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Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas

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Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas. / Palmieri, C.; Rudraraju, B.; Monteverde, M.; Lattanzio, L.; Gojis, O.; Brizio, R.; Garrone, O.; Merlano, M.; Syed, N.; Lo Nigro, C.; Crook, T.

In: British Journal of Cancer, Vol. 107, No. 2, 10.07.2012, p. 375-381.

Research output: Contribution to journalArticle

Harvard

Palmieri, C, Rudraraju, B, Monteverde, M, Lattanzio, L, Gojis, O, Brizio, R, Garrone, O, Merlano, M, Syed, N, Lo Nigro, C & Crook, T 2012, 'Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas' British Journal of Cancer, vol 107, no. 2, pp. 375-381., 10.1038/bjc.2012.231

APA

Palmieri, C., Rudraraju, B., Monteverde, M., Lattanzio, L., Gojis, O., Brizio, R., ... Crook, T. (2012). Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas. British Journal of Cancer, 107(2), 375-381. 10.1038/bjc.2012.231

Vancouver

Palmieri C, Rudraraju B, Monteverde M, Lattanzio L, Gojis O, Brizio R et al. Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas. British Journal of Cancer. 2012 Jul 10;107(2):375-381. Available from: 10.1038/bjc.2012.231

Author

Palmieri, C.; Rudraraju, B.; Monteverde, M.; Lattanzio, L.; Gojis, O.; Brizio, R.; Garrone, O.; Merlano, M.; Syed, N.; Lo Nigro, C.; Crook, T. / Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas.

In: British Journal of Cancer, Vol. 107, No. 2, 10.07.2012, p. 375-381.

Research output: Contribution to journalArticle

Bibtex - Download

@article{873e9f19168547d0a906f8cbb75e633f,
title = "Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas",
author = "C. Palmieri and B. Rudraraju and M. Monteverde and L. Lattanzio and O. Gojis and R. Brizio and O. Garrone and M. Merlano and N. Syed and {Lo Nigro}, C. and T. Crook",
year = "2012",
doi = "10.1038/bjc.2012.231",
volume = "107",
number = "2",
pages = "375--381",
journal = "British Journal of Cancer",
issn = "0007-0920",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas

A1 - Palmieri,C.

A1 - Rudraraju,B.

A1 - Monteverde,M.

A1 - Lattanzio,L.

A1 - Gojis,O.

A1 - Brizio,R.

A1 - Garrone,O.

A1 - Merlano,M.

A1 - Syed,N.

A1 - Lo Nigro,C.

A1 - Crook,T.

AU - Palmieri,C.

AU - Rudraraju,B.

AU - Monteverde,M.

AU - Lattanzio,L.

AU - Gojis,O.

AU - Brizio,R.

AU - Garrone,O.

AU - Merlano,M.

AU - Syed,N.

AU - Lo Nigro,C.

AU - Crook,T.

PY - 2012/7/10

Y1 - 2012/7/10

N2 - <p>BACKGROUND: Calcium is an important intracellular messenger that mediates many biological processes that are relevant to the malignant process. Calcium ion channels are key in controlling the intracellular calcium, and little is known about their role in human cancer.</p><p>METHODS: We used qPCR and pyrosequencing to investigate expression and epigenetic regulation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) in breast cancer cell lines, primary cancers and metastatic lesions.</p><p>RESULTS: Expression of CACNA2D3 mRNA is regulated in breast cancer cell lines by methylation in the CpG island located in the 50 regulatory region of the gene. Expression is upregulated by azacytidine (AZA) in cells with CpG island methylation but unaffected in cells lacking methylation. In primary breast carcinomas, methylation is more common in cancers, which subsequently relapse with loco-regional and, particularly, visceral metastatic disease in both oestrogen receptor-alpha (ER)-positive and -negative cases. Furthermore, CACNA2D3 CpG island is frequently methylated in breast cancer that has metastasised to the central nervous system.</p><p>CONCLUSION: Methylation-dependent transcriptional silencing of CACNA2D3 may contribute to the metastatic phenotype of breast cancer. Analysis of methylation in the CACNA2D3 CpG island may have potential as a biomarker for risk of development of metastatic disease. British Journal of Cancer (2012) 107, 375-381. doi:10.1038/bjc.2012.231 www.bjcancer.com Published online 29 May 2012 (c) 2012 Cancer Research UK</p>

AB - <p>BACKGROUND: Calcium is an important intracellular messenger that mediates many biological processes that are relevant to the malignant process. Calcium ion channels are key in controlling the intracellular calcium, and little is known about their role in human cancer.</p><p>METHODS: We used qPCR and pyrosequencing to investigate expression and epigenetic regulation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) in breast cancer cell lines, primary cancers and metastatic lesions.</p><p>RESULTS: Expression of CACNA2D3 mRNA is regulated in breast cancer cell lines by methylation in the CpG island located in the 50 regulatory region of the gene. Expression is upregulated by azacytidine (AZA) in cells with CpG island methylation but unaffected in cells lacking methylation. In primary breast carcinomas, methylation is more common in cancers, which subsequently relapse with loco-regional and, particularly, visceral metastatic disease in both oestrogen receptor-alpha (ER)-positive and -negative cases. Furthermore, CACNA2D3 CpG island is frequently methylated in breast cancer that has metastasised to the central nervous system.</p><p>CONCLUSION: Methylation-dependent transcriptional silencing of CACNA2D3 may contribute to the metastatic phenotype of breast cancer. Analysis of methylation in the CACNA2D3 CpG island may have potential as a biomarker for risk of development of metastatic disease. British Journal of Cancer (2012) 107, 375-381. doi:10.1038/bjc.2012.231 www.bjcancer.com Published online 29 May 2012 (c) 2012 Cancer Research UK</p>

U2 - 10.1038/bjc.2012.231

DO - 10.1038/bjc.2012.231

M1 - Article

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 2

VL - 107

SP - 375

EP - 381

ER -

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