,Jonas F.

AU - Elliott,Hunter L.

AU - Mchedlishvili,Nunu

AU - McClelland,Sarah E.

AU - Porter,Iain M.

AU - Posch,Markus

AU - Toso,Alberto

AU - Danuser,Gaudenz

AU - McAinsh,Andrew D.

AU - Meraldi,Patrick

AU - Swedlow,Jason R.

PY - 2010/3/8

Y1 - 2010/3/8

N2 - <p>During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.</p>

AB - <p>During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.</p>

KW - SPINDLE-ASSEMBLY CHECKPOINT

KW - CHROMOSOME ALIGNMENT

KW - MITOTIC CHROMOSOME

KW - HUMAN-CELLS

KW - CENP-E

KW - MCAK

KW - ANAPHASE

KW - COHESIN

KW - CONGRESSION

KW - ATTACHMENT

U2 - 10.1083/jcb.200909005

DO - 10.1083/jcb.200909005

M1 - Article

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

VL - 188

SP - 665

EP - 679

ER -

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