Optical separation of cells on potential energy landscapes : Enhancement with dielectric tagging. / Dholakia, Kishan; Lee, Woei Ming; Paterson, Lynn; MacDonald, Michael P.; McDonald, Richard; Andreev, Igor; Mthunzi, Patience; Brown, C. Tom A.; Marchington, Robert F.; Riches, Andrew C.
In: IEEE Journal of Selected Topics in Quantum Electronics, Vol. 13, No. 6, 2007, p. 1646-1654.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Optical separation of cells on potential energy landscapes
T2 - Enhancement with dielectric tagging
A1 - Dholakia,Kishan
A1 - Lee,Woei Ming
A1 - Paterson,Lynn
A1 - MacDonald,Michael P.
A1 - McDonald,Richard
A1 - Andreev,Igor
A1 - Mthunzi,Patience
A1 - Brown,C. Tom A.
A1 - Marchington,Robert F.
A1 - Riches,Andrew C.
AU - Dholakia,Kishan
AU - Lee,Woei Ming
AU - Paterson,Lynn
AU - MacDonald,Michael P.
AU - McDonald,Richard
AU - Andreev,Igor
AU - Mthunzi,Patience
AU - Brown,C. Tom A.
AU - Marchington,Robert F.
AU - Riches,Andrew C.
PY - 2007
Y1 - 2007
N2 - <p>We review the emergent techniques of microfluidic sorting of colloidal and cellular samples using optical forces. We distinguish between what we term as passive and active forms of particle sorting where we can sort either with the use of a fluorescent marker (active) or based on physical attributes alone (passive). We then examine cell sorting with optical potential landscapes such as a Bessel light beam and a multibeam interference pattern. For both forms of optical potential energy landscape, we further present the possibility of enhancing the optical sorting process by tagging dielectric microspheres onto the cells. The results suggest that the methodology of tagging can enhance the sorting of cells as they subsequently respond more strongly to an applied optical field or potential energy landscape. This technique presents a simple method to enhance the sorting process.</p>
AB - <p>We review the emergent techniques of microfluidic sorting of colloidal and cellular samples using optical forces. We distinguish between what we term as passive and active forms of particle sorting where we can sort either with the use of a fluorescent marker (active) or based on physical attributes alone (passive). We then examine cell sorting with optical potential landscapes such as a Bessel light beam and a multibeam interference pattern. For both forms of optical potential energy landscape, we further present the possibility of enhancing the optical sorting process by tagging dielectric microspheres onto the cells. The results suggest that the methodology of tagging can enhance the sorting of cells as they subsequently respond more strongly to an applied optical field or potential energy landscape. This technique presents a simple method to enhance the sorting process.</p>
KW - cell tagging
KW - flow cytometry
KW - optical sorting
KW - optical trapping
KW - optical tweezers
KW - MAMMALIAN-CELLS
KW - MANIPULATION
KW - PARTICLES
KW - LIGHT
KW - CHROMATOGRAPHY
KW - TWEEZERS
KW - MICROMANIPULATION
KW - MICROPARTICLES
KW - EXCITATION
KW - DELIVERY
U2 - 10.1109/JSTQE.2007.911314
DO - 10.1109/JSTQE.2007.911314
M1 - Article
JO - IEEE Journal of Selected Topics in Quantum Electronics
JF - IEEE Journal of Selected Topics in Quantum Electronics
SN - 1077-260X
IS - 6
VL - 13
SP - 1646
EP - 1654
ER -