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Mechanism of Hypoxia-Induced NF-kappa B

Mechanism of Hypoxia-Induced NF-kappa B

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Authors

  • Carolyn Culver
  • Anders Sundqvist
  • Sharon Mudie
  • Andrew Melvin
  • Dimitris Xirodimas
  • Sonia Rocha (Lead / Corresponding author)

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Info

Original languageEnglish
Pages4901-4921
Number of pages21
JournalMolecular and Cellular Biology
Journal publication date10 Oct 2010
Volume30
Issue20
DOIs
StatePublished

Abstract

NF-kappa B activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, I kappa B kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2)dependent pathway distinct from that for other common inducers of NF-kappa B. This process still requires IKK and the IKK kinase TAK1, like that for inflammatory inducers of NF-kappa B, but the TAK1-associated proteins TAB1 and TAB2 are not essential. IKK complex activation following hypoxia requires Ubc13 but not the recently identified LUBAC (linear ubiquitin chain assembly complex) ubiquitin conjugation system. In contrast to the action of other NF-kappa B inducers, IKK-mediated phosphorylation of I kappa B alpha does not result in its degradation. We show that this results from I kappa B alpha sumoylation by Sumo-2/3 on critical lysine residues, normally required for K-48-linked polyubiquitination. Furthermore, inhibition of specific Sumo proteases is sufficient to release RelA from I kappa B alpha and activate NF-kappa B target genes. These results define a novel pathway regulating NF-kappa Bactivation, important to its physiological role in human health and disease.

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