Discovery - University of Dundee - Online Publications

Library & Learning Centre

Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation

Standard

Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation. / Muramoto, Tetsuya; Cannon, Danielle; Gierlinski, Marek; Corrigan, Adam; Barton, Geoffrey J.; Chubb, Jonathan R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 19, 2012, p. 7350-7355.

Research output: Contribution to journalArticle

Harvard

Muramoto, T, Cannon, D, Gierlinski, M, Corrigan, A, Barton, GJ & Chubb, JR 2012, 'Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation' Proceedings of the National Academy of Sciences of the United States of America, vol 109, no. 19, pp. 7350-7355., 10.1073/pnas.1117603109

APA

Muramoto, T., Cannon, D., Gierlinski, M., Corrigan, A., Barton, G. J., & Chubb, J. R. (2012). Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation. Proceedings of the National Academy of Sciences of the United States of America, 109(19), 7350-7355. 10.1073/pnas.1117603109

Vancouver

Muramoto T, Cannon D, Gierlinski M, Corrigan A, Barton GJ, Chubb JR. Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation. Proceedings of the National Academy of Sciences of the United States of America. 2012;109(19):7350-7355. Available from: 10.1073/pnas.1117603109

Author

Muramoto, Tetsuya; Cannon, Danielle; Gierlinski, Marek; Corrigan, Adam; Barton, Geoffrey J.; Chubb, Jonathan R. / Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 19, 2012, p. 7350-7355.

Research output: Contribution to journalArticle

Bibtex - Download

@article{318da84222694adabb79ccd5b4758f5f,
title = "Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation",
author = "Tetsuya Muramoto and Danielle Cannon and Marek Gierlinski and Adam Corrigan and Barton, {Geoffrey J.} and Chubb, {Jonathan R.}",
year = "2012",
doi = "10.1073/pnas.1117603109",
volume = "109",
number = "19",
pages = "7350--7355",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation

A1 - Muramoto,Tetsuya

A1 - Cannon,Danielle

A1 - Gierlinski,Marek

A1 - Corrigan,Adam

A1 - Barton,Geoffrey J.

A1 - Chubb,Jonathan R.

AU - Muramoto,Tetsuya

AU - Cannon,Danielle

AU - Gierlinski,Marek

AU - Corrigan,Adam

AU - Barton,Geoffrey J.

AU - Chubb,Jonathan R.

PY - 2012

Y1 - 2012

N2 - <p>Transcription of genes can be discontinuous, occurring in pulses or bursts. It is not clear how properties of transcriptional pulses vary between different genes. We compared the pulsing of five housekeeping and five developmentally induced genes by direct imaging of single gene transcriptional events in individual living Dictyostelium cells. Each gene displayed its own transcriptional signature, differing in probability of firing and pulse duration, frequency, and intensity. In contrast to the prevailing view from both prokaryotes and eukaryotes that transcription displays binary behavior, strongly expressed housekeeping genes altered the magnitude of their transcriptional pulses during development. These nonbinary "tunable" responses may be better suited than stochastic switch behavior for housekeeping functions. Analysis of RNA synthesis kinetics using fluorescence recovery after photobleaching implied modulation of housekeeping-gene pulse strength occurs at the level of transcription initiation rather than elongation. In addition, disparities between single cell and population measures of transcript production suggested differences in RNA stability between gene classes. Analysis of stability using RNAseq revealed no major global differences in stability between developmental and housekeeping transcripts, although strongly induced RNAs showed unusually rapid decay, indicating tight regulation of expression.</p>

AB - <p>Transcription of genes can be discontinuous, occurring in pulses or bursts. It is not clear how properties of transcriptional pulses vary between different genes. We compared the pulsing of five housekeeping and five developmentally induced genes by direct imaging of single gene transcriptional events in individual living Dictyostelium cells. Each gene displayed its own transcriptional signature, differing in probability of firing and pulse duration, frequency, and intensity. In contrast to the prevailing view from both prokaryotes and eukaryotes that transcription displays binary behavior, strongly expressed housekeeping genes altered the magnitude of their transcriptional pulses during development. These nonbinary "tunable" responses may be better suited than stochastic switch behavior for housekeeping functions. Analysis of RNA synthesis kinetics using fluorescence recovery after photobleaching implied modulation of housekeeping-gene pulse strength occurs at the level of transcription initiation rather than elongation. In addition, disparities between single cell and population measures of transcript production suggested differences in RNA stability between gene classes. Analysis of stability using RNAseq revealed no major global differences in stability between developmental and housekeeping transcripts, although strongly induced RNAs showed unusually rapid decay, indicating tight regulation of expression.</p>

U2 - 10.1073/pnas.1117603109

DO - 10.1073/pnas.1117603109

M1 - Article

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 19

VL - 109

SP - 7350

EP - 7355

ER -

Documents

Library & Learning Centre

Contact | Accessibility | Policy