Methylation of H3K4 is required for inheritance of active transcriptional states

TY - JOUR

T1 - Methylation of H3K4 is required for inheritance of active transcriptional states

A1 - Muramoto,Tetsuya

A1 - Mueller,Iris

A1 - Thomas,Giles

A1 - Melvin,Andrew

A1 - Chubb,Jonathan R.

AU - Muramoto,Tetsuya

AU - Mueller,Iris

AU - Thomas,Giles

AU - Melvin,Andrew

AU - Chubb,Jonathan R.

PY - 2010/3/9

Y1 - 2010/3/9

N2 - <p>Background: Maintenance of differentiation programs requires stability, when appropriate, of transcriptional states. However, the extent to which inheritance of active transcriptional states occurs from mother to daughter cells has not been directly addressed in unperturbed cell populations. Results: By live imaging of single-gene transcriptional events in individual cells, we have directly recorded the potential for mitotic inheritance of transcriptional states down cell lineages. Our data showed strong similarity in frequency of transcriptional firing between mother and daughter cells. This memory persisted for complete cell cycles. Both transcriptional pulse length and pulsing rate contributed to overall inheritance, and memory was determined by lineage, not cell environment. Analysis of transcription in chromatin mutants demonstrated that the histone H3K4 methylase Set1 and Ash2, a component of the methylase complex, are required for memory. The effects of Set1 methylation may be mediated directly by chromatin, because loss of memory also occurred when endogenous H3K4 was replaced by alanine. Although methylated H3K4 is usually associated with active transcriptional units, the modification was not required for gene activity but stabilized transcriptional frequency between generations. Conclusions: Our data indicate that methylated H3K4 can act as a chromatin mark reflecting the original meaning of "epigenetic."</p>

AB - <p>Background: Maintenance of differentiation programs requires stability, when appropriate, of transcriptional states. However, the extent to which inheritance of active transcriptional states occurs from mother to daughter cells has not been directly addressed in unperturbed cell populations. Results: By live imaging of single-gene transcriptional events in individual cells, we have directly recorded the potential for mitotic inheritance of transcriptional states down cell lineages. Our data showed strong similarity in frequency of transcriptional firing between mother and daughter cells. This memory persisted for complete cell cycles. Both transcriptional pulse length and pulsing rate contributed to overall inheritance, and memory was determined by lineage, not cell environment. Analysis of transcription in chromatin mutants demonstrated that the histone H3K4 methylase Set1 and Ash2, a component of the methylase complex, are required for memory. The effects of Set1 methylation may be mediated directly by chromatin, because loss of memory also occurred when endogenous H3K4 was replaced by alanine. Although methylated H3K4 is usually associated with active transcriptional units, the modification was not required for gene activity but stabilized transcriptional frequency between generations. Conclusions: Our data indicate that methylated H3K4 can act as a chromatin mark reflecting the original meaning of "epigenetic."</p>

KW - RNA polymerase II

KW - Histone H3

KW - Epigenetic memory

KW - Cell cycle

KW - Dictyostelium-discoideum

KW - DNA methylation

KW - PHD finger

KW - Chromatin

KW - Trimethylation

KW - GENE

U2 - 10.1016/j.cub.2010.01.017

DO - 10.1016/j.cub.2010.01.017

M1 - Article

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 5

VL - 20

SP - 397

EP - 406

ER -