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N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels

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N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels. / Perez-Mendoza, Daniel; Coulthurst, Sarah J.; Sanjuan, Juan; Salmond, George P. C.

In: Microbiology-SGM, Vol. 157, No. 12, 12.2011, p. 3340-3348.

Research output: Contribution to journalArticle

Harvard

Perez-Mendoza, D, Coulthurst, SJ, Sanjuan, J & Salmond, GPC 2011, 'N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels' Microbiology-SGM, vol 157, no. 12, pp. 3340-3348.

APA

Perez-Mendoza, D., Coulthurst, S. J., Sanjuan, J., & Salmond, G. P. C. (2011). N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels. Microbiology-SGM, 157(12), 3340-3348doi: 10.1099/mic.0.050450-0

Vancouver

Perez-Mendoza D, Coulthurst SJ, Sanjuan J, Salmond GPC. N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels. Microbiology-SGM. 2011 Dec;157(12):3340-3348.

Author

Perez-Mendoza, Daniel; Coulthurst, Sarah J.; Sanjuan, Juan; Salmond, George P. C. / N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels.

In: Microbiology-SGM, Vol. 157, No. 12, 12.2011, p. 3340-3348.

Research output: Contribution to journalArticle

Bibtex - Download

@article{aa2ead0ef3fd452e8f76c5c00737cb3e,
title = "N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels",
author = "Daniel Perez-Mendoza and Coulthurst, {Sarah J.} and Juan Sanjuan and Salmond, {George P. C.}",
year = "2011",
volume = "157",
number = "12",
pages = "3340--3348",
journal = "Microbiology-SGM",
issn = "1350-0872",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels

A1 - Perez-Mendoza,Daniel

A1 - Coulthurst,Sarah J.

A1 - Sanjuan,Juan

A1 - Salmond,George P. C.

AU - Perez-Mendoza,Daniel

AU - Coulthurst,Sarah J.

AU - Sanjuan,Juan

AU - Salmond,George P. C.

PY - 2011/12

Y1 - 2011/12

N2 - <p>The phytopathogenic bacterium Pectobacterium atrosepticum (Pba) strain SCRI1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. Here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-GMP, through overexpression of a constitutively active diguanylate cyclase (PleD*) from Caulobacter crescentus. Randomly obtained Pba transposon mutants defective in the pga operon, involved in synthesis and translocation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), were all impaired in this biofilm formation. The presence of the PGA-degrading enzyme dispersin B in the growth media prevented biofilm formation by Pba overexpressing PleD*, further supporting the importance of PGA for biofilm formation by Pba. Importantly, a pga mutant exhibited a reduction in root binding to the host plant under conditions of high intracellular c-di-GMP levels. A modest but consistent increase in pga transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in Pba.</p>

AB - <p>The phytopathogenic bacterium Pectobacterium atrosepticum (Pba) strain SCRI1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. Here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-GMP, through overexpression of a constitutively active diguanylate cyclase (PleD*) from Caulobacter crescentus. Randomly obtained Pba transposon mutants defective in the pga operon, involved in synthesis and translocation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), were all impaired in this biofilm formation. The presence of the PGA-degrading enzyme dispersin B in the growth media prevented biofilm formation by Pba overexpressing PleD*, further supporting the importance of PGA for biofilm formation by Pba. Importantly, a pga mutant exhibited a reduction in root binding to the host plant under conditions of high intracellular c-di-GMP levels. A modest but consistent increase in pga transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in Pba.</p>

KW - CAROTOVORA SUBSP-ATROSEPTICA

KW - YERSINIA-PESTIS BIOFILM

KW - CYCLIC DIGUANYLIC ACID

KW - ESCHERICHIA-COLI

KW - ERWINIA-CAROTOVORA

KW - PSEUDOMONAS-AERUGINOSA

KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

KW - CELLULOSE SYNTHESIS

KW - EXOPOLYSACCHARIDE PRODUCTION

KW - RHIZOBIUM-LEGUMINOSARUM

U2 - 10.1099/mic.0.050450-0

DO - 10.1099/mic.0.050450-0

M1 - Article

JO - Microbiology-SGM

JF - Microbiology-SGM

SN - 1350-0872

IS - 12

VL - 157

SP - 3340

EP - 3348

ER -

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