Mutations to the histone H3 alpha N region selectively alter the outcome of ATP-dependent nucleosome-remodelling reactions

TY - JOUR

T1 - Mutations to the histone H3 alpha N region selectively alter the outcome of ATP-dependent nucleosome-remodelling reactions

A1 - Somers,Joanna

A1 - Owen-Hughes,Tom

AU - Somers,Joanna

AU - Owen-Hughes,Tom

PY - 2009/5

Y1 - 2009/5

N2 - <p>Mutational analysis of the histone H3 N-terminal region has shown it to play an important role both in chromatin function in vivo and nucleosome dynamics in vitro. Here we use a library of mutations in the H3 N-terminal region to investigate the contribution of this region to the action of the ATP-dependent remodelling enzymes Chd1, RSC and SWI/SNF. All of the enzymes were affected differently by the mutations with Chd1 being affected the least and RSC being most sensitive. In addition to affecting the rate of remodelling by RSC, some mutations prevented RSC from moving nucleosomes to locations in which DNA was unravelled. These observations illustrate that the mechanisms by which different ATP-dependent remodelling enzymes act are sensitive to different features of nucleosome structure. They also show how alterations to histones can affect the products generated as a result of ATP-dependent remodelling reactions.</p>

AB - <p>Mutational analysis of the histone H3 N-terminal region has shown it to play an important role both in chromatin function in vivo and nucleosome dynamics in vitro. Here we use a library of mutations in the H3 N-terminal region to investigate the contribution of this region to the action of the ATP-dependent remodelling enzymes Chd1, RSC and SWI/SNF. All of the enzymes were affected differently by the mutations with Chd1 being affected the least and RSC being most sensitive. In addition to affecting the rate of remodelling by RSC, some mutations prevented RSC from moving nucleosomes to locations in which DNA was unravelled. These observations illustrate that the mechanisms by which different ATP-dependent remodelling enzymes act are sensitive to different features of nucleosome structure. They also show how alterations to histones can affect the products generated as a result of ATP-dependent remodelling reactions.</p>

KW - BASE-PAIR RESOLUTION

KW - DNA TRANSLOCATION

KW - SNF2 FAMILY

KW - CHROMATIN

KW - STABILITY

KW - SWI/SNF

KW - RSC

KW - SUBFAMILIES

KW - SEQUENCES

KW - PROTEINS

UR - http://ukpmc.ac.uk/articles/PMC2677872

U2 - 10.1093/nar/gkp114

DO - 10.1093/nar/gkp114

M1 - Article

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 8

VL - 37

SP - 2504

EP - 2513

ER -