**Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix.** / El-Ghobashy, Alaa A.; Shaaban, Abeer M.; Herod, Jonathan; Innes, Jenny; Prime, Wendy; Herrington, C. Simon.

Research output: Contribution to journal › Article

El-Ghobashy, AA, Shaaban, AM, Herod, J, Innes, J, Prime, W & Herrington, CS 2004, 'Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix' *Gynecologic Oncology*, vol 92, no. 2, pp. 628-634. DOI: 10.1016/j.ygyno.2003.11.010

El-Ghobashy, A. A., Shaaban, A. M., Herod, J., Innes, J., Prime, W., & Herrington, C. S. (2004). *Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix*. *Gynecologic Oncology*, *92*(2), 628-634. DOI: 10.1016/j.ygyno.2003.11.010

El-Ghobashy AA, Shaaban AM, Herod J, Innes J, Prime W, Herrington CS. Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. Gynecologic Oncology. 2004;92(2):628-634. Available from, DOI: 10.1016/j.ygyno.2003.11.010

@article{71f3f6d0802e4e7d8e74b6d38d2d5698,

title = "Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix",

author = "El-Ghobashy, {Alaa A.} and Shaaban, {Abeer M.} and Jonathan Herod and Jenny Innes and Wendy Prime and Herrington, {C. Simon}",

year = "2004",

doi = "10.1016/j.ygyno.2003.11.010",

volume = "92",

pages = "628--634",

journal = "Gynecologic Oncology",

issn = "0090-8258",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

AU - El-Ghobashy,Alaa A.

AU - Shaaban,Abeer M.

AU - Herod,Jonathan

AU - Innes,Jenny

AU - Prime,Wendy

AU - Herrington,C. Simon

PY - 2004

Y1 - 2004

N2 - <p>Introduction. Cyclins are a family of regulatory proteins that play a <br/> pivotal role in controlling the cell cycle. While there is evidence of <br/> their altered expression in cervical squamous lesions, their precise <br/> role in glandular neoplasia is yet to be elucidated. Objectives. To <br/> investigate the role of cyclins as markers of early cervical glandular <br/> neoplasia by comparing their expression in lesions of different <br/> histological type. Methods. Through a cross-sectional analytical study, <br/> paraffin wax sections of normal cervix (n = 11), <br/> endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical <br/> glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive <br/> adenocarcinoma (n = 28) were studied using monoclonal antibodies for <br/> cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A <br/> quantitative assessment was employed for the analysis of percentage <br/> expression of each marker. Statistical analysis of data was performed <br/> using SPSS. Results. A progressive significant increase in cyclin A <br/> expression occurred from normal cervix (median: 0, IQ: 0-0), through <br/> endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) <br/> to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B <br/> exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, <br/> median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). <br/> Statistically higher expression of cyclin B was found in CGIN than in <br/> TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed <br/> higher levels of cyclins A and B than CGIN (P < 0.001). There was <br/> significantly greater cyclin E expression in TEM/endometriosis than in <br/> normal cervix (P = 0.03) with a nonsignificant further increase in CGIN <br/> and invasive adenocarcinoma. The expression of cyclin D was not <br/> significantly different among all groups. Conclusions. Our data indicate<br/> that up-regulation of cyclin A and B expression occurs in neoplastic <br/> lesions of the cervix. Cyclin B expression was significantly more <br/> widespread in CGIN lesions than in TEM/endometriosis indicating that <br/> further assessment of the value of this marker in the diagnosis of <br/> cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All <br/> rights reserved.</p>

AB - <p>Introduction. Cyclins are a family of regulatory proteins that play a <br/> pivotal role in controlling the cell cycle. While there is evidence of <br/> their altered expression in cervical squamous lesions, their precise <br/> role in glandular neoplasia is yet to be elucidated. Objectives. To <br/> investigate the role of cyclins as markers of early cervical glandular <br/> neoplasia by comparing their expression in lesions of different <br/> histological type. Methods. Through a cross-sectional analytical study, <br/> paraffin wax sections of normal cervix (n = 11), <br/> endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical <br/> glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive <br/> adenocarcinoma (n = 28) were studied using monoclonal antibodies for <br/> cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A <br/> quantitative assessment was employed for the analysis of percentage <br/> expression of each marker. Statistical analysis of data was performed <br/> using SPSS. Results. A progressive significant increase in cyclin A <br/> expression occurred from normal cervix (median: 0, IQ: 0-0), through <br/> endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) <br/> to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B <br/> exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, <br/> median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). <br/> Statistically higher expression of cyclin B was found in CGIN than in <br/> TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed <br/> higher levels of cyclins A and B than CGIN (P < 0.001). There was <br/> significantly greater cyclin E expression in TEM/endometriosis than in <br/> normal cervix (P = 0.03) with a nonsignificant further increase in CGIN <br/> and invasive adenocarcinoma. The expression of cyclin D was not <br/> significantly different among all groups. Conclusions. Our data indicate<br/> that up-regulation of cyclin A and B expression occurs in neoplastic <br/> lesions of the cervix. Cyclin B expression was significantly more <br/> widespread in CGIN lesions than in TEM/endometriosis indicating that <br/> further assessment of the value of this marker in the diagnosis of <br/> cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All <br/> rights reserved.</p>

U2 - 10.1016/j.ygyno.2003.11.010

DO - 10.1016/j.ygyno.2003.11.010

M3 - Article

VL - 92

SP - 628

EP - 634

JO - Gynecologic Oncology

T2 - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

ER -