Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

TY - JOUR

T1 - Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

A1 - El-Ghobashy,Alaa A.

A1 - Shaaban,Abeer M.

A1 - Herod,Jonathan

A1 - Innes,Jenny

A1 - Prime,Wendy

A1 - Herrington,C. Simon

AU - El-Ghobashy,Alaa A.

AU - Shaaban,Abeer M.

AU - Herod,Jonathan

AU - Innes,Jenny

AU - Prime,Wendy

AU - Herrington,C. Simon

PY - 2004

Y1 - 2004

N2 - <p>Introduction. Cyclins are a family of regulatory proteins that play a <br/> pivotal role in controlling the cell cycle. While there is evidence of <br/> their altered expression in cervical squamous lesions, their precise <br/> role in glandular neoplasia is yet to be elucidated. Objectives. To <br/> investigate the role of cyclins as markers of early cervical glandular <br/> neoplasia by comparing their expression in lesions of different <br/> histological type. Methods. Through a cross-sectional analytical study, <br/> paraffin wax sections of normal cervix (n = 11), <br/> endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical <br/> glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive <br/> adenocarcinoma (n = 28) were studied using monoclonal antibodies for <br/> cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A <br/> quantitative assessment was employed for the analysis of percentage <br/> expression of each marker. Statistical analysis of data was performed <br/> using SPSS. Results. A progressive significant increase in cyclin A <br/> expression occurred from normal cervix (median: 0, IQ: 0-0), through <br/> endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) <br/> to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B <br/> exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, <br/> median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). <br/> Statistically higher expression of cyclin B was found in CGIN than in <br/> TEM/endometriosis (P &lt; 0.001). Invasive adenocarcinomas expressed <br/> higher levels of cyclins A and B than CGIN (P &lt; 0.001). There was <br/> significantly greater cyclin E expression in TEM/endometriosis than in <br/> normal cervix (P = 0.03) with a nonsignificant further increase in CGIN <br/> and invasive adenocarcinoma. The expression of cyclin D was not <br/> significantly different among all groups. Conclusions. Our data indicate<br/> that up-regulation of cyclin A and B expression occurs in neoplastic <br/> lesions of the cervix. Cyclin B expression was significantly more <br/> widespread in CGIN lesions than in TEM/endometriosis indicating that <br/> further assessment of the value of this marker in the diagnosis of <br/> cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All <br/> rights reserved.</p>

AB - <p>Introduction. Cyclins are a family of regulatory proteins that play a <br/> pivotal role in controlling the cell cycle. While there is evidence of <br/> their altered expression in cervical squamous lesions, their precise <br/> role in glandular neoplasia is yet to be elucidated. Objectives. To <br/> investigate the role of cyclins as markers of early cervical glandular <br/> neoplasia by comparing their expression in lesions of different <br/> histological type. Methods. Through a cross-sectional analytical study, <br/> paraffin wax sections of normal cervix (n = 11), <br/> endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical <br/> glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive <br/> adenocarcinoma (n = 28) were studied using monoclonal antibodies for <br/> cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A <br/> quantitative assessment was employed for the analysis of percentage <br/> expression of each marker. Statistical analysis of data was performed <br/> using SPSS. Results. A progressive significant increase in cyclin A <br/> expression occurred from normal cervix (median: 0, IQ: 0-0), through <br/> endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) <br/> to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B <br/> exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, <br/> median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). <br/> Statistically higher expression of cyclin B was found in CGIN than in <br/> TEM/endometriosis (P &lt; 0.001). Invasive adenocarcinomas expressed <br/> higher levels of cyclins A and B than CGIN (P &lt; 0.001). There was <br/> significantly greater cyclin E expression in TEM/endometriosis than in <br/> normal cervix (P = 0.03) with a nonsignificant further increase in CGIN <br/> and invasive adenocarcinoma. The expression of cyclin D was not <br/> significantly different among all groups. Conclusions. Our data indicate<br/> that up-regulation of cyclin A and B expression occurs in neoplastic <br/> lesions of the cervix. Cyclin B expression was significantly more <br/> widespread in CGIN lesions than in TEM/endometriosis indicating that <br/> further assessment of the value of this marker in the diagnosis of <br/> cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All <br/> rights reserved.</p>

U2 - 10.1016/j.ygyno.2003.11.010

DO - 10.1016/j.ygyno.2003.11.010

M1 - Article

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 2

VL - 92

SP - 628

EP - 634

ER -