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Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes

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Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes. / Lambert, Carey; Till, Rob; Hobley, Laura; Sockett, R. E.

In: BMC Microbiology, Vol. 12, 99, 07.06.2012, p. -.

Research output: Contribution to journalArticle

Harvard

Lambert, C, Till, R, Hobley, L & Sockett, RE 2012, 'Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes' BMC Microbiology, vol 12, 99, pp. -.

APA

Lambert, C., Till, R., Hobley, L., & Sockett, R. E. (2012). Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes. BMC Microbiology, 12, -[99]doi: 10.1186/1471-2180-12-99

Vancouver

Lambert C, Till R, Hobley L, Sockett RE. Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes. BMC Microbiology. 2012 Jun 7;12:-. 99.

Author

Lambert, Carey; Till, Rob; Hobley, Laura; Sockett, R. E. / Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes.

In: BMC Microbiology, Vol. 12, 99, 07.06.2012, p. -.

Research output: Contribution to journalArticle

Bibtex - Download

@article{030a3ac7e2e048d394611f54ba38a243,
title = "Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes",
author = "Carey Lambert and Rob Till and Laura Hobley and Sockett, {R. E.}",
year = "2012",
volume = "12",
pages = "--",
journal = "BMC Microbiology",
issn = "1471-2180",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes

A1 - Lambert,Carey

A1 - Till,Rob

A1 - Hobley,Laura

A1 - Sockett,R. E.

AU - Lambert,Carey

AU - Till,Rob

AU - Hobley,Laura

AU - Sockett,R. E.

PY - 2012/6/7

Y1 - 2012/6/7

N2 - <p>Background: Bdellovibrio bacteriovorus HD100 must regulate genes in response to a variety of environmental conditions as it enters, preys upon and leaves other bacteria, or grows axenically without prey. In addition to "housekeeping" sigma factors, its genome encodes several alternate sigma factors, including 2 Group IV-RpoE-like proteins, which may be involved in the complex regulation of its predatory lifestyle.</p><p>Results: We find that one sigma factor gene, bd3314, cannot be deleted from Bdellovibrio in either predatory or prey-independent growth states, and is therefore possibly essential, likely being an alternate sigma 70. Deletion of one of two Group IV-like sigma factor genes, bd0881, affects flagellar gene regulation and results in less efficient predation, although not due to motility changes; deletion of the second, bd0743, showed that it normally represses chaperone gene expression and intriguingly we find an alternative groES gene is expressed at timepoints in the predatory cycle where intensive protein synthesis at Bdellovibrio septation, prior to prey lysis, will be occurring.</p><p>Conclusions: We have taken the first step in understanding how alternate sigma factors regulate different processes in the predatory lifecycle of Bdellovibrio and discovered that alternate chaperones regulated by one of them are expressed at different stages of the lifecycle.</p>

AB - <p>Background: Bdellovibrio bacteriovorus HD100 must regulate genes in response to a variety of environmental conditions as it enters, preys upon and leaves other bacteria, or grows axenically without prey. In addition to "housekeeping" sigma factors, its genome encodes several alternate sigma factors, including 2 Group IV-RpoE-like proteins, which may be involved in the complex regulation of its predatory lifestyle.</p><p>Results: We find that one sigma factor gene, bd3314, cannot be deleted from Bdellovibrio in either predatory or prey-independent growth states, and is therefore possibly essential, likely being an alternate sigma 70. Deletion of one of two Group IV-like sigma factor genes, bd0881, affects flagellar gene regulation and results in less efficient predation, although not due to motility changes; deletion of the second, bd0743, showed that it normally represses chaperone gene expression and intriguingly we find an alternative groES gene is expressed at timepoints in the predatory cycle where intensive protein synthesis at Bdellovibrio septation, prior to prey lysis, will be occurring.</p><p>Conclusions: We have taken the first step in understanding how alternate sigma factors regulate different processes in the predatory lifecycle of Bdellovibrio and discovered that alternate chaperones regulated by one of them are expressed at different stages of the lifecycle.</p>

U2 - 10.1186/1471-2180-12-99

DO - 10.1186/1471-2180-12-99

M1 - Article

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

VL - 12

SP - -

ER -

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