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Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping

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Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping. / Allan, James; Fraser, Ross M.; Owen-Hughes, Tom; Keszenman-Pereyra, David.

In: Journal of Molecular Biology, Vol. 417, No. 3, 30.03.2012, p. 152-164.

Research output: Contribution to journalArticle

Harvard

Allan, J, Fraser, RM, Owen-Hughes, T & Keszenman-Pereyra, D 2012, 'Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping' Journal of Molecular Biology, vol 417, no. 3, pp. 152-164.

APA

Allan, J., Fraser, R. M., Owen-Hughes, T., & Keszenman-Pereyra, D. (2012). Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping. Journal of Molecular Biology, 417(3), 152-164doi: 10.1016/j.jmb.2012.01.043

Vancouver

Allan J, Fraser RM, Owen-Hughes T, Keszenman-Pereyra D. Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping. Journal of Molecular Biology. 2012 Mar 30;417(3):152-164.

Author

Allan, James; Fraser, Ross M.; Owen-Hughes, Tom; Keszenman-Pereyra, David / Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping.

In: Journal of Molecular Biology, Vol. 417, No. 3, 30.03.2012, p. 152-164.

Research output: Contribution to journalArticle

Bibtex - Download

@article{1b675c0f52454a64a4d5e97ae88dfaa0,
title = "Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping",
author = "James Allan and Fraser, {Ross M.} and Tom Owen-Hughes and David Keszenman-Pereyra",
year = "2012",
volume = "417",
number = "3",
pages = "152--164",
journal = "Journal of Molecular Biology",
issn = "0022-2836",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping

A1 - Allan,James

A1 - Fraser,Ross M.

A1 - Owen-Hughes,Tom

A1 - Keszenman-Pereyra,David

AU - Allan,James

AU - Fraser,Ross M.

AU - Owen-Hughes,Tom

AU - Keszenman-Pereyra,David

PY - 2012/3/30

Y1 - 2012/3/30

N2 - <p>We have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with either chicken or frog histones, were separately digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase (CAD). Both enzymes preferentially cleave internucleosomal (linker) DNA, although they do so by markedly different mechanisms. MNase has hitherto been very widely used to map nucleosomes, although concerns have been raised over its potential to introduce bias. Having identified the locations and quantified the strength of both the chicken or frog histone octamer binding sites on each DNA, the results obtained with the two enzymes were compared using a variety of criteria. Both enzymes displayed sequence specificity in their preferred cleavage sites, although the nature of this selectivity was distinct for the two enzymes. In addition, nucleosomes produced by CAD nuclease are 8-10 bp longer than those produced with MNase, with the CAD cleavage sites tending to be 4-5 bp further out from the nucleosomal dyad than the corresponding MNase cleavage sites. Despite these notable differences in cleavage behaviour, the two nucleases identified essentially equivalent patterns of nucleosome positioning sites on each of the DNAs tested, an observation that was independent of the histone type. These results indicate that biases in nucleosome positioning data collected using MNase are, under our conditions, not significant. (C) 2012 Elsevier Ltd. All rights reserved.</p>

AB - <p>We have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with either chicken or frog histones, were separately digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase (CAD). Both enzymes preferentially cleave internucleosomal (linker) DNA, although they do so by markedly different mechanisms. MNase has hitherto been very widely used to map nucleosomes, although concerns have been raised over its potential to introduce bias. Having identified the locations and quantified the strength of both the chicken or frog histone octamer binding sites on each DNA, the results obtained with the two enzymes were compared using a variety of criteria. Both enzymes displayed sequence specificity in their preferred cleavage sites, although the nature of this selectivity was distinct for the two enzymes. In addition, nucleosomes produced by CAD nuclease are 8-10 bp longer than those produced with MNase, with the CAD cleavage sites tending to be 4-5 bp further out from the nucleosomal dyad than the corresponding MNase cleavage sites. Despite these notable differences in cleavage behaviour, the two nucleases identified essentially equivalent patterns of nucleosome positioning sites on each of the DNAs tested, an observation that was independent of the histone type. These results indicate that biases in nucleosome positioning data collected using MNase are, under our conditions, not significant. (C) 2012 Elsevier Ltd. All rights reserved.</p>

UR - http://ukpmc.ac.uk/articles/PMC3314939

U2 - 10.1016/j.jmb.2012.01.043

DO - 10.1016/j.jmb.2012.01.043

M1 - Article

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

VL - 417

SP - 152

EP - 164

ER -

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