Overlapping functions of components of a bacterial Sec-independent protein export pathway. / Sargent, Frank; Bogsch, Erik G.; Stanley, Nicola R.; Wexler, Margaret; Robinson, Colin; Berks, Ben C.; Palmer, Tracy.
In: EMBO Journal, Vol. 17, No. 13, 1998, p. 3640-3650.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overlapping functions of components of a bacterial Sec-independent protein export pathway
A1 - Sargent,Frank
A1 - Bogsch,Erik G.
A1 - Stanley,Nicola R.
A1 - Wexler,Margaret
A1 - Robinson,Colin
A1 - Berks,Ben C.
A1 - Palmer,Tracy
AU - Sargent,Frank
AU - Bogsch,Erik G.
AU - Stanley,Nicola R.
AU - Wexler,Margaret
AU - Robinson,Colin
AU - Berks,Ben C.
AU - Palmer,Tracy
PY - 1998
Y1 - 1998
N2 - We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.
AB - We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.
U2 - 10.1093/emboj/17.13.3640
DO - 10.1093/emboj/17.13.3640
M1 - Article
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 13
VL - 17
SP - 3640
EP - 3650
ER -