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Novel bacteriophages containing a genome of another bacteriophage within their genomes

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Novel bacteriophages containing a genome of another bacteriophage within their genomes. / Swanson, Maud M.; Reavy, Brian; Makarova, Kira S.; Cock, Peter J.; Hopkins, David W.; Torrance, Lesley; Koonin, Eugene V.; Taliansky, Michael.

In: PLoS ONE, Vol. 7, No. 7, e40683, 17.07.2012, p. -.

Research output: Contribution to journalArticle

Harvard

Swanson, MM, Reavy, B, Makarova, KS, Cock, PJ, Hopkins, DW, Torrance, L, Koonin, EV & Taliansky, M 2012, 'Novel bacteriophages containing a genome of another bacteriophage within their genomes' PLoS ONE, vol 7, no. 7, e40683, pp. -.

APA

Swanson, M. M., Reavy, B., Makarova, K. S., Cock, P. J., Hopkins, D. W., Torrance, L., Koonin, E. V., & Taliansky, M. (2012). Novel bacteriophages containing a genome of another bacteriophage within their genomes. PLoS ONE, 7(7), -[e40683]doi: 10.1371/journal.pone.0040683

Vancouver

Swanson MM, Reavy B, Makarova KS, Cock PJ, Hopkins DW, Torrance L et al. Novel bacteriophages containing a genome of another bacteriophage within their genomes. PLoS ONE. 2012 Jul 17;7(7):-. e40683.

Author

Swanson, Maud M.; Reavy, Brian; Makarova, Kira S.; Cock, Peter J.; Hopkins, David W.; Torrance, Lesley; Koonin, Eugene V.; Taliansky, Michael / Novel bacteriophages containing a genome of another bacteriophage within their genomes.

In: PLoS ONE, Vol. 7, No. 7, e40683, 17.07.2012, p. -.

Research output: Contribution to journalArticle

Bibtex - Download

@article{8d946157500140569f819bc9c8dee9a2,
title = "Novel bacteriophages containing a genome of another bacteriophage within their genomes",
author = "Swanson, {Maud M.} and Brian Reavy and Makarova, {Kira S.} and Cock, {Peter J.} and Hopkins, {David W.} and Lesley Torrance and Koonin, {Eugene V.} and Michael Taliansky",
year = "2012",
volume = "7",
number = "7",
pages = "--",
journal = "PLoS ONE",
issn = "1932-6203",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Novel bacteriophages containing a genome of another bacteriophage within their genomes

A1 - Swanson,Maud M.

A1 - Reavy,Brian

A1 - Makarova,Kira S.

A1 - Cock,Peter J.

A1 - Hopkins,David W.

A1 - Torrance,Lesley

A1 - Koonin,Eugene V.

A1 - Taliansky,Michael

AU - Swanson,Maud M.

AU - Reavy,Brian

AU - Makarova,Kira S.

AU - Cock,Peter J.

AU - Hopkins,David W.

AU - Torrance,Lesley

AU - Koonin,Eugene V.

AU - Taliansky,Michael

PY - 2012/7/17

Y1 - 2012/7/17

N2 - <p>A novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 39 protruding cohesive ends. The SpaA1 genome encompasses 63 predicted protein-coding genes which cluster within three regions of the genome, each of apparently different origin, in a mosaic pattern. In two of these regions, the gene sets resemble those in prophages of Bacillus thuringiensis kurstaki str. T03a001 (genes involved in DNA replication/transcription, cell entry and exit) and B. cereus AH676 (additional regulatory and recombination genes), respectively. The third region represents an almost complete genome (except for the short terminal segments) of a distinct bacteriophage, MZTP02. Nearly the same gene module was identified in prophages of B. thuringiensis serovar monterrey BGSC 4AJ1 and B. cereus Rock4-2. These findings suggest that MZTP02 can be shuttled between genomes of other bacteriophages and prophages, leading to the formation of chimeric genomes. The presence of a complete phage genome in the genome of other phages apparently has not been described previously and might represent a 'fast track' route of virus evolution and horizontal gene transfer. Another phage (BceA1) nearly identical in sequence to SpaA1, and also including the almost complete MZTP02 genome within its own genome, was isolated from a bacterium of the B. cereus/B. thuringiensis group. Remarkably, both SpaA1 and BceA1 phages can infect B. cereus and B. thuringiensis, but only one of them, SpaA1, can infect S. pasteuri. This finding is best compatible with a scenario in which MZTP02 was originally contained in BceA1 infecting Bacillus spp, the common hosts for these two phages, followed by emergence of SpaA1 infecting S. pasteuri.</p>

AB - <p>A novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 39 protruding cohesive ends. The SpaA1 genome encompasses 63 predicted protein-coding genes which cluster within three regions of the genome, each of apparently different origin, in a mosaic pattern. In two of these regions, the gene sets resemble those in prophages of Bacillus thuringiensis kurstaki str. T03a001 (genes involved in DNA replication/transcription, cell entry and exit) and B. cereus AH676 (additional regulatory and recombination genes), respectively. The third region represents an almost complete genome (except for the short terminal segments) of a distinct bacteriophage, MZTP02. Nearly the same gene module was identified in prophages of B. thuringiensis serovar monterrey BGSC 4AJ1 and B. cereus Rock4-2. These findings suggest that MZTP02 can be shuttled between genomes of other bacteriophages and prophages, leading to the formation of chimeric genomes. The presence of a complete phage genome in the genome of other phages apparently has not been described previously and might represent a 'fast track' route of virus evolution and horizontal gene transfer. Another phage (BceA1) nearly identical in sequence to SpaA1, and also including the almost complete MZTP02 genome within its own genome, was isolated from a bacterium of the B. cereus/B. thuringiensis group. Remarkably, both SpaA1 and BceA1 phages can infect B. cereus and B. thuringiensis, but only one of them, SpaA1, can infect S. pasteuri. This finding is best compatible with a scenario in which MZTP02 was originally contained in BceA1 infecting Bacillus spp, the common hosts for these two phages, followed by emergence of SpaA1 infecting S. pasteuri.</p>

U2 - 10.1371/journal.pone.0040683

DO - 10.1371/journal.pone.0040683

M1 - Article

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 7

VL - 7

SP - -

ER -

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