N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels. / Perez-Mendoza, Daniel; Coulthurst, Sarah J.; Sanjuan, Juan; Salmond, George P. C.
In: Microbiology, Vol. 157, No. 12, 12.2011, p. 3340-3348.Research output: Contribution to journal › Article
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TY - JOUR
T1 - N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels
AU - Perez-Mendoza,Daniel
AU - Coulthurst,Sarah J.
AU - Sanjuan,Juan
AU - Salmond,George P. C.
PY - 2011/12
Y1 - 2011/12
N2 - The phytopathogenic bacterium Pectobacterium atrosepticum (Pba) strain SCRI1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. Here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-GMP, through overexpression of a constitutively active diguanylate cyclase (PleD*) from Caulobacter crescentus. Randomly obtained Pba transposon mutants defective in the pga operon, involved in synthesis and translocation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), were all impaired in this biofilm formation. The presence of the PGA-degrading enzyme dispersin B in the growth media prevented biofilm formation by Pba overexpressing PleD*, further supporting the importance of PGA for biofilm formation by Pba. Importantly, a pga mutant exhibited a reduction in root binding to the host plant under conditions of high intracellular c-di-GMP levels. A modest but consistent increase in pga transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in Pba.
AB - The phytopathogenic bacterium Pectobacterium atrosepticum (Pba) strain SCRI1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. Here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-GMP, through overexpression of a constitutively active diguanylate cyclase (PleD*) from Caulobacter crescentus. Randomly obtained Pba transposon mutants defective in the pga operon, involved in synthesis and translocation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), were all impaired in this biofilm formation. The presence of the PGA-degrading enzyme dispersin B in the growth media prevented biofilm formation by Pba overexpressing PleD*, further supporting the importance of PGA for biofilm formation by Pba. Importantly, a pga mutant exhibited a reduction in root binding to the host plant under conditions of high intracellular c-di-GMP levels. A modest but consistent increase in pga transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in Pba.
KW - CAROTOVORA SUBSP-ATROSEPTICA
KW - YERSINIA-PESTIS BIOFILM
KW - CYCLIC DIGUANYLIC ACID
KW - ESCHERICHIA-COLI
KW - ERWINIA-CAROTOVORA
KW - PSEUDOMONAS-AERUGINOSA
KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
KW - CELLULOSE SYNTHESIS
KW - EXOPOLYSACCHARIDE PRODUCTION
KW - RHIZOBIUM-LEGUMINOSARUM
U2 - 10.1099/mic.0.050450-0
DO - 10.1099/mic.0.050450-0
M3 - Article
VL - 157
SP - 3340
EP - 3348
JO - Microbiology
T2 - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 12
ER -