Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples. / Canetta, Elisabetta; Mazilu, Michael; De Luca, Anna Chiara; Carruthers, Antonia E.; Dholakia, Kishan; Neilson, Sam; Sargeant, Harry; Briscoe, Tina; Herrington, C. Simon; Riches, Andrew C.
In: Journal of Biomedical Optics, Vol. 16, No. 3, 03.2011, p. -, 037002.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples
A1 - Canetta,Elisabetta
A1 - Mazilu,Michael
A1 - De Luca,Anna Chiara
A1 - Carruthers,Antonia E.
A1 - Dholakia,Kishan
A1 - Neilson,Sam
A1 - Sargeant,Harry
A1 - Briscoe,Tina
A1 - Herrington,C. Simon
A1 - Riches,Andrew C.
AU - Canetta,Elisabetta
AU - Mazilu,Michael
AU - De Luca,Anna Chiara
AU - Carruthers,Antonia E.
AU - Dholakia,Kishan
AU - Neilson,Sam
AU - Sargeant,Harry
AU - Briscoe,Tina
AU - Herrington,C. Simon
AU - Riches,Andrew C.
PY - 2011/3
Y1 - 2011/3
N2 - <p>Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556722]</p>
AB - <p>Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556722]</p>
KW - Raman spectroscopy
KW - modulation spectroscopy
KW - bladder cancer
KW - FLUORESCENCE SUPPRESSION
KW - CANCER
KW - REJECTION
KW - ALGORITHM
KW - NEOPLASIA
U2 - 10.1117/1.3556722
DO - 10.1117/1.3556722
M1 - Article
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 3
VL - 16
SP - -
ER -