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Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples

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Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples. / Canetta, Elisabetta; Mazilu, Michael; De Luca, Anna Chiara; Carruthers, Antonia E.; Dholakia, Kishan; Neilson, Sam; Sargeant, Harry; Briscoe, Tina; Herrington, C. Simon; Riches, Andrew C.

In: Journal of Biomedical Optics, Vol. 16, No. 3, 037002, 03.2011, p. -.

Research output: Contribution to journalArticle

Harvard

Canetta, E, Mazilu, M, De Luca, AC, Carruthers, AE, Dholakia, K, Neilson, S, Sargeant, H, Briscoe, T, Herrington, CS & Riches, AC 2011, 'Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples' Journal of Biomedical Optics, vol 16, no. 3, 037002, pp. -., 10.1117/1.3556722

APA

Canetta, E., Mazilu, M., De Luca, A. C., Carruthers, A. E., Dholakia, K., Neilson, S., ... Riches, A. C. (2011). Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples. Journal of Biomedical Optics, 16(3), -. [037002]. 10.1117/1.3556722

Vancouver

Canetta E, Mazilu M, De Luca AC, Carruthers AE, Dholakia K, Neilson S et al. Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples. Journal of Biomedical Optics. 2011 Mar;16(3):-. 037002. Available from: 10.1117/1.3556722

Author

Canetta, Elisabetta; Mazilu, Michael; De Luca, Anna Chiara; Carruthers, Antonia E.; Dholakia, Kishan; Neilson, Sam; Sargeant, Harry; Briscoe, Tina; Herrington, C. Simon; Riches, Andrew C. / Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples.

In: Journal of Biomedical Optics, Vol. 16, No. 3, 037002, 03.2011, p. -.

Research output: Contribution to journalArticle

Bibtex - Download

@article{059c844e7f06498bbb42f87b8d9be8dd,
title = "Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples",
keywords = "Raman spectroscopy, modulation spectroscopy, bladder cancer, FLUORESCENCE SUPPRESSION, CANCER, REJECTION, ALGORITHM, NEOPLASIA",
author = "Elisabetta Canetta and Michael Mazilu and {De Luca}, {Anna Chiara} and Carruthers, {Antonia E.} and Kishan Dholakia and Sam Neilson and Harry Sargeant and Tina Briscoe and Herrington, {C. Simon} and Riches, {Andrew C.}",
year = "2011",
doi = "10.1117/1.3556722",
volume = "16",
number = "3",
pages = "--",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples

A1 - Canetta,Elisabetta

A1 - Mazilu,Michael

A1 - De Luca,Anna Chiara

A1 - Carruthers,Antonia E.

A1 - Dholakia,Kishan

A1 - Neilson,Sam

A1 - Sargeant,Harry

A1 - Briscoe,Tina

A1 - Herrington,C. Simon

A1 - Riches,Andrew C.

AU - Canetta,Elisabetta

AU - Mazilu,Michael

AU - De Luca,Anna Chiara

AU - Carruthers,Antonia E.

AU - Dholakia,Kishan

AU - Neilson,Sam

AU - Sargeant,Harry

AU - Briscoe,Tina

AU - Herrington,C. Simon

AU - Riches,Andrew C.

PY - 2011/3

Y1 - 2011/3

N2 - <p>Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556722]</p>

AB - <p>Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556722]</p>

KW - Raman spectroscopy

KW - modulation spectroscopy

KW - bladder cancer

KW - FLUORESCENCE SUPPRESSION

KW - CANCER

KW - REJECTION

KW - ALGORITHM

KW - NEOPLASIA

U2 - 10.1117/1.3556722

DO - 10.1117/1.3556722

M1 - Article

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 3

VL - 16

SP - -

ER -

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