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Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

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Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. / El-Ghobashy, Alaa A.; Shaaban, Abeer M.; Herod, Jonathan; Innes, Jenny; Prime, Wendy; Herrington, C. Simon.

In: Gynecologic Oncology, Vol. 92, No. 2, 2004, p. 628-634.

Research output: Contribution to journalArticle

Harvard

El-Ghobashy, AA, Shaaban, AM, Herod, J, Innes, J, Prime, W & Herrington, CS 2004, 'Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix' Gynecologic Oncology, vol 92, no. 2, pp. 628-634. DOI: 10.1016/j.ygyno.2003.11.010

APA

El-Ghobashy, A. A., Shaaban, A. M., Herod, J., Innes, J., Prime, W., & Herrington, C. S. (2004). Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. Gynecologic Oncology, 92(2), 628-634. DOI: 10.1016/j.ygyno.2003.11.010

Vancouver

El-Ghobashy AA, Shaaban AM, Herod J, Innes J, Prime W, Herrington CS. Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. Gynecologic Oncology. 2004;92(2):628-634. Available from, DOI: 10.1016/j.ygyno.2003.11.010

Author

El-Ghobashy, Alaa A.; Shaaban, Abeer M.; Herod, Jonathan; Innes, Jenny; Prime, Wendy; Herrington, C. Simon / Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix.

In: Gynecologic Oncology, Vol. 92, No. 2, 2004, p. 628-634.

Research output: Contribution to journalArticle

Bibtex - Download

@article{71f3f6d0802e4e7d8e74b6d38d2d5698,
title = "Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix",
author = "El-Ghobashy, {Alaa A.} and Shaaban, {Abeer M.} and Jonathan Herod and Jenny Innes and Wendy Prime and Herrington, {C. Simon}",
year = "2004",
doi = "10.1016/j.ygyno.2003.11.010",
volume = "92",
pages = "628--634",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Elsevier",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

AU - El-Ghobashy,Alaa A.

AU - Shaaban,Abeer M.

AU - Herod,Jonathan

AU - Innes,Jenny

AU - Prime,Wendy

AU - Herrington,C. Simon

PY - 2004

Y1 - 2004

N2 - Introduction. Cyclins are a family of regulatory proteins that play a pivotal role in controlling the cell cycle. While there is evidence of their altered expression in cervical squamous lesions, their precise role in glandular neoplasia is yet to be elucidated. Objectives. To investigate the role of cyclins as markers of early cervical glandular neoplasia by comparing their expression in lesions of different histological type. Methods. Through a cross-sectional analytical study, paraffin wax sections of normal cervix (n = 11), endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive adenocarcinoma (n = 28) were studied using monoclonal antibodies for cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A quantitative assessment was employed for the analysis of percentage expression of each marker. Statistical analysis of data was performed using SPSS. Results. A progressive significant increase in cyclin A expression occurred from normal cervix (median: 0, IQ: 0-0), through endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). Statistically higher expression of cyclin B was found in CGIN than in TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed higher levels of cyclins A and B than CGIN (P < 0.001). There was significantly greater cyclin E expression in TEM/endometriosis than in normal cervix (P = 0.03) with a nonsignificant further increase in CGIN and invasive adenocarcinoma. The expression of cyclin D was not significantly different among all groups. Conclusions. Our data indicate that up-regulation of cyclin A and B expression occurs in neoplastic lesions of the cervix. Cyclin B expression was significantly more widespread in CGIN lesions than in TEM/endometriosis indicating that further assessment of the value of this marker in the diagnosis of cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All rights reserved.

AB - Introduction. Cyclins are a family of regulatory proteins that play a pivotal role in controlling the cell cycle. While there is evidence of their altered expression in cervical squamous lesions, their precise role in glandular neoplasia is yet to be elucidated. Objectives. To investigate the role of cyclins as markers of early cervical glandular neoplasia by comparing their expression in lesions of different histological type. Methods. Through a cross-sectional analytical study, paraffin wax sections of normal cervix (n = 11), endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive adenocarcinoma (n = 28) were studied using monoclonal antibodies for cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A quantitative assessment was employed for the analysis of percentage expression of each marker. Statistical analysis of data was performed using SPSS. Results. A progressive significant increase in cyclin A expression occurred from normal cervix (median: 0, IQ: 0-0), through endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). Statistically higher expression of cyclin B was found in CGIN than in TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed higher levels of cyclins A and B than CGIN (P < 0.001). There was significantly greater cyclin E expression in TEM/endometriosis than in normal cervix (P = 0.03) with a nonsignificant further increase in CGIN and invasive adenocarcinoma. The expression of cyclin D was not significantly different among all groups. Conclusions. Our data indicate that up-regulation of cyclin A and B expression occurs in neoplastic lesions of the cervix. Cyclin B expression was significantly more widespread in CGIN lesions than in TEM/endometriosis indicating that further assessment of the value of this marker in the diagnosis of cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.ygyno.2003.11.010

DO - 10.1016/j.ygyno.2003.11.010

M3 - Article

VL - 92

SP - 628

EP - 634

JO - Gynecologic Oncology

T2 - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

ER -

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